Influence of platelet-activating factor, lyso-platelet-activating factor and edelfosine on Langmuir monolayers imitating plasma membranes of cell lines differing in susceptibility to anti-cancer treatment: the effect of plasmalogen level

Abstract

ResearchCite this article: Flasiński M, Ha˛c-Wydro K, Wydro P, Dynarowicz-Ła˛tka P. 2014 Influence of platelet-activating factor, lyso-plateletactivating factor and edelfosine on Langmuir monolayers imitating plasma membranes of cell lines differing in susceptibility to anti-cancer treatment: the effect of plasmalogen level. J. R. Soc. Three structurally related but differing in biological activities single-chained ether phospholipids (PAF (platelet-activating factor) and lyso-PAF) and an anti-cancer drug (edelfosine (ED)) were investigated in Langmuir monolayers imitating natural membranes. The aim of the undertaken experiments was to study the influence of these lipids on monolayers mimicking plasma membranes of cell lines differing in susceptibility to the anti-cancer activity of ED, i.e. promyelocytic leukaemia cells (HL-60) and promyeloblastic leukaemia cells (K-562). As these cells differ essentially in the cholesterol/phospholipid ratio and plasmalogen concentration in the membrane, we have carried out systematic investigations in artificial systems of various compositions. The results for model leukaemia cell membrane were compared with data acquired for systems imitating normal leucocytes. Our results show that the level of plasmalogens significantly modulates the influence of the singlechained phospholipids on the investigated systems. The experiments confirmed also that the interactions of ether lipids with a model membrane of HL-60 cells (in biological tests sensitive to ED) have opposite character when compared with K-562, being resistant to ED. Moreover, the values of the parameters characterizing monolayers serving as membrane models (strength of interactions, monolayers fluidity and morphology) proved both sensitivity of these cells to ED and lack of their susceptibility towards PAF. Interestingly, it has been found that lyso-PAF, which is usually described as an inactive precursor of PAF, displays a stronger effect on HL-60 model membranes than ED.

Structural and biochemical modifications of model and native membranes of human immune cells in response to the action of zinc oxide nanoparticles

“…Therefore, a lot of investigations have been focused on comparing the influence of nanoparticles on membrane systems, both natural and model (Flasiński, Hąc‐Wydro, Wydro, & Dynarowicz‐Łątka, 2014; Huang, Minami, Sakaue, Shingubara, & Takahagi, 2004; Roiter et al, 2008; Zuraw‐Weston et al, 2019). In order to check the effect of ZnO‐NPs on the membranes of HL‐60 and U‐937 immune cells, single‐component lipid systems were examined.…”

“…The excess free energy of mixing (based on the values obtained from mixed ingredients for HL‐60 and U‐937 model monolayers modified by ZnO‐NPs) was calculated, according to the formula of Costin and Barnes (1975) and Flasiński, Hąc‐Wydro, Wydro, and Dynarowicz‐Łątka (2014):…”

Plasmalogen lipids: functional mechanism and their involvement in gastrointestinal cancer

“…The expression of plasmalogens is characteristic for tumorigenicity and an abnormal level of these glycerolipids was identified in various cancerous membranes. Flasinski et al [ 104 ] showed that the addition of ether lipids (PAF, lyso-PAF and edelfosine) destabilizes the films on model membranes with higher choline plasmalogens content (HL-60 and normal + PC-plasma model membranes) and strengthens the interactions in systems lacking choline plasmalogens (normal leucocytes model membrane) or of lower choline plasmalogens level (K-562). It should be pointed out that cell membranes sensitive to edelfosine have a lower level of cholesterol and are enriched by choline plasmalogens (HL-60), while insensitive species have a higher sterol level and simultaneously lack plasmalogens (normal erythrocytes) or are of decreased choline plasmalogens level compared to the sensitive cells (e.g.…”

Membrane affinity and fluorescent labelling: comparative study of monolayer interaction, cellular uptake and cytotoxicity profile of carboxyfluorescein-conjugated cationic peptides

“…To characterise the peptide-lipid interaction monomolecular lipid layers were applied as model membranes. Phospholipid monolayers are extensively used as a model for one leaflet of the cell membrane (Brockman 1999;Więcek et al 2008;Flasinski et al 2014). The surface concentration and charge density of the layer prepared on water surface can be easily adjusted by altering the composition and surface pressure of the monolayer (Kiss et al 2012;Hegedüs et al 2012;Pénzes et al 2012;Schöne et al 2017).…”