Influence of Semen Collector on Semen Characteristics of Murrah Buffalo and Crossbred Bulls

Abstract

| A total of 156 ejaculates each from 8 Murrah buffalo and 8 Crossbred (Karan Fries) bulls were collected during one year period at Artificial Breeding Research Centre, NDRI, Karnal, India and analysed using least squares analysis to quantify the effect of collector on various semen quality parameters. The overall least squares mean values of Murrah buffalo and Crossbred bulls for ejaculate volume (ml), mass activity, Initial motility (%), sperm concentration (10 6 /ml), total sperm output (10 6 ), non-eosinophilic count (%), HOST (%), acrosome integrity (%), head abnormality (%), mid-piece abnormality (%), tail abnormality (%), total abnormality (%), pH and osmolality (mOsmol/kg) were 2.66±0. Collector was found to have significant (p < 0.01) influence on all semen quality traits of Murrah buffalo bulls, but there were no significant differences (p<0.05) between collector 1 and collector 2. In case of Karan Fries bulls, the collector had significant (P<0.05) effect on ejaculate volume (4.84 vs. 4.06 ml) and total sperm output (4032.19 vs. 3346.19 10 6 ), but did not have significant (P<0.05) effect on other semen quality parameters. It was concluded that semen collection is highly skilled and scientific job and need efficient collector to achieve collection of optimum quantity and quality of semen, which is the major aim of semen stations. Keywords

Effect of egg yolk powder on freezability of Murrah buffalo (Bubalus bubalis) semen

“…The percentage of live spermatozoa was higher than the values reported by Bhakat et al . [ 14 ]. No significant differences in viability percentage were recorded among any groups post-dilution.…”

“…The percentage of abnormal spermatozoa was in agreement to the reports of Bhakat et al . [ 14 ]. No significant difference in the percentage of abnormal spermatozoa was recorded after dilution and at the pre-freeze stage.…”

“…[ 15 ] but higher than the values reported by Bhakat et al . [ 14 ]. No significant difference in HOST response was recorded after dilution among any groups.…”

Serum kisspeptin: New possible biomarker for sexual behaviour and sperm concentration in buffalo bulls

“…The minimum and maximum ejaculate volumes of buffalo bulls observed in the study were 0.6 and 9.0 ml, respectively. Several reports have shown that semen ejaculate volume of Murrah buffalo bulls ranged between 2.78 and 5.0 ml (Bhakat, Mohanty, Raina, Gupta, & Khan, 2011; Bhakat et al., 2015; Henry et al., 2017). In this study, aggressiveness, penile erection and reaction time were not correlated with semen volume.…”

“…Libido and mating ability are essential parameters needed for selection of breeding males of all domestic species, including buffalo (Anzar, Ahmad, Nazir, Ahmad, & Shah, 1993; Chenoweth, 1981). Sexual behaviour of breeding bulls during natural mating as well as during semen collection (Purohit, Singh, Vyas, & Yadav, 1998; Samo, Brohi, Kaka, Qureshi, & Memon, 2005; Singh et al., 2015) has been investigated over a large male population; but such studies in buffalo bulls have been conducted over small sample size (Anzar et al., 1993; Bhakat et al., 2015; Dixit, Agarwal, Agarwal, & Dwaraknath, 1984; Mandal, Nagpaul, & Gupta, 2000; Singh, Sengupta, & Tripathi, 2001). Since, poor libido as well as poor semen quality contribute to the major culling factors in cattle and buffalo males (Kumar, Singh, Dhaliwal, & Singh, 2008; Mukhopadhyay et al., 2011).…”