Influence of polyamines on membrane functions

Schuber, Francis

doi:10.1042/bj2600001

Cited by 420 publications

(231 citation statements)

References 153 publications

(152 reference statements)

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“…The significant decrease in cell viability, possibly due to the leakage of cellular inclusions or cell rupture by downshock, observed in the Mg2+-and homospddeficient cells (Table 3) may offer another aspect of the role of homospd in rhizobial cells, The possible involvement of polyamines in membrane or cell-wall stability has been inferred from many in vitro and in vivo studies (Peter e t al., 1978;Schuber, 1989). In addition to the function of homeostatic buffering, homospd, newly formed in response to the osmotic downshift, might have a protective function for rhizobial cells exposed to low osmotic environments.…”

Section: Discussionmentioning

confidence: 99%

Response of Rhizobium fredii P220 to osmotic shock: Interrelationships between K+, Mg2+, glutamate and homospermidine

Fujihara¹,

Yoneyama²

1994

Microbiology

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The response of Rhizobium fredii P220, a salt-tolerant strain of soybean rhizobia, to osmotic shock was investigated by using non-growing washed cells. Rapid changes in K+, Mg2+, glutamate and homospermidine were observed in strain P220 cells subjected to sudden changes in the osmolarity of incubation buffer. Osmotic upshock resulted in elevation of cellular K+ and glutamate, and reduction in cellular homospermidine and Mg2+. When the cells were transferred to upshock buffer lacking K+, the reduction in Mg2+ was totally blocked, but the elevation of glutamate and the reduction in homospermidine were only partially repressed. Osmotic downshock resulted in the opposite phenomenon: there was an elevation of homospermidine and Mg2+, and a rapid fall of K+ and glutamate. When the cells were transferred to downshock buffer lacking Mg2+, the elevation of homospermidine was partially repressed, but the decrease in K+ and glutamate was not repressed a t all. Lowering of the cellular K+ by treatment with ionophores nigericin and monensin resulted in a slight decrease in glutamate and a slight increase in homospermidine and Mg2+, possibly due to a pH effect caused by the K+-H+ exchange. Raising the cellular Mg2+ content by treatment with ionophore A23187 brought about an increase in homospermidine. The homospermidine content of Mg2+-deficient cells grown with low-Mg2+ medium reduced to 35% of those grown with the basal medium. These results indicate that in R. fredii, K+ strictly controls Mg2+ flux during osmotic shock whereas the reverse is not true, and that glutamate and homospermidine essentially escape direct control by K+. We also suggest that Mg2+, which has no effect on the pool size of glutamate, is one of the factors which regulate homospermidine content in rhizobial cells.

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Section: Discussionmentioning

confidence: 99%

Response of Rhizobium fredii P220 to osmotic shock: Interrelationships between K+, Mg2+, glutamate and homospermidine

Fujihara¹,

Yoneyama²

1994

Microbiology

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“…Furthermore, these water-soluble polyamines are strongly basic, aliphatic multivalent cationic nitrogenated molecule which acquires a highly protonated primary amino groups at physiological pH (Gupta et al 2013(Gupta et al , 2014. Owing to the high affinity of bacterial cells towards these polycationic aliphatic amines, like other cationic synthetic polymers, they may be able to interact with the negatively charged phospholipids (membrane heads) and membrane bound proteins, ion channels, leading to alteration of packing arrangement of the bacterial cells (Schuber 1989). However, there are practically no reports found on usage of putrescine as the capping agent and its advantage through biomimic activity as a better antimicrobial agent mutually along with silver (in nanodimensions).…”

Section: Introductionmentioning

confidence: 99%

Production of putrescine-capped stable silver nanoparticle: its characterization and antibacterial activity against multidrug-resistant bacterial strains

Saha

Gupta²,

Gupta³

et al. 2016

Appl Nanosci

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Integration of biology with nanotechnology is now becoming attention-grabbing area of research. The antimicrobial potency of silver has been eminent from antiquity. Due to the recent desire for the enhancement of antibacterial efficacy of silver, various synthesis methods of silver in their nano dimensions are being practiced using a range of capping material. The present work highlights a facile biomimetic approach for production of silver nanoparticle being capped and stabilized by putrescine, possessing a diameter of 10-25 ± 1.5 nm. The synthesized nanoparticles have been analyzed spectrally and analytically. Morphological studies are carried out by high-resolution transmission electron microscopy and crystallinity by selected area electron diffraction patterns. Moreover, the elemental composition of the capped nanoparticles was confirmed by energy-dispersive X-ray spectroscopy analysis. A comparative study (zone of inhibition and minimum inhibitory concentration) regarding the interactions and antibacterial potentiality of the capped silver nanoparticles with respect to the bare ones reveal the efficiency of the capped one over the bare one. The bacterial kinetic study was executed to monitor the interference of nanoparticles with bacterial growth rate. The results also highlight the efficacy of putrescine-capped silver nanoparticles as effective growth inhibitors against multi-drug resistant human pathogenic bacterial strains, which may, thus, potentially be applicable as an effective antibacterial control system to fight diseases.

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“…It has already been reported that polyamine was associated with acidic phospholipid in the outer membrane of Gram negative bacteria. 35 ) The components of polyamine in the ElM were putrescine, cadaverine, and spermidine. The contents of free type cadaverine and spermidine were 124.8 and 98.0 J.lmoljg, respectively.…”

Section: Resultsmentioning

confidence: 99%

Role of Spermidine in the Ice–Nucleating Activity of the EIM fromErwinia uredovoraKUIN-3

Kawahara

Mano

Hamada

et al. 1994

Bioscience, Biotechnology, and Biochemistry

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Influence of polyamines on membrane functions

Cited by 420 publications

References 153 publications

Response of Rhizobium fredii P220 to osmotic shock: Interrelationships between K+, Mg2+, glutamate and homospermidine

Response of Rhizobium fredii P220 to osmotic shock: Interrelationships between K+, Mg2+, glutamate and homospermidine

Production of putrescine-capped stable silver nanoparticle: its characterization and antibacterial activity against multidrug-resistant bacterial strains

Role of Spermidine in the Ice–Nucleating Activity of the EIM fromErwinia uredovoraKUIN-3

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