Influence of Salicylic Acid on Production Characteristics and Biochemical Parameters of Tetraselmis Suecica in Enrichment Culture

Abstract

The article considers the use of salicylic acid as a stimulator of microalgae growth. The influence of a wide range of salicylic acid concentrations on the growth dynamics of Tetra-selmis suecica in enrichment culture has been evaluated. Cultivation was carried out in monoculture. An increase in algal biomass was measured by the increasing number of cells counted in each experiment in three Goryaev chambers under a light microscope. The duration of the experiments was 14 days. It is shown that salicylic acid in concentrations of 0.4-1.9 • 10–5 M inhibits the growth of algae. The growth of the control culture has two pronounced peak numbers on 4th and 12th days of the experiment. The introduction of salicylic acid in concentrations of 0.44-1.9 • 10–5 M was accompanied by a change of the growth curves: the maximum number of cells was observed on 12th day of the experiment. A higher concentration of phytohormone (3.75.• 10–5 M) provided an increase in crop density by 414% over 14 days of the experiment. The growth of T. suecica culture in the control group was 332%. The values of the specific growth rate of T. suecica were calculated for different periods of cultivation. After 14 days of the experiment, the biochemical composition of the microalgae biomass was evaluated, which showed stimulation with salicylic acid at a con-centration of 3.75 • 10–5 M carbohydrate accumulation. The high concentration of phytohormone suppressed the accumulation of protein, lipids and chlorophyll in the culture and stimulated the accumulation of carbohydrates. It has been suggested that a possible mechanism for the multidirectional action of salicylic acid is its effect on synthesis and catabolism through inhibition of the synthesis and metabolism of endogenous plant hormones.