INFLUENCE OF PHOSPHATE CONCENTRATIONS ON GLUCOAMYLASE PRODUCTION BY Aspergillus awamori IN SUBMERGED CULTURE

Zaldivar-Aguero, J.M.; Badino, Alberto Colli; Vilaça, Paulo; Facciotti, Maria Cândida Reginato; Schmidell, Willibaldo

doi:10.1590/s0104-66321997000400014

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Similar results were obtained by [17]. Moreover, [42] reported that the synthesis of a-amylase is stimulated by adding phosphorus to the culture medium and the concentration recommended is 7.0-10.0 g/l.…”

Section: ) Production Of A-amylasesupporting

confidence: 87%

Optimization of ethanol, citric acid, and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger, and Candida guilliermondii

Acourene¹,

Ammouche²

2012

Journal of Industrial Microbiology and Biotechnology

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The present study deals with submerged ethanol, citric acid, and α-amylase fermentation by Saccharomyces cerevisiae SDB, Aspergillus niger ANSS-B5, and Candida guilliermondii CGL-A10, using date wastes as the basal fermentation medium. The physical and chemical parameters influencing the production of these metabolites were optimized. As for the ethanol production, the optimum yield obtained was 136.00 ± 0.66 g/l under optimum conditions of an incubation period of 72 h, inoculum content of 4% (w/v), sugars concentration of 180.0 g/l, and ammonium phosphate concentration of 1.0 g/l. Concerning citric acid production, the cumulative effect of temperature (30°C), sugars concentration of 150.0 g/l, methanol concentration of 3.0%, initial pH of 3.5, ammonium nitrate concentration of 2.5 g/l, and potassium phosphate concentration of 2.5 g/l during the fermentation process of date wastes syrup did increase the citric acid production to 98.42 ± 1.41 g/l. For the production of α-amylase, the obtained result shows that the presence of starch strongly induces the production of α-amylase with a maximum at 5.0 g/l. Among the various nitrogen sources tested, urea at 5.0 g/l gave the maximum biomass and α-amylase estimated at 5.76 ± 0.56 g/l and 2,304.19 ± 31.08 μmol/l/min, respectively after 72 h incubation at 30°C, with an initial pH of 6.0 and potassium phosphate concentration of 6.0 g/l.

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Section: ) Production Of A-amylasesupporting

confidence: 87%

Optimization of ethanol, citric acid, and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger, and Candida guilliermondii

Acourene¹,

Ammouche²

2012

Journal of Industrial Microbiology and Biotechnology

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“…Their concentration in the medium and type of source (inorganic or organic) can stimulate or inhibit the laccase production. Effect of phosphate on glucoamylase production showed a positive correlation . It is known that high concentration of ATP leads to enzyme secretion in fungi .…”

Section: Resultsmentioning

confidence: 87%

“…Effect of phosphate on glucoamylase production showed a positive correlation. 29 It is known that high concentration of ATP leads to enzyme secretion in fungi. 30 Laccase production media that are already reported for prokaryotes coincidently contain rarely more than 10 mM of inorganic phosphate.…”

Section: Resultsmentioning

confidence: 99%

Impact of phosphate and other medium components on physiological regulation of bacterial laccase production

Kaur

Singh

Gupta

et al. 2016

Biotechnology Progress

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Laccases are multicopper oxidases known to catalyze the transformation of a wide range of phenolic and non-phenolic substrates using oxygen as electron acceptor and forming water as the only by product. Their potential relevance in several industries requires the constant search for novel laccases. Positive outcome of the isolation of laccase producing bacteria depends on the nature and concentration of media constituents. Several attempts to isolate laccase producing bacteria failed when the phosphate-containing M9 minimal medium was used. Shift to phosphate-less M162 medium led to successful isolations. Seven bacterial isolates belonging to genera Bacillus, Lysinibacillus, Bhargavaea and Rheinheimera were used to study the effect of medium constituents on laccase production. Inorganic phosphate (≥50 mM) was found to regulate laccase synthesis negatively though no inhibitory effect of phosphate (10-500 mM) was seen on laccase activity. All isolates ceased laccase synthesis when grown in the presence of tryptone (0.2-1%), with R. tangshanensis as an exception, or yeast extract (1.5-2%) as the only C/N source in M162 medium. Supplementation upto 0.1% of glucose in basal M162 medium increased laccase production in five isolates but decreased at higher concentrations. The influence of medium components on laccase synthesis was further affirmed by zymographic studies. These observations offer possibilities of isolating promising laccase producers from diverse environmental sources. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:541-548, 2017.

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“…Moreover, Zaldivar-Aguero et al, (40) reported that the synthesis of α-amylase is stimulated by adding 7.0 to 10.0 g/L phosphorus to the culture medium. In contrast, Jin et al, (30) did not obtain a significant improvement in gluco-amylase yields by adding phosphorus to the fermentation medium.…”

Section: Optimization Of α-Amylase Productionmentioning

confidence: 99%

Date Wastes as Substrate for the Production of α-Amylase and Invertase

Said

Leila²,

Kaouther

et al. 2014

Iran J Biotech

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Background In Algeria, the production of dates is estimated to be 520.00 tons of which 85.000 to 105.000 tons are of low commercial value (1). Date wastes is considered as an economical source of carbohydrates for conversion to industrial enzymes because it is readily available and relatively low priced. Several extra cellular enzymes are commercially available and widely used in the industry. Among them, α-amylases and invertases are used in many industrial processes. For example, αamylases are involved in the assimilation of starch-based matter provided as substrates. Amylases find potential application in a number of industrial processes such as food processing, fermentation, textile, paper industries, and in biotechnology (2). These enzymes are produced by a variety of microorganisms such as Aspergillus niger, A. awamori, A. oryzae, A.

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INFLUENCE OF PHOSPHATE CONCENTRATIONS ON GLUCOAMYLASE PRODUCTION BY Aspergillus awamori IN SUBMERGED CULTURE

Cited by 7 publications

References 6 publications

Optimization of ethanol, citric acid, and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger, and Candida guilliermondii

Optimization of ethanol, citric acid, and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger, and Candida guilliermondii

Impact of phosphate and other medium components on physiological regulation of bacterial laccase production

Date Wastes as Substrate for the Production of α-Amylase and Invertase

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