Influence of short-term exposure to low levels of 17α-ethynylestradiol on expression of genes involved in immunity and on immune parameters in rainbow trout, Oncorhynchus mykiss

Massart, Sophie; Milla, Sylvain; Redivo, Baptiste; Flamion, Enora; Mandiki, S.N.M.; Falisse, Elodie; Kestemont, Patrick

doi:10.1016/j.aquatox.2014.10.003

Cited by 22 publications

(5 citation statements)

References 86 publications

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“…However, notice the absence of correlation between lysozyme mRNA abundance and lysozyme activity, which has ever been observed in fish (e.g. Massart et al, 2014) and might reflect post transcriptional and post-translational changes. The regulation of lysozyme in the liver and spleen has been reported following challenge tests with pathogens or LPS exposure in fish (Saurabh et al, 2011;Hang et al, 2013), this may support the role of tissular lysozyme in the fish immune response.…”

Section: Corticosteroids Do Not Appear As Strong Regulators Of Innate...mentioning

confidence: 83%

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Milla

Massart

Mathieu

et al. 2018

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Section: Corticosteroids Do Not Appear As Strong Regulators Of Innate...mentioning

confidence: 83%

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Milla

Massart

Mathieu

et al. 2018

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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“…It is still possible that there are sex-specific susceptibilities to combined effects of EE2 and other environmental stressors. Other types of environmental stressors such as microbes [39], temperature variations [27], or other micropollutants [40,46] could interact with the effects of EE2 and thereby amplify its toxicity [47,48]. Therefore, single-factor laboratory studies like ours are likely to underestimate the ecotoxicological relevance of EE2 in the wild.…”

Section: Discussionmentioning

confidence: 89%

Low adaptive potential for tolerance to ethynylestradiol, but also low toxicity, in a grayling population (Thymallus thymallus)

Cunha

Maitre

Wedekind

2019

Preprint

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The presence of a novel pollutant can induce rapid evolution if there is additive genetic variance for the tolerance to the stressor. Continuous selection over some generations can then reduce the toxicity of the pollutant but also deplete the additive genetic variance for the tolerance and thereby slow down adaptation. One common pollutant that has been ecologically relevant for some time is 17alpha-ethynyestradiol (EE2), a synthetic compound of oral contraceptives since their market launch in the 1960s. EE2 is typically found in higher concentrations in rivers than in lakes. Recent experimental work revealed significant genetic variance for the tolerance to EE2 in two lake-spawning salmonid species but no such variance in river-spawning brown trout. We used another river-spawning salmonid, the European grayling Thymallus thymallus , to study the toxicity of an ecologically relevant concentration of EE2. We also used a full-factorial in vitro breeding design and singly rearing of 1,555 embryos and larvae of 40 sib groups to test whether there is additive genetic variance for the tolerance to this pollutant. We found that exposure to EE2 reduced larval growth after hatching, but contrary to what has been found in the other salmonids, there were no significant effects of EE2 on embryo growth and survival. We found additive genetic variance for embryo viability, i.e. heritability for fitness. However, there was no significant additive variance for the tolerance to EE2. Our findings support the hypothesis that continuous selection has reduced the toxicity of EE2 and depleted genetic variance for tolerance to this synthetic stressor.

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“…Regarding our first main question: Our study adds the grayling to the list of salmonids whose embryos and larvae are susceptible to ecologically relevant concentrations of EE2, like whitefish [17], Atlantic salmon (Salmo salar) [37,38], rainbow trout (Oncorhynchus mykiss) [39], and brown trout [11]. With the present study, at least one species of each subfamily of the Salmonidae (Coregoninae, Salmoninae, and Thymallinae) has now been tested on the same ecologically relevant concentration of EE2, applied in a one-dose exposure of 2 pg to embryos developing in 2 mL wells [11,17], and at least two further species (the lake char Salvelinus umbla from Lake Geneva and the whitefish C.…”

Section: Discussionmentioning

confidence: 99%

Low adaptive potential for tolerance to ethynylestradiol, but also low toxicity, in a grayling population (Thymallus thymallus)

Wedekind

Cunha

Maitre

2019

Preprint

View full text Add to dashboard Cite

The presence of a novel pollutant can induce rapid evolution if there is additive genetic variance for the tolerance to the stressor. Continuous selection over some generations can then reduce the toxicity of the pollutant but also deplete the additive genetic variance for the tolerance and thereby slow down adaptation. One common pollutant that has been ecologically relevant for some time is 17alpha-ethynyestradiol (EE2), a synthetic compound of oral contraceptives since their market launch in the 1960s. EE2 is typically found in higher concentrations in rivers than in lakes. Recent experimental work revealed significant genetic variance for the tolerance to EE2 in two lake-spawning salmonid species but no such variation in river-spawning brown trout. We used another river-spawning salmonid, the European grayling Thymallus thymallus, to study the toxicity of an ecologically relevant concentration of EE2. We also used a full-factorial in vitro breeding design and singly rearing of 1,555 embryos and larvae of 40 sib groups to test whether there is additive genetic variance for the tolerance to this pollutant. We found that exposure to EE2 reduced larval growth after hatching, but contrary to what has been found in the other salmonids, there were no significant effects of EE2 on embryo growth and survival. We found additive genetic variance for embryo viability, i.e. heritability for fitness. However, there was no significant additive variance for the tolerance to EE2. Our findings support the hypothesis that continuous selection has reduced the toxicity of EE2 and depleted genetic variance for tolerance to this synthetic stressor.

show abstract

Influence of short-term exposure to low levels of 17α-ethynylestradiol on expression of genes involved in immunity and on immune parameters in rainbow trout, Oncorhynchus mykiss

Cited by 22 publications

References 86 publications

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis : Investigation of immune-related parameters

Low adaptive potential for tolerance to ethynylestradiol, but also low toxicity, in a grayling population (Thymallus thymallus)

Low adaptive potential for tolerance to ethynylestradiol, but also low toxicity, in a grayling population (Thymallus thymallus)

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