Influence of particulate and dissociated metal-on-metal hip endoprosthesis wear on mesenchymal stromal cells in vivo and in vitro

Rakow, Anastasia; Schoon, Janosch; Dienelt, Anke; John, Thilo; Textor, Martin; Duda, Georg N.; Perka, Carsten; Schulze, Frank; Ode, Andrea

doi:10.1016/j.biomaterials.2016.04.023

Cited by 62 publications

(42 citation statements)

References 43 publications

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“…Cellular models can be employed to characterize in vitro the behavior of different biomaterials that are used as bone substitutes (4)(5)(6)(7)(8). In our study, human mesenchymal stem cells (hMSCs) that were obtained from bone marrow aspirates were used as a cellular model of study to analyze their behavior when grown on different biomaterials (4,8).…”

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confidence: 99%

Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold

et al. 2017

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Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaffold, osteogenic genes were analyzed for their expression profiles, together with adhesion and extracellular matrix genes. In hASC integrins, basement membrane constituents and collagens were up-regulated, together with cell proliferation. In addition, expression of osteopontin and activated focal adhesion kinase was studied at the protein level. Our data indicate that hASCs, together with hybrid biomaterial, is an important model of study to investigate bone induction.-Mazzoni, E., D'Agostino, A., Manfrini, M., Maniero, S., Puozzo, A., Bassi, E., Marsico, S., Fortini, C., Trevisiol, L., Patergnani, S., Tognon, M. Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold.

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confidence: 99%

Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold

et al. 2017

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“…101 Rakow et al determined the concentrations of dissociated Co 21 and Cr 31 ions in peri-implant tissues surrounding failed metal-on-metal (MoM) implants and exposed cultured human MSCs to a range of clinically relevant metal ion concentrations. 102 A dose-dependent reduction in MSC viability, proliferation, ALP expression, and matrix mineralization was documented. Interestingly cobalt has been shown to induce a hypoxia-like response in MSCs and macrophages by stabilizing HIF-1a.…”

Section: Impact Of Wear Debris On Mscsmentioning

confidence: 96%

“…Rakow et al isolated MSCs from bone marrow surrounding revised MoM implants. 102 High metal ion and particle concentration in the tissues surrounding the implant was demonstrated and subsequently the revision-derived MSCs displayed reduced osteogenic potential with diminished ALP activity; interestingly reduced ALP levels were detected also from the patients' circulation. MSC viability, proliferation, and migration as well as chrondro-and adipogenic differentiation ability were intact.…”

Section: Retrieval Studiesmentioning

confidence: 99%

“…Schröck et al observed that soluble Co2+ ions inhibited osteogenic differentiation of human MSCs in a dose‐dependent manner . Rakow et al determined the concentrations of dissociated Co 2+ and Cr 3+ ions in peri‐implant tissues surrounding failed metal‐on‐metal (MoM) implants and exposed cultured human MSCs to a range of clinically relevant metal ion concentrations . A dose‐dependent reduction in MSC viability, proliferation, ALP expression, and matrix mineralization was documented.…”

Section: Mesenchymal Stem Cells In Aseptic Looseningmentioning

confidence: 99%

“…Importantly, both the osteogenic and adipogenic differentiation ability of revision MSCs was diminished. Rakow et al isolated MSCs from bone marrow surrounding revised MoM implants . High metal ion and particle concentration in the tissues surrounding the implant was demonstrated and subsequently the revision‐derived MSCs displayed reduced osteogenic potential with diminished ALP activity; interestingly reduced ALP levels were detected also from the patients' circulation.…”

Section: Mesenchymal Stem Cells In Aseptic Looseningmentioning

confidence: 99%

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Mesenchymal stem cells in the aseptic loosening of total joint replacements

Pajarinen

Lin

Nabeshima

et al. 2017

J Biomedical Materials Res

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Peri-prosthetic osteolysis remains as the main long-term complication of total joint replacement surgery. Research over four decades has established implant wear as the main culprit for chronic inflammation in the peri-implant tissues and macrophages as the key cells mediating the host reaction to implant-derived wear particles. Wear debris activated macrophages secrete inflammatory mediators that stimulate bone resorbing osteoclasts; thus bone loss in the peri-implant tissues is increased. However, the balance of bone turnover is not only dictated by osteoclast-mediated bone resorption but also by the formation of new bone by osteoblasts; under physiological conditions these two processes are tightly coupled. Increasing interest has been placed on the effects of wear debris on the cells of the bone-forming lineage. These cells are derived primarily from multipotent mesenchymal stem cells (MSCs) residing in bone marrow and the walls of the microvasculature. Accumulating evidence indicates that wear debris significantly impairs MSC-to-osteoblast differentiation and subsequent bone formation. In this review, we summarize the current understanding of the effects of biomaterial implant wear debris on MSCs. Emerging treatment options to improve initial implant integration and treat developing osteolytic lesions by utilizing or targeting MSCs are also discussed.

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Metal‐Specific Biomaterial Accumulation in Human Peri‐Implant Bone and Bone Marrow

et al. 2020

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Metallic implants are frequently used in medicine to support and replace degenerated tissues. Implant loosening due to particle exposure remains a major cause for revision arthroplasty. The exact role of metal debris in sterile peri-implant inflammation is controversial, as it remains unclear whether and how metals chemically alter and potentially accumulate behind an insulating peri-implant membrane, in the adjacent bone and bone marrow (BM). An intensively focused and bright synchrotron X-ray beam allows for spatially resolving the multi-elemental composition of peri-implant tissues from patients undergoing revision surgery. In peri-implant BM, particulate cobalt (Co) is exclusively co-localized with chromium (Cr), non-particulate Cr accumulates in the BM matrix. Particles consisting of Co and Cr contain less Co than bulk alloy, which indicates a pronounced dissolution capacity. Particulate titanium (Ti) is abundant in the BM and analyzed Ti nanoparticles predominantly consist of titanium dioxide in the anatase crystal phase. Co and Cr but not Ti integrate into peri-implant bone trabeculae. The characteristic of Cr to accumulate in the intertrabecular matrix and trabecular bone is reproducible in a human 3D in vitro model. This study illustrates the importance of updating the view on long-term consequences of biomaterial usage and reveals toxicokinetics within highly sensitive organs.

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Influence of particulate and dissociated metal-on-metal hip endoprosthesis wear on mesenchymal stromal cells in vivo and in vitro

Cited by 62 publications

References 43 publications

Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold

Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold

Mesenchymal stem cells in the aseptic loosening of total joint replacements

Metal‐Specific Biomaterial Accumulation in Human Peri‐Implant Bone and Bone Marrow

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