Influence of serotonin on progesterone and estradiol secretion of cultured human granulosa cells

Bódis, József; Török, Á.; Tinneberg, Hans‐Rudolf; Hanf, Volker; Hamori, M.; Cledon, P.

doi:10.1016/s0015-0282(16)55017-1

Cited by 40 publications

(22 citation statements)

References 9 publications

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“…Other studies [8] showed that preovulatory human FF contains noradrenaline, dopamine and serotonin. In an in vitro cell culture system, serotonin exhibited a significant stimulatory effect on PG secretion by human GCs [2]. The observed effect was dose-related and could be inhibited by the ß-receptor antagonist propranolol.…”

Section: Discussionmentioning

confidence: 92%

“…It was first reported by Webley et al [1] that human granulosa cells (GCs) in serum-supplemented medium are capable of responding to melatonin (Me) stimulation. Bó dis et al [2] found that serotonin enhances progesterone (PG) and estradiol (E 2 ) secretion by human GCs cultured in serum-free medium. Papenfuss et al [3] reported the modulatory effect of catecholamines on gonadotropin-stimulated PG and E 2 secretion of GCs.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Bódis

Koppán

Kornya

et al. 2001

Gynecol Obstet Invest

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The aim of this study was to explore the direct action of melatonin (Me) on basal and gonadotropin-stimulated progesterone (PG) and estradiol (E₂) secretion of human granulosa cells (GCs) cultured in serum-free medium and in a superfused GC system. Human GCs were isolated from preovulatory follicular fluid aspirated from 34 women undergoing in vitro fertilization at the University Women’s Hospital of Tübingen. PG and E₂ production was measured in the presence and absence of Me, propranolol, LH or FSH using radioimmunoassay. Statistical analysis of the data was performed by ANOVA and Newman-Keuls test. Me stimulated E₂ secretion in a dose-dependent manner. Propranolol did not cause any change in E₂ secretion, and when given with Me, it only partially blocked but could not entirely prevent E₂ output. There was no statistically significant effect of Me on PG production when Me was administered at concentrations between 10^–4 and 10^–8 M. However, at 10^–3 M Me significantly suppressed PG output of granulosa cells. LH and FSH significantly stimulated the secretion of both steroid hormones. Me significantly reduced LH- and FSH-induced E₂ secretion, as well as LH-stimulated PG output, while it caused only a slight, yet significant decrease in PG secretion. In the superfused GC system, FSH and LH resulted in a significant stimulatory effect on PG release. Me did not modify the stimulatory effect of FSH on PG, while it caused some delay in LH-stimulated PG release. Propranolol and Me had no stimulatory effect on PG release. On the basis of our results we suggest that Me has a direct modulatory effect on basal E₂ and gonadotropin-stimulated E₂ and PG secretion of human GCs. The observed effect may play a physiological role in the regulation of GC function during the menstrual cycle.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Bódis

Koppán

Kornya

et al. 2001

Gynecol Obstet Invest

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“…As regards granulosa cells in humans, there are reports claiming that serotonin can release (Bodis et al, 1992) or inhibit (Schaeffer and Sirotkin, 1995) progesterone and estradiol from human granulosa cells in culture. Figure 2 shows the effect of serotonin applied to the celiac ganglion in an in vitro Ganglion-SON-Ovary integrated system on the ovarian production of progesterone in animals in DI and DII.…”

Section: Innervation and Ovarian Functionmentioning

confidence: 99%

Role of the central and peripheral nervous system in the ovarian function

Aguado

2002

Microscopy Res & Technique

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This review attempts to give a comprehensive overview of ovarian innervation, considering the whole nervous system and its different levels that may modify the ovarian function. The connection between the ovary and the central nervous system through the autonomic pathways, including the peripheral ganglia, is highlighted. The evidence obtained over the last years highlights the role of the superior ovarian nerve (SON) in the ovarian phenomena. Besides, the effect on the ovary of conventional neurotransmitters and others such as indolamines and peptides, which have been found in this organ, are discussed. Various reproductive diseases have been studied almost exclusively from the endocrine point of view. It is evident that a better knowledge about the role of the neural factors involved in the ovarian physiology may facilitate the understanding of some of these. A review of the concepts and an update of some experimental designs is made that permits clarifying several aspects of the relationship between the neural system and the ovary. At present, there is no doubt that the innervation of the ovary is involved in several physiological aspects of this gland function. However, the relationship of some levels of the nervous system and the ovary offer a wide avenue for future research.

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“…Thus, in bivalve mollusks, 5-I-rI~ acting via receptors located on oocyte surfaces, initiates maturation divisions, and a number of 5 -H T antagonists specifically block meiosis [36,43,44,48,56,57]. In starfish and vertebrates, neurotransmitters function as modulators of maturation hormones: 1-methyladenine or hypophyseal hormones and progesterone respectively [3,4,9,16,17,24,31,76]. In starfish, 5-HT potentiates the action of 1-methyladenine, and a variety of 5-HT antagonists specifically and reversibly inhibit oocyte maturation induced by 1-methyladenine.…”

Section: Neuronal and Pre-nervous Neurotransmitters As Triggers And Rmentioning

confidence: 99%

Changes in the physiological roles of neurotransmitters during individual development

Buznikov

Shmukler

Lauder

1999

Neurosci Behav Physiol

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The classical neurotransmitters (acetylcholine and biogenic monoamines) are multifunctional substances involved in intra- and intercellular signaling at all stages of ontogenesis in multicellular animals. A cyclical scheme is proposed to describe age-related changes in neurotransmitter functions at different stages of development from oocyte maturation to neuron formation. This may reflect not only the temporospatial organization of neurotransmitter processes, but also the origin of the functions of acetylcholine and biogenic monoamines from the protosynapses of the cleaved embryo to neuronal synapses.

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Influence of serotonin on progesterone and estradiol secretion of cultured human granulosa cells

Cited by 40 publications

References 9 publications

Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Role of the central and peripheral nervous system in the ovarian function

Changes in the physiological roles of neurotransmitters during individual development

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