Influence of river discharge on abundance and dissemination of heterotrophic, indicator and pathogenic bacteria along the east coast of India

Prasad, Vandana; Srinivas, T. N. R.; Sarma, V. V. S. S.

doi:10.1016/j.marpolbul.2015.04.032

Cited by 25 publications

(13 citation statements)

References 32 publications

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“…in spring and summer, respectively. This could be due to the stabilizing effect of suspended particulate matter on bacteria (Gin and Goh, 2013 ; Perkins et al, 2014 ; Prasad et al, 2015 ; Hassard et al, 2016 ), and the enhanced attachment of gammaproteobacteria to particles (Liu et al, 2016 ). Particulate association may mitigate the harmful effects of UV, which could exert a strong effect on bacterial viability in shallow coastal and estuarine regions (Kay et al, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Physicochemical Factors Influence the Abundance and Culturability of Human Enteric Pathogens and Fecal Indicator Organisms in Estuarine Water and Sediment

et al. 2017

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To assess fecal pollution in coastal waters, current monitoring is reliant on culture-based enumeration of bacterial indicators, which does not account for the presence of viable but non-culturable or sediment-associated micro-organisms, preventing effective quantitative microbial risk assessment (QMRA). Seasonal variability in viable but non-culturable or sediment-associated bacteria challenge the use of fecal indicator organisms (FIOs) for water monitoring. We evaluated seasonal changes in FIOs and human enteric pathogen abundance in water and sediments from the Ribble and Conwy estuaries in the UK. Sediments possessed greater bacterial abundance than the overlying water column, however, key pathogenic species (Shigella spp., Campylobacter jejuni, Salmonella spp., hepatitis A virus, hepatitis E virus and norovirus GI and GII) were not detected in sediments. Salmonella was detected in low levels in the Conwy water in spring/summer and norovirus GII was detected in the Ribble water in winter. The abundance of E. coli and Enterococcus spp. quantified by culture-based methods, rarely matched the abundance of these species when measured by qPCR. The discrepancy between these methods was greatest in winter at both estuaries, due to low CFU's, coupled with higher gene copies (GC). Temperature accounted for 60% the variability in bacterial abundance in water in autumn, whilst in winter salinity explained 15% of the variance. Relationships between bacterial indicators/pathogens and physicochemical variables were inconsistent in sediments, no single indicator adequately described occurrence of all bacterial indicators/pathogens. However, important variables included grain size, porosity, clay content and concentrations of Zn, K, and Al. Sediments with greater organic matter content and lower porosity harbored a greater proportion of non-culturable bacteria (including dead cells and extracellular DNA) in winter. Here, we show the link between physicochemical variables and season which govern culturability of human enteric pathogens and FIOs. Therefore, knowledge of these factors is critical for accurate microbial risk assessment. Future water quality management strategies could be improved through monitoring sediment-associated bacteria and non-culturable bacteria. This could facilitate source apportionment of human enteric pathogens and FIOs and direct remedial action to improve water quality.

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Section: Discussionmentioning

confidence: 99%

Physicochemical Factors Influence the Abundance and Culturability of Human Enteric Pathogens and Fecal Indicator Organisms in Estuarine Water and Sediment

et al. 2017

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“…Groundwater is an important land-based source of nutrients entering coastal ecosystems (Hernández-Terrones et al, 2010), and human activities may influence their concentrations (Hernández-Terrones et al, 2015). In previous studies it has been shown that nutrients enhance CB growth and reproduction, particularly inorganic nitrogen (Prasad et al, 2015). This could account for the correlation of nutrients in Jiaozhou Bay with the abundance of CB.…”

Section: Dip No −mentioning

confidence: 99%

“…Previous studies have reported that pollutants and bacterial cells can be carried to coastal bays through estuarine systems (Ridgway and Shimmield, 2002;Prasad et al, 2015), and rainfall can carry pollutants into estuarine areas in surface runoff and groundwater. Consequently, we infer that the abundance of CB may have been influenced by land-sourced pollutants carried into Jiaozhou Bay by rainfall.…”

Section: Dip No −mentioning

confidence: 99%

Long-Term Temporal and Spatial Distribution of Coliform Bacteria in Jiaozhou Bay Associated With Human Activities and Environmental Governance

et al. 2021

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Coliform bacteria (CB) can be used as an indicator of seawater quality. Long-term monitoring of seawater quality based on CB abundance is lacking in Jiaozhou Bay. In this study, CB abundance in surface seawater of 12 different stations in Jiaozhou Bay was investigated by culturing method. The results showed that: (1) the abundance of CB showed a decreasing tendency during the investigation. During 2004–2007, 2008–2013, and 2014–2017, the average CB abundance decreased significantly, forming a “three stages phenomenon”; (2) the average CB abundance in the first half of the year was lower than that in the second half; (3) the CB abundance in Jiaozhou Bay was spatially heterogeneous. The maximum average CB abundance was observed in the estuary area, and followed by the bay mouth area, the outer bay area, and the inner bay area. The highest abundance may be associated with sewage discharge related to human activities; (4) the abundance of CB was most positively correlated with the concentration of ammonium salt and nitrate, while most negatively correlated with salinity; (5) the years 2007 (2008) and 2013 (2014) were time points of the “three stages phenomenon.” These time points coincide with environmental governance actions, indicating that the actions have played a prominent role in improving seawater quality. Long-term survey of CB can not only serve as an indicator of seawater quality, but also provide a basis for the development of environmental governance strategies and pollution control.

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“…Knowledge of the time required for water column bacteria to incorporate into the biofilm of macroalgae would have important implications in decisions based on when to harvest following anthropogenic or environmental events that may increase bacterial load (Lu et al 2008;Liu and Pang 2010). The winter-spring sampling season coincided with spring snowmelt and rain events that increased freshwater discharge from local rivers and terrestrial runoff and are often linked to increased fecal bacteria levels in coastal waters (Online Resource B; Prasad et al 2015;Tilburg et al 2015;Jokinen et al 2009).…”

Section: Kelp-seawater Relationshipmentioning

confidence: 99%

Assessment of bacterial pathogens on edible macroalgae in coastal waters

et al. 2019

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In the Northeast USA, the aquaculture of macroalgae is a rapidly growing industry. Within this region, there are no established regulations for farm siting or methods of pathogen detection on macroalgae cultivated or harvested for human consumption. Bacterial pathogens from natural and anthropogenic sources may persist in coastal waters and can potentially contaminate macroalgae. During the winter growing season, sugar kelp Saccharina latissima and adjacent water were sampled from three sites of kelp aquaculture located in adjacent bays of ME, USA. Membrane filtration onto selective media detected Escherichia coli, Vibrio parahaemolyticus, and Vibrio alginolyticus in kelp and water samples at all sites, however plate counts were very low. The foodborne pathogens Salmonella enterica ser. Typhimurium, V. parahaemolyticus, and enterohemorrhagic E. coli O157:H7 were detected on enriched kelp samples from 83%, 78%, and 56% of sampling events, respectively, using molecular methods. Even with low bacterial levels, this frequency of detection confirms the risk of foodborne pathogens present on kelp and recommends the development of best management practices to control microbial growth during kelp harvest and processing. Bacterial plate counts from kelp samples often varied from those of water, indicating the importance of sampling the kelp directly, and that the association between bacterial pathogens on kelp and in the surrounding water should be further investigated. This study provides the first food safety assessment of sea vegetables in this region with the goal of providing data to enable the expansion of its industry.

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Influence of river discharge on abundance and dissemination of heterotrophic, indicator and pathogenic bacteria along the east coast of India

Cited by 25 publications

References 32 publications

Physicochemical Factors Influence the Abundance and Culturability of Human Enteric Pathogens and Fecal Indicator Organisms in Estuarine Water and Sediment

Physicochemical Factors Influence the Abundance and Culturability of Human Enteric Pathogens and Fecal Indicator Organisms in Estuarine Water and Sediment

Long-Term Temporal and Spatial Distribution of Coliform Bacteria in Jiaozhou Bay Associated With Human Activities and Environmental Governance

Assessment of bacterial pathogens on edible macroalgae in coastal waters

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