Influence of secondary colonizers and human plasma on the adherence of <i>Porphyromonas gingivalis in vitro</i>

Satô, Masaru; Nagayama, Motohiko; Yamaguchi, Ryosuke; Fujiwara, Shuu; Takeuchi, Hiroshi

doi:10.1034/j.1600-0447.2002.201224.x

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…For S. mitis BMS blocking of attachment by biosurfactant production has been reported (29), which might be unique to S. mitis among the current collection of antagonistic strains and could be more effective than the interference mechanisms described hitherto for S. sanguinis , S. crista , and S. salivarius (7, 27, 32). Interestingly, previous reports showed, instead of antagonistic effects, synergistic effects of A. naeslundii on P. gingivalis through co‐aggregation (33) and enhancement of P. gingivalis adhesion (20). However, because it is clinically known that Actinomyces occurs more in healthy pockets than in diseased pockets (12), we believe that the A. naeslundii strain studied here must be considered as an antagonist of P. gingivalis .…”

Section: Discussionmentioning

confidence: 98%

Reduction of periodontal pathogens adhesion by antagonistic strains

Hoogmoed

Geertsema-Doornbusch

Teughels

et al. 2007

Oral Microbiology and Immunology

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Section: Discussionmentioning

confidence: 98%

Reduction of periodontal pathogens adhesion by antagonistic strains

Hoogmoed

Geertsema-Doornbusch

Teughels

et al. 2007

Oral Microbiology and Immunology

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“…It requires specific pioneer colonizers (e.g., Streptococcus gordonii and Veillonella sp.) or secondary colonizers (e.g., Fusobacterium nucleatum ) to attach to a surface or to grow in a biofilm ( 31 , 33 ). We also grew the P. gingivalis strain ATCC 33277 alone under the same experimental condition (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Manipulation of Saliva-Derived Microcosm Biofilms To Resemble Dysbiotic Subgingival Microbiota

Jiang

Brandt

Buijs

et al. 2021

Appl Environ Microbiol

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Periodontitis is a highly prevalent oral inflammatory disease triggered by dysbiotic subgingival microbiota. For the development of microbiome modulators that can reverse the dysbiotic state and re-establish a health-related microbiota, a high-throughput in vitro multi-species biofilm model is needed. Our aim is to establish a model that resembles a dysbiotic subgingival microbial biofilm by incorporating the major periodontal pathogen Porphyromonas gingivalis into microcosm biofilms cultured from pooled saliva of healthy volunteers. The biofilms were grown for 3, 7, and 10 days and analyzed for their microbial composition by 16S rDNA amplicon sequencing as well as dipeptidyl peptidase IV (DPP4) activity and butyric acid production. The addition of P. gingivalis increased its abundance in saliva-derived microcosm biofilms from 2.7% on day 3 to >50% on day 10, which significantly reduced the Shannon diversity, but did not affect the total number of operational taxonomic units (OTUs). The P. gingivalis-enriched biofilms displayed altered microbial composition as revealed by principle component analysis and reduced interactions among microbial species. Moreover, these biofilms exhibited enhanced DPP4 activity and butyric acid production. In conclusion, by adding P. gingivalis into saliva-derived microcosm biofilms, we established an in vitro pathogen-enriched dysbiotic microbiota, which resembles periodontitis-associated subgingival microbiota in terms of increased P. gingivalis abundance and higher DPP4 activity and butyric acid production. This model may allow for investigating factors that accelerate or hinder microbial shift from symbiosis to dysbiosis and for developing microbiome modulation strategies. IMPORTANCE In line with the new paradigm of the etiology of periodontitis, an inflammatory disorder initiated by dysbiotic subgingival microbiota, novel therapeutic strategies have been proposed, targeting reversing dysbiosis and restoring host-compatible microbiota, rather than eliminating the biofilms unselectively. Thus, appropriate laboratory models are required to evaluate the efficacy of potential microbiome modulators. In the present study, we used the easily obtainable saliva as an inoculum, spiked the microcosm biofilms with the periodontal pathogen Porphyromonas gingivalis, and obtained a P. gingivalis-enriched microbiota, which resembles the in vivo pathogen-enriched subgingival microbiota in severe periodontitis. This biofilm model circumvents the difficulties encountered when using subgingival plaque as the inoculum and achieves microbiota in dysbiotic state in a controlled and reproducible manner, which is required for high-throughput and large scale evaluation of strategies that can potentially modulate microbial ecology.

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“…Indeed, blocking is determined by repulsive interactions between the adhering antagonist and the periodontopathogen and larger blocking effects may arise, for instance, from biosurfactant production (22). Reports have also shown, instead of antagonistic effects, synergistic effects of A. naeslundii on P. gingivalis through co-aggregation (23) and enhancement of P. gingivalis adhesion (24). However, because it is clinically known that Actinomyces occurs more in healthy pockets than in diseased pockets (25), we believe that the A. naeslundii strain studied here may be considered as an antagonist of P. gingivalis.…”

Section: Prevalence Of Beneficial Bacteria In Aggressive Periodontitismentioning

confidence: 99%

An Insight into the Role of Benefical Bacteria in Periodontal Pocket Recolonization: A Literature Review

Sm¹,

Suchetha²,

Mundinamane³

et al. 2014

Journal of Oral Health and Community Dentistry

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Microflora can be found in both caries-free and periodontitis-free people and caries-affected and periodontitisaffected people, and many clinical studies reveal that the portion of certain bacterial species such as Streptococcus mutans or Porphyromonas gingivalis, respectively, is increased in patients with caries or periodontitis. Therefore, it seems that the competition that results between beneficial bacteria and virulent bacteria leads to either a healthy or sick status of human beings. Competition between members of the dental microflora and there role in pocket recolonization is very complex and many antagonistic characteristics can be observed from competition for initial attachment on tooth surfaces or for later attachment to pioneer bacteria, competition from bacteriocins or hydrogen peroxide secreted and from facilitating the growth of some species which inhibit other species. To date only some of the details of these mechanisms are known. The present review will provide an overview on the prevalence of beneficial bacteria and the major mechanisms of oral bacterial interactions. Due to the large number of oral bacterial species, only the best characterized species are included in this review.

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Influence of secondary colonizers and human plasma on the adherence of Porphyromonas gingivalis in vitro

Cited by 4 publications

References 38 publications

Reduction of periodontal pathogens adhesion by antagonistic strains

Reduction of periodontal pathogens adhesion by antagonistic strains

Manipulation of Saliva-Derived Microcosm Biofilms To Resemble Dysbiotic Subgingival Microbiota

An Insight into the Role of Benefical Bacteria in Periodontal Pocket Recolonization: A Literature Review

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