Research Article Cytotoxic effects of &lt;i&gt;Vitis&lt;/i&gt; &lt;i&gt;labrusca&lt;/i&gt; (fox grape) whole juices on human tumor and non-tumor cells, &lt;i&gt;in&lt;/i&gt; &lt;i&gt;vitro&lt;/i&gt;

Lopes, Nuno Santos; Almeida, Igor Vivian de; Luchetta, L.; Düsman, Elisângela; Vicentini, Verônica Elisa Pimenta

doi:10.4238/gmr18236

Cited by 3 publications

(5 citation statements)

References 36 publications

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“…Nevertheless, it is noteworthy that within 48 hours, UV-C conventional grape juice (20 µL/mL), organic grape juice (20 µL/mL) and UV-C organic grape juice (10 and 20 µL/mL) showed a cytotoxic effect on HepG2/C3A cells. These data corroborate the work of Lopes et al (2019), in which the same juices evaluated in the present study, at concentrations of 10 to 100 µL/mL, presented antitumor activity for HepG2/C3A cells, especially for the highest concentrations, in the longest exposure time and with UV-C postharvest treatment. According to El-Din et al ( 2019), grape seed and peel extracts showed antitumor effect by cell cycle arrest, apoptosis induction and inhibition of cell proliferation.…”

Section: Resultssupporting

confidence: 92%

“…For the cytotoxicity test, the MTT assay was performed as described by Lopes et al (2019). The 96-well cell culture plates were used, wherein each well 10 4 HepG2/C3A or MRC-5 cells were seeded.…”

Section: Mtt Cytotoxicity Testmentioning

confidence: 99%

“…After 24 or 48 hours of incubation, the culture medium was replaced with MTT (0.2 mg/mL) and the absorbance readings were performed in a 550nm microplate reader (Lopes et al, 2019).…”

Section: Mtt Cytotoxicity Testmentioning

confidence: 99%

“…In this sense, grapes (Vitis labrusca L.) and their derivatives stand out, which have numerous nutritional, pharmacological and therapeutic benefits (Apostolou et al, 2013;Kim et al, 2013;Huang et al, 2015;Devi and Singh, 2017;Lopes et al, 2019), mainly due to the presence of phenolic compounds and their antioxidant activity (Fernandes et al, 2013;Maestre et al, 2013;Pinto et al, 2022).…”

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confidence: 99%

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Cytotoxicity of iodine-131 radiopharmaceutical in tumor and non-tumor human cells and radioprotection by integral juices of Vitis labrusca L.

et al. 2022

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Iodine-131 (I-131) radioisotope it causes the formation of free radicals, which lead to the formation of cell lesions and the reduction of cell viability. Thus, the use of radioprotectors, especially those from natural sources, which reduce the effects of radiation to healthy tissues, while maintaining the sensitivity of tumor cells, stands out. The objective of the present study was to evaluate the cytoprotective/radioprotective effects of whole grape juices manufactured from the conventional or organic production systems, whether or not exposed to ultraviolet (UV-C) light irradiation. The results showed that I-131 presented a cytotoxic effect on human hepatocellular cells (HepG2/C3A) at concentrations above 1.85 MBq/mL, after 24 and 48 hours of treatment, though all concentrations (0.0037 to 7.40 MBq/mL) were cytotoxic to non-tumor human lung fibroblast (MCR-5) cells, after 48 hours. However, grape juices (10 and 20 µL/mL) did not interfere with the cytotoxic effect of the therapeutic dose of I-131 on tumor cells within 48 hours of treatment, while protecting the non-tumor cells, probably due to its high antioxidant activity. In accordance with their nutraceutical potential, antioxidant and radioprotective activity, these data stimulate in vivo studies on the use of natural products as radioprotectants, such as grape juice, in order to confirm the positive beneficial potential in living organisms.

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Section: Resultssupporting

confidence: 92%

Section: Mtt Cytotoxicity Testmentioning

confidence: 99%

“…After 24 or 48 hours of incubation, the culture medium was replaced with MTT (0.2 mg/mL) and the absorbance readings were performed in a 550nm microplate reader (Lopes et al, 2019).…”

Section: Mtt Cytotoxicity Testmentioning

confidence: 99%

mentioning

confidence: 99%

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Cytotoxicity of iodine-131 radiopharmaceutical in tumor and non-tumor human cells and radioprotection by integral juices of Vitis labrusca L.

et al. 2022

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“…Considerando-se a capacidade de microconstituintes alimentares inibirem a viabilidade de células tumorais, Heng et al (2017) utilizaram o ensaio de MTT para monitorar o efeito da incubação de 16 diferentes extratos de frutas em células CRFK (células renais) e observaram que os extratos de mirtilo, uva, ameixa e maçã verde foram os que apresentaram maior efetividade na inibição da viabilidade celular. Em relação a extrato de frutas tropicais, alguns estudos apresentam efeito anticâncer (LOPES et al, 2019;FIDELIS et al, 2020;BOEING et al, 2020).…”

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