Influence of rs6667202 SNP on Interleukin-10 levels in the gingival fluid of patients with periodontitis grade C

Abstract

Grade C periodontitis in youngers is characterized by a severe form of periodontitis, and IL10 rs6667202 single nucleotide polymorphism (SNP) has been described as an important feature in this disease etiology. Aim: This study aimed to evaluate, in vivo, the functionality of IL10 rs6667202 SNP on IL-10 gingival fluid levels. Methods: Thirty patients with Perio4C were selected, 15 with the IL10 AA genotype (rs6667202) and 15 with AC/CC genotypes. The gingival fluid was collected from two sites with probing depth ≥ 7 mm and bleeding on probing, and two healthy sites. The IL-10 concentration was determined by Luminex/MAGpix platform. Results: In deep pockets, the IL10 AA genotype presented a lower concentration of IL-10 when compared with AC or CC genotypes (p<0.05). In shallow pockets, no difference between groups was seen (p>0.05). Conclusion: IL10 rs6667202 SNP decreases the production of IL-10 in crevicular fluid, potentially affecting this disease progression.

IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study

“…Integrating different factors that participate in the etiopathogenesis of Perio4C, Casarin et al, 2010 showed a lower level of IL‐10 in the GCF of individuals with Perio4C while detected a higher concentration of Aa and reduced concentration of immunoglobulin G (IgG) against this pathogen 43 . More recently, a preliminary in vivo study has shown that rs6667202 SNP leads to a decrease in IL‐10 production only in deep pockets of Perio4C patients, where a mature and dysbiotic biofilm is instigating the host response 19 . Teles et al, 2010 correlated bacterial species of the red complex with a higher expression of biomarkers (IL‐1β, IL‐8, and MMP‐8) in GCF, while species of the yellow and purple complex were associated only with MMP‐8.…”

“…12 patients, where a mature and dysbiotic biofilm is instigating the host response. 19 Teles et al, 2010 correlated bacterial species of the red complex with a higher expression of biomarkers (IL-1β, IL-8, and MMP-8) in GCF, while species of the yellow and purple complex were associated only with MMP-8. In addition, even the healthy sites of patients with periodontitis had higher levels of IL-1β and IL-8 and periodontal pathogens than the PH group, showing distinct associations between the host's immune response and microbial complexes.…”

“…They determined the gene expression of IL10, observing a reduction in its mRNA levels on affected patients. 18 Regarding the rs6667202, a recent in vivo preliminary study associated this SNP with lower IL-10 production in the crevicular fluid of Perio4C patients, 19 indicating its potential functional role. Once rs6667202 is located in an enhancer DNA sequence that acts on the IL10 promoter region, regulating its transcription, we can assume that the presence of a variation in this DNA sequence can alter IL-10 production, possibly decreasing it.…”

Perfil taxonômico e funcional do biofilme subgengival de pacientes com periodontite agressiva generalizada em terapia de manutenção

“…New evidence suggests a potential role of host response in establishing dysbiosis and inflammation as a determinant factor in periodontitis pathogenesis (Van Dyke, Bartold, & Reynolds, 2020). In GAgP subjects, a vast number of studies suggested that genetic aspects could determine cytokine profile (Schaefer, 2018;Stolf et al, 2021;Taiete et al, 2019), what can potentially impact the subgingival environment and drives colonization. This comprehension of those interfering with the microbial community and treatment response is of great interest and should be further considered.…”

Funcionalidade do "IL10" SNP rs6667202 em fibroblastos gengivais de pacientes com periodontite grau C

“…Regarding the rs6667202 SNP, a recent in vivo preliminary study indicated its potential functional role, with lower interleukin 10 (IL-10) production in the crevicular fluid (16). Once IL-10 presents regulatory and anti-inflammatory properties, reducing the expression of proinflammatory cytokines, such as interleukin 1β (IL-1β) and tumoral necrosis factor α (TNF-α) (17,18), the higher frequency of rs6667202 SNP in Perio4C population can be explained.…”