Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold

Abstract

Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaffold, osteogenic genes were analyzed for their expression profiles, together with adhesion and extracellular matrix genes. In hASC integrins, basement membrane constituents and collagens were up-regulated, together with cell proliferation. In addition, expression of osteopontin and activated focal adhesion kinase was studied at the protein level. Our data indicate that hASCs, together with hybrid biomaterial, is an important model of study to investigate bone induction.-Mazzoni, E., D'Agostino, A., Manfrini, M., Maniero, S., Puozzo, A., Bassi, E., Marsico, S., Fortini, C., Trevisiol, L., Patergnani, S., Tognon, M. Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold.

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

“…These cells are positive for surface markers CD13, CD29, CD44, CD73, CD90, and CD105, CD166, whereas they are negative for other markers, such as CD14, CD31, and CD45. Cells were expanded in Dulbecco's Modified Eagle Medium F‐12 (DMEM/F12; Lonza, Milan, Italy), as previously described . Primary hASC cultures were grown (i) in the presence of the osteogenic condition (OC) only; (ii) on the biomaterial Coll/ Pro Osteon 200; (iii) in the tissue culture polystyrene (TCPS) vessels, the control .…”

“…Cells were expanded in Dulbecco's Modified Eagle Medium F‐12 (DMEM/F12; Lonza, Milan, Italy), as previously described . Primary hASC cultures were grown (i) in the presence of the osteogenic condition (OC) only; (ii) on the biomaterial Coll/ Pro Osteon 200; (iii) in the tissue culture polystyrene (TCPS) vessels, the control . Experimental time points were at days 14, 21, and 40 as indicated for the different assays later in the article.…”

“…Porous HA‐derived scaffold used herein is composed by Granular Pro Osteon200 (Interpore Cross Irvine, California) and Avitene Microfibrillar Collagen Hemostat (Bard Warwick, Rhode Island) (Coll) . This scaffold is named Coll/Pro Osteon 200.…”

“…Granular Pro Osteon 200 is a coralline HA, which is very similar in its make up to human bone mineral composition and form. The manufacturing scaffold using in vitro and in vivo evaluations was descried before in detail …”

“…In a previous study gene expression analyses by RT‐PCR technology were carried out at early times post‐cell seeding on biomaterial. To this end, mRNAs extracted from cells, hASCs, were comparatively analyzed by RT‐PCR at days 3 and 9. hASCs were grown on the scaffold, and plastic vessel (TCPS), used as control.…”