SUMMARYWhen used to restrain rats while obtaining cardiac blood, there was little difference between the effects of ether, pentobarbitone sodium, and fentanyl plus droperidol on acid-base balance, haemoglobin, haematocrit, plasma protein, calcium and magnesium. The use of manual restraint increased blood acidity, plasma protein, calcium and magnesium, but decreased blood glucose. Haemoglobin and haematocrit values were higher for tail blood than for blood removed from the heart or abdominal aorta.
85The removal of blood from the rat usually involves a certain degree of stress to the animal.During work on iron metabolism in progress in this laboratory a number of blood sampling methods were used, with varying degrees of response from the rat. Thus it was decided to compare the effects of these methods of blood sampling on some widely used biochemical and haematological parameters. The effects of restraining rats using a gaseous anaesthetic (ether), an intraperitoneal anaesthetic (pentobarbitone sodium), and a neuroleptanalgesic agent (a combination of fentanyl and droperidol), were compared with manual restraint.In another experiment the influence of the site of blood removal on some acid-base and haematological values was examined.
MATERIALS AND METHODSFemale albino Wi star rats of about 300 g bodyweight were used in this study and were maintained on a balanced pelleted diet prior to the experiments.
Effects of restraintThe following methods of restraint were employed while cardiac punctures were carried out to remove blood. ·Present address: