A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk

Abstract

In this study, we developed a microbiological inhibition method for the rapid screening of antibiotics in milk with Geobacillus stearothermophilus ATCC12980 as an indicator bacterium and an easy sample pretreatment. We observed that the limits of detection of the kit for 34 common antibiotic residues in milk, including β-lactams (13), aminoglycosides (6), tetracyclines (4), sulfonamides (6), macrolides (4), lincosamides (1), were lower than or close to the maximum residue limits formulated by the European Union and China. Moreover, the false-positive rate was 1% and the falsenegative rates were less than 5%. The ruggedness of the method (the reproducibility of detection capability of different batches of medium) met requirements at determined levels and residual limits. The shelf life of the kit was more than 6 mo at 4°C. Additionally, we observed good correlations between the kit results and ultra-high-performance liquid chromatography-tandem mass spectrometry results for incurred milk (samples taken from animals treated with antibiotics according to the pre-slaughter medication data), which indicated that the kit was reliable for screening antibiotics in incurred samples. In conclusion, the kit has a broad application potential with high sensitivity, specificity, and reproducibility, stability, and reliability, combined with simple operation, low cost, and high-throughput capacity.

Assessing Antibiotic Residues in Poultry Eggs from Backyard Production Systems in Chile, First Approach to a Non-Addressed Issue in Farm Animals

“…Therefore, considering the scope of the present study, the selected methodology is suitable to provide information that allows an appropriate overview of the current status of antibiotic residues in poultry from small farms. The screening approach to detect antibiotic residues in food samples has been used for similar purposes by different authors, due to their advantages such as a short analysis time, simplicity and low cost, especially microbial screening methods, due to their high cost-effectiveness [36][37][38][39][40][41]. According to Gaudin et al 2017, eggs are a matrix where screening methods with a wider spectrum of detection have good applicability since few methods have been developed and published for this matrix.…”

Safety Issues Regarding the Detection of Antibiotics Residues, Microbial Indicators and Somatic Cell Counts in Ewes’ and Goats’ Milk Reared in Two Different Farming Systems

“…For such a task the required test should be able to detect the residues in relatively low concentrations and it should be relatively simple, sensitive to a large range of antibiotics and reliable. In other words, a suitable test should be able to detect the residues at concentrations above the MRL (Maximum Residue Level) values as they are stated in the legislation, it should be simple enough to be performed by a relatively unskilled person belonging to the personnel of the farm (or with a minimal training) and it should be performed by a low-cost method (the equipment included) [23,43].…”

Development of Novel Peptide-Modified Silver Nanoparticle-Based Rapid Biosensors for Detecting Aminoglycoside Antibiotics

“…To date, several commercially available rapid test kits have been introduced for detecting various classes of antibiotics, relying on microbial inhibition assays. − However, these assays do have some limitations, including the possibility of false positive results, challenges in reproducibility due to AMR, and higher detection limits compared to the MRLs set by regulatory authorities . Consequently, alternative methods have been explored to improve the detection of AGA residues in environmental samples and food products.…”