Effect of egg yolk powder on freezability of Murrah buffalo (Bubalus bubalis) semen

Abstract

Aim:The aim of this study was to investigate the effect of commercial egg yolk powder as an alternative to fresh egg yolk on freezability of Murrah buffalo semen.Materials and Methods:Semen samples (12) from 3 Murrah buffaloes (4 from each bull) with mass motility (≥3+) and total motility (70% and above) were utilized in this study. Immediately after collection, each sample was divided into four groups. Groups I was diluted up to 60×106 sperm/ml with tris extender containing 10% fresh egg yolk and Groups II, III, and IV were diluted up to 60×106 sperm/ml with tris extender containing 2%, 4%, and 6% egg yolk powder, respectively. Semen samples were processed and cryopreserved followed by examination of frozen semen samples after 24 h. Semen samples from each group were evaluated for total motility, viability, acrosomal integrity, abnormality, and hypo-osmotic swelling test (HOST) response after dilution, pre-freeze, and post-thaw stage.Results:Pre-freeze total motility was significantly (p<0.05) higher in Groups III and IV as compared to Groups I and II, and post-thaw total motility was significantly (p<0.01) higher in Group III as compared to other three groups. Viability was significantly (p<0.05) higher in Groups II, III, and IV than Group I at the pre-freeze stage. Significantly (p<0.01) higher viability and acrosomal integrity were recorded in Group III as compared to other three groups at the post-thaw stage. Abnormality was significantly (p<0.05) higher in Group IV than other three groups. HOST response was significantly (p<0.05) higher in Groups II and III than Groups I and IV at the pre-freeze and post-thaw stages.Conclusion:Addition of egg yolk powder at 4% level yielded significantly better results in terms of post-thaw semen quality as compared to the fresh egg yolk and other concentrations of egg yolk powder (2% and 6%).

Effects of selective extenders on buffalo semen quality at physical and molecular level

“…The findings of the present study are in agreement with Meena et al (2010) who also found higher sperm motility and viability in Tris-egg yolk based extender than Soybean based extender. Pre-freeze total and progressive motility and post-thaw total and progressive motility in case of tris-egg yolk extender are comparable with the findings of Akhter et al (2011), Sing et al (2013, Chaudhari et al (2015), and Kumar et al (2016). The findings of this study regarding motility parameters are also comparable with the reports of Singh et al (2012), Singh et al (2013) and Rehman et al (2014).…”

Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen–Thawed Rooster Sperm?

“…The present study further demonstrates that a suitable concentration for the addition of MitoQ (between 50 and 150 nM) can improve the mitochondrial damage caused by cryopreservation. Furthermore, the acrosomal integrity of spermatozoa is critical for the sperm’s fertilization ability, which is closely associated with capacitation, a normal acrosome reaction [ 30 ]. The present study showed that positive effects of MitoQ were observed in the acrosome’s integrity with respect to thawed sperm.…”