Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

Madej, Malgorzata; Hansen, C. F.; Johannisson, Anders; Madej, Andrzej

doi:10.4236/ns.2013.57a004

Cited by 4 publications
(4 citation statements)

References 45 publications

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“…Furthermore, it can stimulate IL‐6 release but decrease the level of PGF 2α by bovine endometrial and cervical cells. These responses are involved in the inflammatory process (Madej, Hansen, Johannisson, & Madej, ). Previous studies showed that the composition of bovine SP may vary with fertility; the SP from high‐fertility bulls contained two major proteins (26 kDa, pI 6.2; 55 kDa, pI 4.5) with a higher density than in bulls of average fertility and below average fertility (Killian et al., ).…”

Section: Discussionmentioning
confidence: 99%

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture
Nongbua
¹
,
Guo
²
,
Edman³
et al. 2017
Reprod Domestic Animals
11
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3
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The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0-13 × 10 cells per flask, were challenged with SP from bulls of high or low fertility (n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr (n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high- or low-fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high- or low-fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose-dependent and fertility-dependent.

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Section: Discussionmentioning
confidence: 99%

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture
Nongbua
¹
,
Guo
²
,
Edman³
et al. 2017
Reprod Domestic Animals
11
0
3
0
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“…It has been shown that seminal plasma is important for boar semen fertility in vitro (Maxwell et al 2007;Wolf and Smital 2009;Nasrin and Calogero 2012;Stancic et al 2012b), as well as in vivo owing to its role in promoting transport, survival and fertilisation in the female reproductive tract (Langedijk et al 2002;Strzezek et al 2005;Waberski et al 2006;Madej et al 2013). Overextended seminal plasma reduces the progressive motility of spermatozoa and increases the number of spermatozoa with damaged acrosomes or disintegrated acrosomal membranes (Kommisrud et al 2002;Maxwell et al 2007;Stancic et al 2012a;Chutia et al 2014).…”

Section: Discussionmentioning
confidence: 99%

Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days
Apić¹,
Stančić²,
Vakanjac³
et al. 2016
Vet. Med. - Czech
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ABSTRACT:The effect of adding high or low protein content homologous seminal plasma to boar spermatozoa on the progressive motility of the spermatozoa after storage for three days, and at a 1 : 4 dilution ratio was investigated. A total of 32 ejaculates collected from four boars (eight ejaculates per boar) with high seminal plasma protein content (4%, HH-group) and 32 ejaculates collected from four boars (eight ejaculates per boar) with low seminal plasma protein content (2%, LL-group) were evaluated. The fresh ejaculate samples were centrifuged at 1000 g for 10 min at 4 °C to separate the spermatozoa from the seminal plasma. After centrifugation, one of the centrifuged spermatozoa samples was used to form an autologous fresh ejaculate sample, and another to form a homologous sample by adding low or high protein content seminal plasma from other boars. It was found that semen samples formed with spermatozoa from HH-group boars, supplemented with seminal plasma from LL-group, boars have significantly (P < 0.01) lower progressive motility (55%) after storage for 72 h than samples containing the boar's own (autologous) seminal plasma (65%). Conversely, when homologous seminal plasma with high protein content was added to the spermatozoa isolated from the boar ejaculate with low protein content in its seminal plasma, progressive motility significantly (P < 0.01) increased from 52% in samples with autologous seminal plasma, to 65% in samples with homologous seminal plasma. It was concluded that addition of homologous high protein content seminal plasma to the spermatozoa of boars with low protein content in their seminal plasma increases their progressive motility after storage for 72 h at a 1 : 4 dilution ratio. This could be a useful tool for increasing reproductive performance in lower fertility high genetic quality boars.

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“…The SP was filtered into 4 mL tubes using a membrane syringe filter of 0.2 µm size, frozen at -80ºC, transported to Sweden, and stored until analyzed by affinity liquid chromatography and assessment of testosterone concentration. (mAU*mL), and percentage of area for each peak in relation to the total area were recorded for each fraction as well as the ratios of the different fractions [16,17].…”

Section: Preparation Of Seminal Plasmamentioning
confidence: 99%

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins
Johannisson
¹
,
Al-Essawe
²
,
Al-Saffar
³

et al. 2020
Journal of Reproduction and Development
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4
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The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer stallions. Only minor variations in the major protein groups were found between the breeding and non-breeding seasons. In the non-breeding season, a higher content of a subset of non-heparin binding proteins as well as of heparin-binding proteins was found. Analysis of semen characteristics revealed a somewhat contrasting picture. While only minor variations in sperm kinematics and sperm morphology were found between seasons, the flow-cytometric measurements of mitochondrial membrane potential and also, to some extent, reactive oxygen species production indicated lower sperm quality in the breeding season. Chromatin integrity and testosterone levels were unchanged between seasons. The results suggest that stallion ejaculates could be used year-round for freezing, since only minor differences in protein composition exist between the breeding and non-breeding seasons, as well as between good and bad freezers. In addition, sperm quality is not impaired during the non-breeding season.

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Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

Abstract:
The objectives of this study were to explore whether heparin-binding proteins, separated by fast protein liquid chromatography from boar seminal plasma influence the release of prostaglandins F_2α, (PGF_{2α Show more}

Cited by 4 publications

References 45 publications

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture

Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

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Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

Abstract: The objectives of this study were to explore whether heparin-binding proteins, separated by fast protein liquid chromatography from boar seminal plasma influence the release of prostaglandins F2α, (PGF2α Show more

Cited by 4 publications

References 45 publications

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture

Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

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Product

Resources

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Abstract:
The objectives of this study were to explore whether heparin-binding proteins, separated by fast protein liquid chromatography from boar seminal plasma influence the release of prostaglandins F_2α, (PGF_{2α Show more}