Influence of processing and storage of integral grape juice (Vitis labrusca L.) on its physical and chemical characteristics, cytotoxicity, and mutagenicity in vitro

Abstract

ABSTRACT. Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) in vitro. Vitis labrusca L. (variety Concord) were produced organically and by a conventional system, and their juice was extracted by a hot extraction process. The organic grapes were subjected to ultraviolet-type C radiation (UV-C). Experiments were performed after production and after 6 months in storage. Physicochemical analyses revealed that UV-C irradiation of 2 E. Düsman et al. (2): gmr16029670 organic grapes, the juice production process, and storage resulted in nutraceutical alterations. However, none of the juice concentrations were cytotoxic to HTC cells by the cytokinesis-blocked proliferation index results or were mutagenic, because the formation of micronucleated cells was not induced. In general, juice induced cell proliferation, possibly due to the presence of vitamins and sugar content (total soluble solid). The data increased the understanding of food technology and confirmed the quality and safety consumption of these juices. Genetics and Molecular Research 16

Production and Evaluation of Low-Calorie Pomegranate Juice with Sucralose

“…Reduction in acidity could be attributed to chemical interaction between organic constituents of juice induced by temperature and action of enzymes during storage (Sindhu & Khatkar, 2018). Similar results were found by (Dusman et al, 2017) who reported that the acidity decreased in organic grape juice during storage for 6 months, could be due to the copolymerization of organic acids with other compounds present in their products.…”

Research Article Cytotoxic effects of <i>Vitis</i> <i>labrusca</i> (fox grape) whole juices on human tumor and non-tumor cells, <i>in</i> <i>vitro</i>

“…The cytotoxic effects of the conventional grape juices were not found by Düsman et al (2014Düsman et al ( , 2017 who evaluated the same whole juices of the present study (Düsman et al, 2014) and the juices produces from the harvest of 2011 (Düsman et al, 2017) in Rattus norvegicus hepatoma cells (HTC). However, Düsman et al (2014) noted that the conventional whole grape juices that were treated or not with UV-C, induced the smallest cytokinesis-block proliferation index, as well the highest average number of micronuclei.…”

The effect of grapes/grape products on glycemic response: A systematic review and meta‐analysis of randomized controlled trials

“…It should be noted that factors affecting glycemic control often manifest differently among racial and ethnic groups and can have individual variations across a person lifespan (Harris, Eastman, Cowie, Flegal, & Eberhardt, 1999). Furthermore, the discrepancy in the context of grapes variety and cultivation conditions (Toaldo, Cruz, da Silva, & Bordignon‐Luiz, 2016), production process, and storage which greatly influence the biological activity of grape extracts (Dusman, Almeida, Pinto, Lucchetta, & Vicentini, 2017), could affect the results. Also, the diversely used methods and kits for measuring variables may contribute to the variation in clinical response (Herman, 2009).…”