Influence of Redox Potential on Product Distribution in Clostridium thermosuccinogenes

Sridhar, Jayanth; Eiteman, Mark A.

doi:10.1385/abab:82:2:91

Cited by 25 publications

(16 citation statements)

References 17 publications

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“…From the initial assemblies, it was clear that DSM 5806, DSM 5807 T , and DSM 5808 were quite similar, while DSM 5809 was more different from the others (see below). In an attempt to reduce the number of contigs, DSM 5807 T was selected for PacBio sequencing as the type strain and representative of DSM 5806 and DSM 5808, together with DSM 5809, which appears to produce the most succinic acid (5,6). The data from both Illumina and PacBio were combined in a hybrid assembly, which resulted in a closed genome for DSM 5807 T and an assembly of 8 scaffolds for DSM 5809.…”

Section: Resultsmentioning

confidence: 99%

“…Although it belongs to a group of (hemi)cellulolytic organisms and is a close relative of the industrially relevant thermophilic Clostridium thermocellum and mesophilic Clostridium cellulolyticum, it is incapable of degrading (hemi)cellulose. A physiological characterization of the only four strains of the species that have been described (DSM 5806, DSM 5807 T , DSM 5808, and DSM 5809) includes an evaluation of the effect of pH and redox potential on the distribution of its fermentation products, namely, SA, acetic acid, formic acid, lactic acid, ethanol, and hydrogen (6)(7)(8). Furthermore, enzyme assays have been used to shed light on the fermentation pathways toward these products.…”

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confidence: 99%

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Investigating the Central Metabolism of Clostridium thermosuccinogenes

Koendjbiharie

Wiersma

Kranenburg

2018

Appl Environ Microbiol

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is a thermophilic anaerobic bacterium able to convert various carbohydrates to succinate and acetate as main fermentation products. Genomes of the four publicly available strains have been sequenced, and the genome of the type strain has been closed. The annotated genomes were used to reconstruct the central metabolism, and enzyme assays were used to validate annotations and to determine cofactor specificity. The genes were identified for the pathways to all fermentation products, as well as for the Embden-Meyerhof-Parnas pathway and the pentose phosphate pathway. Notably, a candidate transaldolase was lacking, and transcriptomics during growth on glucose versus that on xylose did not provide any leads to potential transaldolase genes or alternative pathways connecting the C with the C/C metabolism. Enzyme assays showed xylulokinase to prefer GTP over ATP, which could be of importance for engineering xylose utilization in related thermophilic species of industrial relevance. Furthermore, the gene responsible for malate dehydrogenase was identified via heterologous expression in and subsequent assays with the cell extract, which has proven to be a simple and powerful method for the basal characterization of thermophilic enzymes. Running industrial fermentation processes at elevated temperatures has several advantages, including reduced cooling requirements, increased reaction rates and solubilities, and a possibility to perform simultaneous saccharification and fermentation of a pretreated biomass. Most studies with thermophiles so far have focused on bioethanol production. seems an attractive production organism for organic acids, succinic acid in particular, from lignocellulosic biomass-derived sugars. This study provides valuable insights into its central metabolism and GTP and PP cofactor utilization.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Investigating the Central Metabolism of Clostridium thermosuccinogenes

Koendjbiharie

Wiersma

Kranenburg

2018

Appl Environ Microbiol

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“…One of the major conclusions drawn from those studies was that each species, or even each strain, has a preferable redox potential range, within which cell growth is possible. In addition, extracellular ORP was shown to affect the synthesis or stability of certain enzymes, which, in turn, could change metabolic fluxes and/or ATP yield [11][12][13][14]. Furthermore, ORP has been successfully used to optimize and scale-up some fermentation processes, such as citric acid and xylitol production [3,15].…”

Section: Introductionmentioning

confidence: 99%

Effect of redox potential regulation on succinic acid production by Actinobacillus succinogenes

Jiang

Chen

et al. 2010

Bioprocess Biosyst Eng

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The effects of redox potential used as a control parameter on the process of succinic acid production in batch cultures of Actinobacillus succinogenes NJ113 have been investigated. In batch fermentation, cell growth and metabolite distribution were changed with redox potential levels in the range of -100 to -450 mV. From the results, the ORP level of -350 mV was preferable, which resulted in high succinic acid yield (1.28 mol mol(-1)), high succinic acid productivity (1.18 g L(-1) h(-1)) and high mole ratio of succinic acid to acetic acid (2.02). The mechanism of redox potential regulation was discussed by metabolic flux analysis and the ratio of NADH/NAD+. We expected that redox potential can be used as a valuable parameter to monitor and control much more anaerobic fermentation production.

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“…Biomass derived from JA can also be converted to butanol and acetone by Clostridium sp. strains (Montoya et al 2001), succinic acid and acetic acid by Clostridium thermosuccinogenes (Sridhar and Eiteman 1999), and sorbitol by S. cerevisiae ATCC 36859 (Duvnjak et al 1991).…”

Section: Biochemical Productsmentioning

confidence: 99%

Biorefinery products from the inulin-containing crop Jerusalem artichoke

Li²,

Wang

et al. 2012

Biotechnol Lett

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The polysaccharides in Jerusalem artichoke (JA) carry a substantial amount of energy that can be partly accessed through bioconversion into storable fuels. We review the potential for converting inulin into a variety of high value-added biorefinery products, including biofuels and biochemicals, and consider the feasibility of regarding JA as a model species of an inulin-rich crop. We discuss feedstock pretreatment, microorganisms used during fermentation, biorefinery products derived from JA, and how to enhance the economic competitiveness of JA as an energy crop.

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Influence of Redox Potential on Product Distribution in Clostridium thermosuccinogenes

Cited by 25 publications

References 17 publications

Investigating the Central Metabolism of Clostridium thermosuccinogenes

Investigating the Central Metabolism of Clostridium thermosuccinogenes

Effect of redox potential regulation on succinic acid production by Actinobacillus succinogenes

Biorefinery products from the inulin-containing crop Jerusalem artichoke

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