Aneuploidy in upper gastro-intestinal tract cancers—A potential prognostic marker?

Genomic Alterations and Allelic Imbalances Are Strong Prognostic Predictors in Osteosarcoma

“…Furthermore, patients with tumors showing above average LOH scores developed recurrent disease more often than did patients with tumors showing low LOH scores. A correlation between chromosomal imbalances and the prognosis of patients has recently been shown not only in osteosarcomas, but also in other tumors, including gastric carcinomas, but the biological basis of these observations is poorly understood (13,17,18). The high LOH scores in osteosarcomas with a poor response to chemotherapy found in our study might represent an imbalanced loss of various genes involved in cell cycle and apoptosis regulation and therefore might reflect a more aggressive phenotype with a potential survival advantage against chemotherapy.…”

“…It has already been shown in other tumors, such as gastrointestinal tract carcinomas, that a high complexity of chromosomal instability is correlated with an unfavorable outcome (13). In this study, we describe the use of Affymetrix single nucleotide polymorphism (SNP) arrays in a genome-wide highresolution approach.…”

A rapid and optimization-free procedure allows the in vivo detection of subtle cell cycle and ploidy alterations in tissues by flow cytometry

“…Ploidy measurements can provide useful information for the characterization of experimental tumor models and, in clinical settings, have been considered of prognostic value. [15][16][17][18] However, necrotic areas, frequently present in tumors, severely affect peak discrimination and impair the accurate determination of subtle ploidy alterations due to substantial amounts of DNA containing cell debris (signal noise). Given the excellent resolution obtained with our method in normal tissue we reasoned that we may be able to detect subtle ploidy alterations in heterogeneous tumors even in the presence of large necrotic parts.…”

The Applicable Use of the HPRT Gene Mutation Assay as a Practical Tool in Mutagenesis and DNA Repair Studies

“…Our group in Swansea University has recently used the AHH-1 cell line to investigate the dose-response relationships of DNA reactive genotoxic agents in the low-dose region of exposure [13,14]. The AHH-1 cell line was very suitable for these experiments, and because it is heterozygous at the TK +/− locus, we were able to validate our HPRT results by carrying out both the HPRT and TK gene mutation assays alongside one another.…”