Influence of seminal plasma, spermatozoa and semen extender on cytokine expression in the porcine endometrium after insemination

Jiwakanon, Jatesada; Persson, Erik; Berg, Mikael; Dalin, Anne-Marie

doi:10.1016/j.anireprosci.2010.11.016

Cited by 28 publications

(62 citation statements)

References 30 publications

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“…These findings may indicate the presence of immune-stimulating constituents in the artificial semen extender and the lack of such in SP. Therefore, Taylor, et al [36] suggested the existence of immune suppressing substances in porcine SP and this hypothesis is recently supported by findings of Jiwakanon, et al [7]. Quite similar concept about the protection of spermatozoa from harmful influences was offered by Aloe, et al [37] who have shown the ability of bovine SP and vesicular gland fluid (VGF) to block IL-8 stimulated PMN transmigration in vitro and to reduce the incidence of cell-damageing reactive oxygen species.…”

Section: Discussionmentioning

confidence: 84%

“…Human SP stimulated mRNA expression of IL-6 in endometrial epithelial cells without any effect on TNFα expression [43] and induced an increase of IL-6 and IL-8 secretion by peripheral mononuclear blood cells whereas the secretion of TNFα was reduced [44]. In gilts, immunohistochemical labelling for IL-6 in the endometrium was slightly higher 35 -40 h than 5 -6 h after insemination [7]. The gene responsible for the IL-6 receptor was up-regulated in the endometrium of pregnant compared to non-pregnant sows [45].…”

Section: Discussionmentioning

confidence: 91%

“…These two prostaglandins are also involved in acute and chronic inflammatory conditions [4,5]. Seminal plasma suppresses the migration of polymorphonuclear neutrophil granulocytes (PMNs) into the uterus, decreases PMNs infiltration of the endometrium and enhances the rate of disappearance of uterine inflammation in gilts following breeding [6,7]. Rozeboom, et al [8] suggested that non-estrogenic factors in seminal plasma may protect spermatozoa from an inflamed uterine environment and consequently a suitable population of viable spermatozoa is able to reach the site of fertilization.…”

Section: Introductionmentioning

confidence: 99%

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Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

Madej¹,

Hansen²,

Johannisson³

et al. 2013

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The objectives of this study were to explore whether heparin-binding proteins, separated by fast protein liquid chromatography from boar seminal plasma influence the release of prostaglandins F_2α, (PGF_2α), E₂ (PGE₂) and interleukin-6 (IL-6) by porcine endometrial and cervical cells and even bovine endometrial cells. In Experiment I, we showed that release of PGF_2α by endometrial epithelial, endometrial stromal and cervical stromal cells to the medium was inhibited (p < 0.05) to 9.0% - 60.6% after 24 h incubation with 125 μg of heparin-binding proteins. Tumor necrosis factor α (TNFα) stimulated release of IL-6 by endometrial and cervical stromal cells after 24 h incubation, but in the presence of heparin-binding proteins, this stimulation was attenuated. Release of PGF_2α by cryopreserved (Experiment II) and primary (Experiment III) cervical stromal cells was significantly inhibited after 3 h incubation with 66 - 95.4 μg of heparin- binding proteins. A significant inhibition of PGE₂ release by cryopreserved and primary cervical stromal cells was already achieved after incubation with 16.5 - 23.9 μg of heparin-binding proteins. The release of IL-6 by cryopreserved cells was stimulated after 3 h incubation with heparin- binding proteins in a dose dependent manner in contrast to the release of IL-6 by freshly isolated cervical stromal cells. We also found (Experiment IV) that porcine heparin-binding seminal plasma proteins inhibited release of PGF_2α and stimulated release of IL-6 by bovine endometrial epithelial cells. In conclusion, a group of heparin-binding proteins separated by fast protein liquid chromatography

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

Madej¹,

Hansen²,

Johannisson³

et al. 2013

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“…We have previously studied the effects of SP on the cytokines TGF-β1, IL-6 and IL-10 in different parts of the oviduct [17] and endometrium [18] both shortly after insemination (5–6 h) and later (35–40 h). The results showed that at 35–40 h after infusion, not only SP but also semen extender (BTS) and spermatozoa in BTS, up-regulated TGF-β1 mRNA expression compared with the control (catheter insertion) in the oviduct (isthmus and infundibulum).…”

Section: Introductionmentioning

confidence: 99%

“…The mRNA expression of IL-10 and IL-6 in both the oviduct and the endometrium were significantly lower than for TGF-β1. IHC-labeling of TGF-β1, IL-10 and IL-6, was evident, especially in the epithelial cells of both the oviduct [17] and the endometrium [18]. …”

Section: Introductionmentioning

confidence: 99%

Seminal plasma did not influence the presence of transforming growth factor-β1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination

2013

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BackgroundThe effects of seminal plasma on the presence of the cytokines transforming growth factor (TGF)-β1, interleukin (IL)-10 and IL-6 in ovarian follicles and follicular fluid were studied shortly after insemination in gilts.Ovaries from gilts were sampled 5–6 h after insemination with either seminal plasma (SP), fresh semen in extender (Beltsville thawing solution, BTS), spermatozoa in extender (Spz), or only BTS (control).ResultsImmunohistochemical (IHC) labeling of TGF-β1, IL-10 and IL-6 was evident in the ovarian oocytes and granulosa cells independent of stage of follicular development (antral follicles). Theca interna cells were labeled to a high degree in mature follicles. No consistent differences between treatment groups could be observed for any of the cytokines.In follicular fluid, high concentrations of TGF-β1 were found while the levels of IL-10 and IL-6 were low. There were no differences between treatment groups.ConclusionsOur results show a presence of the cytokines TGF-β1, IL-6 and IL-10 in oocytes, granulosa and theca cells, as well as in the fluid of mature follicles suggesting a role of these cytokines in intra-ovarian cell communication. However, treatment (SP, fresh semen in BTS, spermatozoa in BTS or BTS) did not influence the IHC-labeling pattern or the levels of these cytokines in follicular fluid shortly after insemination.

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Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

Jalali

Kitewska

Wasielak

et al. 2014

Molecular Reproduction Devel

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Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune changes in leukocyte populations were measured by flow cytometry and mRNAs for various cytokines by quantitative reverse-transcriptase PCR in porcine endometrium collected on Days 6 and 13 from cycling and pregnant animals or from animals given seminal plasma infusions. Seminal plasma infusion induced long-term modulatory effects, resulting in significantly more endometrial FoxP3-positive T-regulatory and T-helper cells 6 days after infusion as compared to cycling and pregnant animals. The number of T-cytotoxic and T-null cells did not change between the studied groups. The early molecular effects of seminal plasma were not observed at 13-days post-infusion, although animals on Day 13 of pregnancy did show significantly more T-cells (of any type investigated). Seminal plasma also showed a delayed effect on cytokine expression, specifically exhibiting a significant increase in interleukin 10 (IL10) and a decrease in granulocyte macrophage colony-stimulating factor (GMCSF) gene expression on Day 13 as compared to Day 6 of cycling or pregnant gilts. The results indicate a delayed regulatory effect of seminal plasma on immune responses in the porcine uterus, which are similar to immune changes generated by implanting conceptuses.

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Influence of seminal plasma, spermatozoa and semen extender on cytokine expression in the porcine endometrium after insemination

Cited by 28 publications

References 30 publications

Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

Seminal plasma did not influence the presence of transforming growth factor-β1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination

Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

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