Influence of plant growth regulators on indirect shoot organogenesis and secondary metabolite production in Aconitum violaceum Jacq.

Abstract

Influence of plant growth regulators on indirect regeneration and secondary metabolite production in Aconitum violaceum Jacq. was evaluated. Among the different plant growth regulators studied, 2.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.25 µM kinetin (Kn) promoted the highest frequency of callus production for indirect regeneration. 6-Benzyl aninopurine (BAP) was more effective in improving shoot regeneration and secondary metabolite production compared to thidiazuron (TDZ). The highest frequency of regeneration (61.8%) was obtained when calli were transferred to Murashige and Skoog medium supplemented with 1 µM BAP and 0.5 µM α-naphthalene acetic acid (NAA) and was more than two-times higher when compared to the treatments with cytokinin only. Supplementation with low NAA concentrations resulted reduction in in vitro secondary metabolite production in most cases, when compared to treatments with cytokinin only. Moreover, differences in cytokinin concentrations significantly affected secondary metabolite production in some cases. The current findings highlighted the differential effects of auxin-cytokinin interactions on indirect shoot regeneration and the production of secondary metabolites in A. violaceum.

Schisandra henryi C. B. Clarke in vitro cultures: a promising tool for the production of lignans and phenolic compounds

“…NAA significantly increased shoot proliferation and influenced tannins, flavonoids and phenols accumulation in Huernia hystrix in vitro cultures (Amoo and van Staden 2013). In Aconitum violaceum shoot culture differences in cytokinin (BA, Kin, TDZ) concentrations, and longer durations of growth periods (8 weeks) significantly affected aconitine production (Janhvi et al 2013). In Stevia rebaudiana tissue the increase of total phenols and flavonoids production varied with PGRs and BA either with GA 3 or IAA gave best results (Radić et al 2016).…”

Plant Secondary Metabolite Transporters: Diversity, Functionality, and Their Modulation

“…Similarly, fungal oligosaccharide when applied to the roots of M. truncatula could elevate the mRNA transcripts of MtABCG10, which plays an important role in defense response by modulating medicarpin biosynthesis in the roots of the plant ( Banasiak et al, 2013 ). In addition, various plant growth regulators such as cytokinins, auxins, brassinosteroids, ethylene, and gibberellins are also reported to have a notable impact on the biosynthesis and accumulation of various nutrients or metabolites which in turn, might affect the functioning of corresponding transporters ( Rawat et al, 2013 ; Jamwal et al, 2018 ). One such example is cytokinin-mediated substantial suppression of macronutrient (ammonium, nitrate, sulfate, and phosphate) transporters ( Sakakibara et al, 2006 ).…”