Influence of Salt Stress on Growth of Spermosphere Bacterial Communities in Different Peanut (Arachis hypogaea L.) Cultivars

Abstract

Background: Exposure of seeds to high salinity can cause reduced germination and poor seedling establishment. Improving the salt tolerance of peanut (Arachis hypogaea L.) seeds during germination is an important breeding goal of the peanut industry. Bacterial communities in the spermosphere soils may be of special importance to seed germination under salt stress, whereas extant results in oilseed crop peanut are scarce. Methods: Here, bacterial communities colonizing peanut seeds with salt stress were characterized using 16S rRNA gene sequencing. Results: Peanut spermosphere was composed of four dominant genera: Bacillus, Massilia, Pseudarthrobacter, and Sphingomonas. Comparisons of bacterial community structure revealed that the beneficial bacteria (Bacillus), which can produce specific phosphatases to sequentially mineralize organic phosphorus into inorganic phosphorus, occurred in relatively higher abundance in salt-treated spermosphere soils. Further soil enzyme activity assays showed that phosphatase activity increased in salt-treated spermosphere soils, which may be associated with the shift of Bacillus. Conclusion: This study will form the foundation for future improvement of salt tolerance of peanuts at the seed germination stage via modification of the soil microbes.

Comparative metagenomic analysis reveals rhizosphere microbial community composition and functions help protect grapevines against salt stress

“…The taxonomic profiling results demonstrated that the rhizosphere microbiota of the two grapevine varieties shared the same 10 dominant phyla ( Figure 1D ). All of the identified phyla have been previously reported and are known to predominate grapevine rhizospheres ( Zhang et al, 2019 ), as well as in those of peanut ( Xu et al, 2020 ), pomegranate ( Ravinath et al, 2022 ), barley ( Bulgarelli et al, 2015 ), and Arabidopsis thaliana ( Kielak et al, 2016 ). Nevertheless, the relative abundances of these phyla were distinct between 101-14 and 5BB ( Figures 1E , F ; Supplementary Table 2 ).…”

Genome-wide analysis of the peanut CaM/CML gene family reveals that the AhCML69 gene is associated with resistance to Ralstonia solanacearum

“…As an important oil crop and protein source, peanut plants can be grown in poor-quality soil [ 45 , 46 ], but their yield is significantly restricted by BW caused by R. solanacearum [ 27 ]. However, studies on the mechanisms of the peanut- R. solanacearum interaction are rare, and no R genes conferring resistance to BW have been identified in peanut.…”

Green manure increases peanut production by shaping the rhizosphere bacterial community and regulating soil metabolites under continuous peanut production systems

“…The soil organic carbon was measured by humid oxidation with potassium dichromate (K 2 Cr 2 O 7 ), available nitrogen was extracted by using the alkaline hydrolysis diffusion method, and available phosphorus was determined by sodium bicarbonate (NaHCO 3 ) spectrophotometry [ 7 ]. Soil enzyme activities including invertase, urease, and neutral phosphatase were measured according to the previous study [ 3 ]. Soil invertase activity was determined using the 3, 5-dinitrosalicylic acid colorimetric spectrophotometry (absorbance at 508 nm); whereas urease activity was determined using sodium phenolate and sodium hypochlorite spectrophotometry (absorbance at 578 nm); soil neutral phosphatase activity was examined by using Solarbio Soil Neutral Phosphatase (S-NP) Activity Assay Kit (BC0465) [ 3 ].…”

“…Soil enzyme activities including invertase, urease, and neutral phosphatase were measured according to the previous study [ 3 ]. Soil invertase activity was determined using the 3, 5-dinitrosalicylic acid colorimetric spectrophotometry (absorbance at 508 nm); whereas urease activity was determined using sodium phenolate and sodium hypochlorite spectrophotometry (absorbance at 578 nm); soil neutral phosphatase activity was examined by using Solarbio Soil Neutral Phosphatase (S-NP) Activity Assay Kit (BC0465) [ 3 ].…”