Influence of Preservative Concentration, pH Value and Fat Content in Raw Milk at Detection Limit of Microbial Inhibitor Tests (Delvotest® Accelerator) for Amoxicillin and Oxytetracycline

Mirecki, Slavko; Nikolic, N.

doi:10.1007/s12161-016-0476-8

Cited by 6 publications

(2 citation statements)

References 10 publications

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“…Second, bivalent metal ions in milk such as Ca 2+ can chelate tetracyclines, resulting in weaker bacteriostatic ability of tetracyclines (Kuang et al, 2009). Third, the bacteriostatic activity of tetracyclines is stronger in a slightly acidic environment than in an alkaline condition (Mirecki and Nikolić, 2016). Chloramphenicol is widely used as a sensitizer of tetracyclines in microbiological inhibition methods.…”

Section: Detection Capabilitymentioning

confidence: 99%

A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk

Zhu

Liu

et al. 2019

Journal of Dairy Science

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In this study, we developed a microbiological inhibition method for the rapid screening of antibiotics in milk with Geobacillus stearothermophilus ATCC12980 as an indicator bacterium and an easy sample pretreatment. We observed that the limits of detection of the kit for 34 common antibiotic residues in milk, including β-lactams (13), aminoglycosides (6), tetracyclines (4), sulfonamides (6), macrolides (4), lincosamides (1), were lower than or close to the maximum residue limits formulated by the European Union and China. Moreover, the false-positive rate was 1% and the falsenegative rates were less than 5%. The ruggedness of the method (the reproducibility of detection capability of different batches of medium) met requirements at determined levels and residual limits. The shelf life of the kit was more than 6 mo at 4°C. Additionally, we observed good correlations between the kit results and ultra-high-performance liquid chromatography-tandem mass spectrometry results for incurred milk (samples taken from animals treated with antibiotics according to the pre-slaughter medication data), which indicated that the kit was reliable for screening antibiotics in incurred samples. In conclusion, the kit has a broad application potential with high sensitivity, specificity, and reproducibility, stability, and reliability, combined with simple operation, low cost, and high-throughput capacity.

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Section: Detection Capabilitymentioning

confidence: 99%

A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk

Zhu

Liu

et al. 2019

Journal of Dairy Science

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show abstract

“…A microbiological assay is time-consuming and has poor specificity for the target due to the interference of detection duration and the presence of active antibacterial substances (enzymes, antibodies, etc.) in milk [20,21]. Immunoassays are characterized by specificity, high throughput, and sensitivity, as well as low cost and easy operation.…”

Section: Introductionmentioning

confidence: 99%

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Wang

Yang

et al. 2021

Foods

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Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.

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Effects of Lactiplantibacillus plantarum WLPL01 fermentation on antioxidant activities, bioactive compounds, and flavor profile of Artemisia argyi

Zhan¹,

Xu²,

Zhao³

et al. 2022

Food Bioscience

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Influence of Preservative Concentration, pH Value and Fat Content in Raw Milk at Detection Limit of Microbial Inhibitor Tests (Delvotest® Accelerator) for Amoxicillin and Oxytetracycline

Cited by 6 publications

References 10 publications

A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk

A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Effects of Lactiplantibacillus plantarum WLPL01 fermentation on antioxidant activities, bioactive compounds, and flavor profile of Artemisia argyi

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