Influence of physical activity of racehorses on lactate dehydrogenase and creatine kinase activities, and protein synthesis

Abstract

The aim of the research was to assess the effects of physical activity of various intensity on the degree of damage to certain organs resulting from increased free radical production, as well as the adaptability of the organism to physical exercise.Two groups of healthy 3-5-year-old full-blooded racehorses were assessed. The first one ran a 2400-meter gallop race, which is considered a short-lasting, intense physical activity; lipid status was assessed prior to, and 48 and 72 h after the race. The second group ran a forty-kilometer endurance ride, which is a long-lasting moderate physical activity; the lipid status was assessed immediately before, on finishing and 48, 72, 96, 120 and 144 h after the race. The total activity of LDH changed 72 h and 96 h following the gallop race (p>0.05), whilst the maximum activity was measured immediately after the endurance ride. By means of electrophoresis LDH in all the horses 5 isoforms were detected. The activity of LDH1 72 h after the gallop race significantly rose in comparison to the one before the race (p<0.05), whilst the activity of all other isoenzymes, LDH2-LDH5, did not change significantly (p>0.05). Following the endurance ride LDH1 activity rose at all sampling times, reaching the maximum at 96 h and 144 h in comparison to the values both before and on finishing the ride (p<0.01). The increase in the activity of LDH2 was significantly higher 48 h, 72 h, 96 h and 120 h (p<0.05) after the race in comparison to that before the race, and at 48 h, 72 h, 96 h, 120 h and 144 h (p<0.05) in comparison to the values on finishing the race. LDH3 activity significantly decreased and LDH5 rose immediately after the endurance ride (p<0.01), whilst LDH4 significantly rose at all times following the endurance ride (p<0.01). The CK activity pointed to high, medium and low degree of adaptation of horses to physical activity. 550The concentrations of total proteins, albumins and globulins remained within the physiological range at all sampling times, with the exception of 96 h after the endurance ride, when the fall in albumin concentration was significant (p<0.01).Long-lasting physical activity of low intensity leads to cellular damage in the myocardium, muscles, liver and to hypoalbuminaemia, which is a consequence of free radical production.

Oxidative stress and DNA damage in horses naturally infected with Theileria equi

“…The appearance of a cellular enzyme such as LDH in the extracellular space is a useful marker of disrupted cellular integrity. There are five forms of LDH (isoenzymes LDH 1 -LDH 5 ) and the distribution of each isoenzyme varies between tissues (Jović et al, 2013). We observed that only LDH 5 , related to liver damage, was significantly increased in Theileria infected horses.…”

Implications of oxidative stress in occupational exposure to lead on a cellular level

“…The increased activity of LDH found in plasma of workers in this study were probably an outcome of cellular membrane destruction due to increased peroxidation .The distribution of LDH isoenzymes can indicate the potential damage to certain organs (Jović et al 2013), according to their tissue specificity. The revealed LDH 1-5 isoenzyme distribution showed variations in results among two groups, depending on the degree of oxidative damage in each tissue.…”

Efectos de dos protocolos de entrenamiento sobre el lactato sanguíneo en caballos de paso fino

“…Varios autores han descrito el aumento de la CK pos-ejercicio ejercicio por el aumento de la permeabilidad del sarcolema (Piccione et al 2009;Jović et al 2013;Mami et al 2019). Según Piccione et al (2009), los niveles de CK se relacionan con el estado de entrenamiento.…”