Influence of pH on the inhibitory effects of local anesthetics on histamine release induced from rat mast cells by concanavalin A and compound

Comparison of histamine release induced by synthetic polycations with that by compound 48/80 from rat mast cells.

“…Values are given as rneans±S.E. *P<0.05, **P<0 .01, ***P<0.001. cells, like the case of the release induced by compound 48/80 (10). Its effects on mast cells did not change the histamine release induced by polycations with various degrees of polymerization (Fig.…”

“…However, lidocaine inhibits the histamine release in duced by calcium ionophore A23187 when there is enough intracellular calcium to trigger histamine releases, suggesting that their in hibitory actions on histamine release induced by A.23187 cannot be explained by their effect on calcium influx (14). Our group previously reported that the inhibitory effects of lidocaine on mast cells was correlated with the concen tration of nonionized molecules which pene trate the mast cell membrane (10). The site of action for lidocaine on mast cells is still unknown, but !idocaine may interfere with steps in the release cascade that occurred after opening of calcium channels, such as calmodulin-dependent ones (15).…”

“…There are three types of histamine release inhibitors from rat mast cells: specific inhibi tors of histamine release mediated by IgE re ceptors, such as methoxy verapamil (D-600, a calcium antagonist) and disodium cromo glycate (DSCG) (4,5); specific inhibitors of release induced by compound 48/80, such as islet activating protein (lAP) (6,7); and in hibitors of both histamine releases mediated by IgE receptors and compound 48/80, such as dibutyryl cyclic AMP and local anesthetics (8)(9)(10). The histamine releases induced by PEIs and PAAs were inhibited by dibutyryl cyclic AMP, but not by D-600, and D-600 did not block the increase in intracellular calcium concentrations by polycations (2).…”

Beyond a Simple Anesthetic Effect: Lidocaine in the Diagnosis and Treatment of Interstitial Cystitis/bladder Pain Syndrome

“…Subsequent investigation of this phenomenon showed that lidocaine induced a strong inhibition of activated mast cell histamine release, with increasing effect at higher pH. 44 LAs have also been shown to competitively inhibit histamine-1 receptors and the release of histamine in skin by platelet-activating factor. 43 Another possibly relevant but less-known property of these drugs is a broad-spectrum bactericidal effect that is also concentration dependent.…”

Effect of Proparacaine in a Mouse Model of Allergic Rhinitis

“…Lidocaine is known to have significant effects on nonexcitable cells, including neutrophils, monocytes, Th2 lymphocytes, mast cells, and eosinophils [5][6][7][8][9][10][11]. A previous study found that local anesthetics, such as lidocaine and it analogues reduced eosinophil response to cytokines which resulted in immune modulatory effects [5].…”