Influence of RGD grafting on biocompatibility of oxidized cellulose scaffold

Mahmoodi, Mozaffar; Hossainalipour, S. M.; Naimi-Jamal, Mohammad Reza; Samani, Saeed; Samadikuchaksaraei, Alí; Rezaie, Hamid Reza

doi:10.3109/21691401.2012.759123

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…One hundred microliters of the supernatant obtained from each group was transported into 96‐well cell culture plates and the absorbance of the formazon product was measured at 570 nm using an Elisa reader (Elx 808 biotek, USA). Moreover, specific growth rate [(µ m) h −1 ] was calculated using [Ln A / A 0 = μ ( t − t 0 )] formula applying the absorbance data . In the formula, A 0 is the initial absorbance at t 0 (h) and A is the absorbance at t (h).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

Rashtbar

Hadjati

et al. 2017

J Biomed Mater Res

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Extracellular matrix-based scaffolds derived from mammalian tissues have been used in tissue engineering applications. Among all the tissues, decellularized small intestine submucosal layer (SIS) has been recently investigated for its exceptional characteristics and biocompatibilities. These investigations have been mainly focused on the decellularized porcine SIS; however, there has not been any report on ovine SIS (OSIS) layer. In this study, OSIS was decellularized and its physical, chemical, and morphological properties were evaluated. Decellularization was carried out using chemical reagents and various physical conditions. The effects of different conditions were evaluated on histological and biomechanical properties, quality of residual DNA, GAPDH gene expression, and biocompatibility. Results revealed satisfactory decellularization of OSIS which could be due to its thin thickness. Mechanical properties, structural form, and glycosaminoglycan contents were preserved in all the decellularized groups. In SDS-treated groups, further cells and DNA residues were removed compared to the groups treated with Triton X-100 only. No toxicity was observed in all treatments, and viability, expansion, and cell proliferation were supported. In conclusion, our results suggest that OSIS decellularized scaffold could be considered as an appropriate biological scaffold for tissue engineering applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 933-944, 2018.

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Section: Methodsmentioning

confidence: 99%

“…Moreover, specific growth rate [(m m) h 21 ] was calculated using [Ln A/A 0 5 l(t 2 t 0 )] formula applying the absorbance data. 18 In the formula, A 0 is the initial absorbance at t 0 (h) and A is the absorbance at t (h).…”

Section: Viability and Proliferation Assaymentioning

confidence: 99%

Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

Rashtbar

Hadjati

et al. 2017

J Biomed Mater Res

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“…Supernatant of each group transport was transported to 96‐well cell cultures at a volume of 100 µL and the absorbance of the formazan product was measured at 570 nm using an Elisa reader (Elx808‐biotek, USA). Furthermore, assuming that the cell growth was exponential, special growth rate (μ, h −1 ) was calculated by fitting [Ln A / A 0 = μ( t − t 0 )] formula to the absorbance data, where A 0 is initial absorbance at t 0 (h) and A is absorbance at t (h)] …”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, assuming that the cell growth was exponential, special growth rate (l, h 21 ) was calculated by fitting [Ln A/A0 5 l(t 2 t 0 )] formula to the absorbance data, where A0 is initial absorbance at t0 (h) and A is absorbance at t(h)]. 25 Scanning electron microscopy (SEM) SEM investigation was used to study the attachment of the cultured cells on the scaffold. BM-MSCs were seeded at a density of 1 3 10 4 per each scaffold.…”

Section: Viability and Proliferation Assaymentioning

confidence: 99%

Critical‐sized full‐thickness skin defect regeneration using ovine small intestinal submucosa with or without mesenchymal stem cells in rat model

Rashtbar

Hadjati

et al. 2017

J Biomed Mater Res

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Decellularized extracellular matrices (ECM) based materials are routinely used for a variety of clinical applications. Hereof, in vivo application of decellularized ovine small intestinal submucosal (DOSIS) layer as, a scaffold is yet to be investigated. In this study, the effectiveness of the DOSIS scaffold, with or without rat bone marrow mesenchymal stem cells (BM-MSCs), in full-thickness wound healing of critical-sized defect was experimentally studied in a rat model. The experimental groups included; group I (control), group II (DOSIS), and group III (BM-MSCs-seeded DOSIS). Wound healing of all groups was examined and compared clinically and histopathologically on days 7, 14, and 21 postoperation. Our results represented BM-MSCs-seeded DOSIS accelerated wound contraction and healing compared to both the DOSIS alone and control groups. Epithelization was close to completion 21 days postoperation in DOSIS alone. In OSIS with BM-MSCs group, epithelization was faster and had fully taken place at the subsequent time points. DOSIS layer, as cell-free form with low substantially DNA content, accelerated healing of rat skin wound defects that was created at critical-size and full-thickness. In conclusion, decellularized OSIS alone and in combination with BM-MSCs has the potential to be used as a wound graft material in skin regenerative medicine. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2177-2190, 2018.

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“…In order to increase the healing properties, the bioactive compounds have been widely studied and used in the structure of wound dressings. 4 Different bioactive compounds have different effect in cells behavior and tissue healing. A frequently used source of bioactive compounds, which promote wound healing, is extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of a hydrocolloid wound dressing functionalized with human placental derived extracellular matrix for management of skin wounds: An animal study

Jafari,

Gholipourmalekabadi,

Alizadeh

et al. 2023

Artificial Organs

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BackgroundFunctionalization of wound dressing is one of the main approaches for promoting wound healing in skin wound management. In this study, our aim is to fabricate a bio‐functionalized hydrocolloid wound dressing.MethodsThe extracellular matrix (ECM) was extracted from human placental tissue. A hydrocolloid film was fabricated using Na‐CMC, pectin, gelatin, styrene‐isoprene‐styrene adhesive, glycerol, and 0.5%–2.5% powdered ECM. A polyurethane film and a release liner were used in the hydrocolloid/ECM films. The mechanical, adhesion, swelling rate, and integrity of the films were investigated. Cell proliferation, adhesion, and migration assays, as well as, SEM and FTIR spectroscopy were also conducted. Macroscopic and microscopic evaluations of wound healing process and formation of blood vessels were conducted in mouse animal models.ResultsWe successfully fabricated a three‐layered ECM‐functionalized hydrocolloid dressing with a water vapor transmission rate of 371 g/m2/day and an adhesion peel strength of 176 KPa. Cellular adhesion, proliferation and migration were promoted by ECM. In the animal tests, ECM‐functionalized hydrocolloids significantly improved wound closure and re‐epithelialization at days 14 and 21. Also, ECM‐functionalized hydrocolloids promoted the formation of hair follicles.ConclusionsOur findings suggest that ECM could enhance the wound healing properties of hydrocolloid wound dressings. This wound dressing could be considered for application in hard‐to‐heal acute wounds.

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Influence of RGD grafting on biocompatibility of oxidized cellulose scaffold

Cited by 7 publications

References 17 publications

Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

Critical‐sized full‐thickness skin defect regeneration using ovine small intestinal submucosa with or without mesenchymal stem cells in rat model

Fabrication and characterization of a hydrocolloid wound dressing functionalized with human placental derived extracellular matrix for management of skin wounds: An animal study

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