Influence of Root Canal Disinfectants on Growth Factor Release from Dentin

Galler, Kerstin M.; Buchalla, Wolfgang; Hiller, Karl‐Anton; Federlin, Marianne; Eidt, Andreas; Schiefersteiner, Mona; Schmalz, Gottfried

doi:10.1016/j.joen.2014.11.021

Cited by 186 publications

(206 citation statements)

References 39 publications

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“…In order to produce new dentin, stem/progenitor cells would have to migrate toward the treated dentine walls, proliferate and differentiate into odontoblasts. 4 In the present study, we used tooth slices with and without the predentin layer, and chemical agents typically employed as root canal irrigants (NaOCl and EDTA), to test if the Table 1. Estimated TGF-b1 concentration (pg/mL) in the medium conditioned by tooth slices either containing or not containing the predentin layer (PD) and treated with PBS, 10% EDTA or 2.5% NaOCl.…”

Section: Discussionmentioning

confidence: 99%

“…The choice of EDTA and NaOCl was based on their widespread use in the solubilization of the dentin matrix and mineral components from the smear layer. 4,8 In contrast, PBS was chosen due to its inability to properly dissolve the mineral or organic components of dentin. 4,8 Considering that NaOCl denatures proteins found in the dentin matrix, 17 we employed this solution for five days to remove the organic content from dentin in an attempt to eliminate the effect of dentin matrix molecules on SHED.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have focused on the regenerative potential of different cytokines and growth factors designed to improve cellular chemotaxis and cell homing into the emptied dental pulp space in vivo. 1,2,3,4,5,6 The dentin matrix is a reservoir of soluble cytokines and growth factors secreted by odontoblasts and pulp fibroblasts, which could be released during tissue demineralization. 1,2,3,4 The dissolution of these cytokines and growth factors can occur pathologically during dental caries and following restorative procedures.…”

Section: Introductionmentioning

confidence: 99%

“…1,2,3,4,5,6 The dentin matrix is a reservoir of soluble cytokines and growth factors secreted by odontoblasts and pulp fibroblasts, which could be released during tissue demineralization. 1,2,3,4 The dissolution of these cytokines and growth factors can occur pathologically during dental caries and following restorative procedures. 2,3 Notably, during severe pulp disease or trauma, primary odontoblasts may not survive, and formation of a Declaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.…”

Section: Introductionmentioning

confidence: 99%

“…6,7,8,9,10,11,12 From a clinical perspective, the use of appropriate irrigation solutions could release the TGF-b1 stored in the dentin, thus contributing to successful regenerative endodontic treatment by directing cellular fate. 4 Two irrigants currently used during cleaning and shaping of the root canal are ethylenediaminetetraacetic acid (EDTA) 13 and sodium hypochlorite (NaOCl). 14, 15,16,17 EDTA is a final irrigant that causes dentin demineralization and provides excellent cleaning of the canal walls.…”

Section: Introductionmentioning

confidence: 99%

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Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

et al. 2016

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Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2.5% NaOCl for 5 days or kept in PBS. Tooth slice conditioned media were prepared and used for TGF-b1 ELISA and migration assays. Culture medium with different concentrations of recombinant human TGF-b1 (0.5, 1.0, 5.0 or 10.0 ng/mL) was also tested by migration assay. The data were evaluated by ANOVA and Tukey's test. Optical density values corresponding to media conditioned by tooth slices either containing or not containing the predentin layer and treated with 10% EDTA were statistically greater than the other groups and close to 1 ng/mL. Increased rates of migration toward media conditioned by tooth slices containing the predentin layer and treated with PBS, 10% EDTA or 2.5% NaOCl were observed. Recombinant human TGF-b1 also stimulated migration of SHED, irrespective of the concentration used. EDTA may be considered an effective extractant of TGF-b1 from the dentin matrix. However, it does not impact SHED migration, suggesting that other components may account for the cell migration.

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Section: Discussionmentioning

confidence: 99%