Influence of preoperative radiotherapy on DNA ploidy in squamous cell carcinomas of the oesophagus.

Porschen, Rainer; Bevers, Gerd; Remy, Udo; Schauseil, S; Borchard, F

doi:10.1136/gut.34.8.1086

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…although not all oesophageal carcinomas exhibit aneuploidy on DNA analysis (Dorman et al 1992: Porschen et al 1993: Blount et al 1994) and the sensitivity and specificity of the detection of aneuploidy by flow cytometry is only 79% and 60%7 respectively British Joumal of Cancer (1998) 78(7). 950-957…”

Section: Percentage Loh On Each Chromosomal Armmentioning

confidence: 99%

Allelotype analysis of oesophageal adenocarcinoma: loss of heterozygosity occurs at multiple sites

Dolan

Garde²,

Gosney³

et al. 1998

Br J Cancer

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Summary Deletions of tumour-suppressor genes can be detected by loss of heterozygosity (LOH) studies, which were performed on 23 cases of adenocarcinoma of the oesophagus, using 120 microsatellite prmers coverng all non-acrocentric autosomal chromosome arms.The chromosomal arms most frequently demonstrating LOH were 3p (644% of tumours). 5q (45%), 9p (52%), lip (61%) Correspondence to: JK Field studies can lead to the identification of tumour-suppressor genes that are inactix ated in the metaplasia-dxsplasia-carcinoma progression. and may therefore be useful as biomarkers of future carcinogenesis in patients w-ith Barrett's metaplasia and dx splasia undergoing endoscopic surveillance. Previous alleleotype analx ses have detected LOH in more than 40%c of oesophageal adenocarcinomas on chromosome arms lp. 4q. 5q. 9p. 13q. 17p and 18q (Barrett et al. 1996a andHammoud et al. 1996). These allelotype studies AWere undertak-en Awith 43 and 39 microsatellite primers respectixely. We have performed the most comprehensive genomic study of oesophageal adenocarcinoma to date. coxering all of the non-acrocentnrc chromosome arms w-ith 120 microsatellite pnrmers. enabling identification of putatiVe tumour-suppressor gene sites in oesophageal adenocarcinoma. MATERIALS AND METHODS PatientsTwentv-three cases of adenocarcinoma of the oesophagus diagnosed betmeen 1992 and 1996 were studied. Twenty of these patients were male and their mean age xxas 68 y-ears. At present. six of these patients are alix e w ith no signs of recurrent disease. DNA extractionTissue from the tumour and from normal gastric mucosa were obtained from endoscopic biopsies and from surgical resections. snapped frozen in liquid nitroaen and stored at -70 C. Areas of tumour containincg minimal stromal cells wxere microdissected and DNA extracted from the microdissected tissues usinc the Nucleon II extraction kit (Scotlab).950

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Section: Percentage Loh On Each Chromosomal Armmentioning

confidence: 99%

Allelotype analysis of oesophageal adenocarcinoma: loss of heterozygosity occurs at multiple sites

Dolan

Garde²,

Gosney³

et al. 1998

Br J Cancer

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Aneuploidy in upper gastro-intestinal tract cancers—A potential prognostic marker?

Doak

2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Experimental study on radiational characteristics and nursing care of a novel radioisotope 188Re memory alloy esophageal stent

Chen,

Wang,

Huang

et al. 2024

Diseases of the Esophagus

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Summary Radioactive esophageal stent, known for inhibiting tumor growth and delaying restenosis in malignant esophageal tumors, presents challenges due to potent radiation, leading to side effects. This study aims to support the clinical use of 188Re radioactive esophageal stent. The 188Re stent with 128 MBq initial activity was placed in a biomimetic esophageal membrane. Radiation absorption doses were measured by thermoluminescence and calculated using mathematical software. Under simulated positioning, the stent was implanted in the esophagus of an experimental pig, followed by the feeding of Kangfuxin solution and nursing care (KFX-RT). Non-implanted and implanted-only pigs served as normal (CR) and experimental (RT) controls. Blood samples collected on days 7 and 21 were analyzed for inflammatory factors (TGF-β1, TNF-α, IL-6) using enzyme-linked immunosorbent assay. Esophageal tissue cells were assessed for deoxyribonucleic acid index (DI) and subdiploid content through flow cytometry. Absorbed doses at 0.5 mm and 5 mm reference points were 223.91 cGy and 20.55 cGy, respectively, with 92.64% absorbed within a 1 mm thickness. Radiation dose significantly decreased at 6.5 mm, with only 4.72% absorbed at depths ≥6.5 mm. On days 7 and 21, levels of inflammatory factors, DI and subdiploid content were significantly increased in the KFX-RT and RT groups compared to the CR group, while all levels in the KFX-RT group were significantly lower than in the RT group. The 188Re esophageal stent exhibits high radiation absorption in superficial tissues and low absorption in deeper tissues. Kangfuxin solution combined with nursing care alleviates radiation-induced inflammatory damage.

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Influence of preoperative radiotherapy on DNA ploidy in squamous cell carcinomas of the oesophagus.

Cited by 4 publications

References 26 publications

Allelotype analysis of oesophageal adenocarcinoma: loss of heterozygosity occurs at multiple sites

Allelotype analysis of oesophageal adenocarcinoma: loss of heterozygosity occurs at multiple sites

Aneuploidy in upper gastro-intestinal tract cancers—A potential prognostic marker?

Experimental study on radiational characteristics and nursing care of a novel radioisotope 188Re memory alloy esophageal stent

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