Influence of plant origin on propagation capacity and alkaloid biosynthesis during long-term in vitro cultivation of Leucojum aestivum L.

Bogdanova, Y.; Stoeva, Tatyana; Yanev, S.; Pandova, B.; Molle, E.; Burrus, Monique; Stanilova, Marina

doi:10.1007/s11627-008-9178-2

Cited by 14 publications

(9 citation statements)

References 19 publications

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“…Galanthamine levels of some of the in vitro obtained clones was as high as galantamine levels of commercially important representative of Bulgarian L. aestivum populations. Five clones: four galanthamine-type and one lycorine-type were selected as promising for further investigations [140].…”

Section: In Vitro Cultures and Application Of Biotechnology In Gentiamentioning

confidence: 99%

Role of Biotechnology for Protection of Endangered Medicinal Plants

Tasheva¹,

Kosturkova²

2013

Environmental Biotechnology - New Approaches and Prospective Applications

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Section: In Vitro Cultures and Application Of Biotechnology In Gentiamentioning

confidence: 99%

Role of Biotechnology for Protection of Endangered Medicinal Plants

Tasheva¹,

Kosturkova²

2013

Environmental Biotechnology - New Approaches and Prospective Applications

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“…, galanthamine content in L. aestivum directly regenerated bulblets was 10 times greater than in L. aestivum callus‐derived shoots (over 1 mg/g DW vs. 0.1 to 0.2 mg/g DW). According to the same team, genotype is another crucially important factor for the biosynthetic capacity of in vitro cultures . It defines, inter alia, the dominant alkaloid biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Stimulating effect of both 4’‐O‐methylnorbelladine feeding and temporary immersion conditions on galanthamine and lycorine production by Leucojum aestivum L. bulblets

Saliba

Ptak

Boisbrun

et al. 2016

Engineering in Life Sciences

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Bulb cultures of Leucojum aestivum and L. aestivum 'Gravety Giant' were subcultured in medium containing the precursor 4'-O-methylnorbelladine (MN) at various concentrations [0 (control), 0.15 and 0.3 g/L]. The cultures were conducted in bioreactor RITA R and lasted for 15, 30, 40 and 50 days. The growth rate and the alkaloid accumulation in bulblets were studied. For this latter purpose, a purification method was developed. It comprised a highly selective solid phase extraction using on the one hand, UPTI-CLEAN SI and SCX cartridges for plant extracts and on the other hand, 2H cartridges for culture media. Pure alkaloidal fractions were, thus, analyzed by LC-ESI-MS allowing the quantitative evaluation of galanthamine and lycorine from culture extracts. Precursor feeding along with temporary immersion conditions was found to significantly improve the accumulation of both galanthamine and lycorine. The maximal concentrations of galanthamine (0.81 mg/g DW) and lycorine (0.54 mg/g DW) in L. aestivum bulblets were reached, respectively, after 40 days of culture with 0.15 g/L of precursor and after 30 days of culture with 0.3 g/L of precursor. In L. aestivum 'Gravety Giant' bulb cultures, 0.3 g/L of precursor was the best condition for both galanthamine (0.6 mg/g DW after 50 days) and lycorine (1.13 mg/g DW after 30 days).

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“…The present study demonstrates that a micropropagation system, not involving the production of embryogenic calli, can also be established for this species. Besides our report, several studies reported the production of L. aestivum tissue cultures [7,26], but none of them involved the assay of antioxidant capacities (see below). All cultures showed high shoot / whole plant and (except L. aestivum) high bulblet regeneration frequencies (Tab.…”

Section: Discussionmentioning

confidence: 94%

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

et al. 2017

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The aim of this study was (i) to produce tissue cultures capable of efficient plant regeneration from European naturally occurring protected and/or pharmacologically important Amaryllidaceae species and (ii) to test them for antioxidant activities in order to select tissue cultures that scavenge efficiently oxygen radicals. Bulb explants were collected from Galanthus woronowii, two Leucojum species, and Sternbergia lutea. Leucojum species were Hungarian isolates. Mostly α-naphthalene acetic acid (NAA) and benzyladenine (BA) were used as growth regulator combinations for the induction and maintenance of tissue cultures and further antioxidant activity studies. Galanthus woronowii and L. vernum cultures produced shoots or whole plants via micropropagation (callus stage was observed only sporadically and callus tissue did not contribute to regeneration), whereas L. aestivum and S. lutea produced efficiently whole plants or multiple shoots via embryogenic calli. Total phenolic content, % inhibition of ABTS radical (ABTS*) cation, and peroxidase activities on native polyacrylamide gels were studied and showed differences between cultures. No relationship could be detected between polyphenol content / radical scavenging capacities and H 2 O 2 reducing enzyme activities. For G. woronowii, S. lutea, and a culture line of L. vernum, polyphenol content and ABTS* cation scavenging activities were high and for G. woronowii, comparable to organs of the native plants used as explant sources. Bulbs of native plants showed low radical scavenging activities in general. For L. vernum and L. aestivum tissue cultures grown in the presence of NAA as the sole growth regulator, ABTS* cation scavenging showed low values. Enzymatic antioxidant (pyrogallol peroxidase) activities were low for all cultures and organs of native plants. This study shows the species conservation value of these cultures and highlights the high antioxidant capacity of G. woronowii and S. lutea, attributed to the presence of non-enzymatic scavengers.

show abstract

Influence of plant origin on propagation capacity and alkaloid biosynthesis during long-term in vitro cultivation of Leucojum aestivum L.

Cited by 14 publications

References 19 publications

Role of Biotechnology for Protection of Endangered Medicinal Plants

Role of Biotechnology for Protection of Endangered Medicinal Plants

Stimulating effect of both 4’‐O‐methylnorbelladine feeding and temporary immersion conditions on galanthamine and lycorine production by Leucojum aestivum L. bulblets

Production and antioxidant capacity of tissue cultures from four Amaryllidaceae species

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