Influence of sample application mode on performance of pressurized planar electrochromatography in completely closed system

High performance stationary phases for planar chromatography

“…The plate height generally declines with increasing migration distance reaching a plateau or shallow region between about 35 and 75 mm [57,61,63,64]. The lowest observed plate heights for the plateau region fall into the range 10-15 m corresponding to H ≈ 2d p for high performance pre-coated octadecylsiloxane-bonded silica layers.…”

“…There are specific problems associated with sample application since the layer must be completely impregnated with the mobile phase at the start of the separation while samples are typically applied to the dry layer [57,63]. In addition, sample zones applied to the layer are generally too large to obtain optimum performance and cited plate height measurements are usually calculated by subtraction of the variance due to the sample application zone.…”

Separation of Some Aromatic Amino Acid Enantiomers with Pressurized Planar Electrochromatography and TLC

“…PPEC is relatively new mode of separation in which the mobile phase is driven into movement by electroosmotic effect in closed separation system [13][14][15][16][17][18][19][20][21][22][23][24][25]. This movement can be easily controlled by electric field strength and mobile phase composition (pH of the mobile phase, buffer concentration, organic solvent type and its concentration) [13][14][15][16][17][18][19][20].…”

“…This movement can be easily controlled by electric field strength and mobile phase composition (pH of the mobile phase, buffer concentration, organic solvent type and its concentration) [13][14][15][16][17][18][19][20].…”

“…The development of PPEC has been practically reported by three research groups [13][14][15][16][17][18][19][20][21][22][23][24][25] so far. In these papers, few distinct features of PPEC such as high efficiency, short time of separation process and different separation selectivity have been presented relative to thin-layer chromatography/high-performance thin-layer chromatography (TLC/HPTLC).…”