Influence of Organic Supplements on Growth and Development of &lt;i&gt;In&lt;/i&gt; &lt;i&gt;Vitro&lt;/i&gt; Shoots of &lt;i&gt;Bulbophyllum dhaninivatii&lt;/i&gt; Seidenf

Kongbangkerd, Anupan; Watthana, Santi; Srimuang, Kanok Orn

doi:10.4028/www.scientific.net/amm.855.42

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…The addition of CW to the VW medium, together with potato and banana homogenate, had a significant impact on shoot regeneration and plantlet development. Organic growth additives can promote the production and multiplication of B. Dhaninivatii shoots [19]. Minea et al (2004) [20] studied the impact of 10% banana homogenate (BH) on the size of Spathoglottis kimballianai leaves.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it promoted Calopogon tuberosus seed germination and improved protocorm growth [26]. [19] examined B. dhaninivatii shoots cultivated in vitro for 12 weeks on semi solid VW medium, which was supplemented with different concentrations of a mixture of coconut water, potato extract (PE), and banana homogenate (BH). They stated that adding organic supplements to the medium resulted in higher in vitro shoot development and morphological improvement over those cultures.…”

Section: Gnasekaranmentioning

confidence: 99%

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Effect of Culture Medium, Growth Regulators and Organic Additives on Shoot Regeneration from Hybrid-Seeds of Phalaenopsis

Tram,

Hoa,

Diep

et al. 2024

Biotechnol. J. Int.

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Aim: The Phalaenopsis Orchid, also known as the Moth Orchid, is a magnificent multicolored big orchid that has become a popular indoor plant and is easily recognized nowadays. The study of identifying the effects of culture medium, growth regulators, and organic additives on shoot growth from regenerated hybrid-seeds of Phalaenopsis is required in the first three months. Methodology: Shoots from hybrid-seeds of Phalaenopsis germination after 2 months were used as materials. Experiments were designed with the effects of nutrition mediums (MS and VW), BAP, and organic additives in Randomized Complete Block Design (CRD) via evaluation parameters on shoot growth (via number of leaves) and root growth (via root number and root length). Results: In Experiment 1, after 12 weeks, the number of leaves per shoot on 1/2xMS medium increased from 3.45 to 4.50; and root growth performed better in 1/2xMS medium (4.06 cm). In experiment 2, 1/2xMS medium + 0.05 mg/L BAP showed the highest number of roots per shoot (4.80 roots per shoot). 1⁄2xMS medium and VW medium without BAP showed the longest roots length (4.06 cm and 4.18 cm, respectively). In experiment 3, VW medium + potato showed the highest number of leaves per shoot (8.95 leaves per shoot) and VW medium + coconut water (CW) showed the longest roots length (4.78 ± 0.09 cm). in experiment 4, root growth performed better in medium enriched with CW (4.80 roots per shoot and root length 2.69 cm). Conclusion: Both 1/2xMS and VW medium are necessary for shoot from hybrid-seeds growth in the first 6-weeks and VW medium is favored for development of shoot and root after 6-weeks.

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Section: Discussionmentioning

confidence: 99%

Section: Gnasekaranmentioning

confidence: 99%

Effect of Culture Medium, Growth Regulators and Organic Additives on Shoot Regeneration from Hybrid-Seeds of Phalaenopsis

Tram,

Hoa,

Diep

et al. 2024

Biotechnol. J. Int.

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“…In vitro protocorm proliferation of the species of Cymbidium pendulum was previously studied using organic growth supplements [5], An epiphytic orchid Bulbophyllum nipondhii Seidenf [3], Bulbophyllum dhaninivatii Seidenf [11].…”

Section: Role Of Orchidsmentioning

confidence: 99%

“…Addition of 100ml of coconut water to the VW medium, along with 50ml of potato extract and 50ml of banana homogenate had the greatest impact on shoot regeneration and formation of the plantlets. Organic growth supplements can encourage B. Dhaninivatii shoots formation and multiplication [11].…”

Section: Micro Propagationmentioning

confidence: 99%

“…Kongbangkerd et al [11] reviewed on B. dhaninivatii shoots which were cultured in vitro multiplication for 12 weeks on semi-solid VW medium, in which supplemented with varying concentration of a mixture of coconut water with Potato extract (PE) and also banana homogenate (BH). They mentioned that applying organic supplements to the medium, increased in vitro shoots growth and morphological improvement over those culture.…”

Section: Micro Propagationmentioning

confidence: 99%

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Role of Organic Growth Supplement In vitro Multiplication of Orchid Species- A Review

Momtaj

Sushma

Kaur

2021

JPRI

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The main purpose of this article is to review role of several organic growth additives such Apple juice, coconut water (CW), maize extract, banana homogenate (BH), peptone and protocorms etc which stimulate the multiplication rate of various orchid species in in vitro multiplication. These organic growth supplements help to increase the number of shoots, root and leaf in culture medium. In many orchid tissue culture, organic growth supplements, which are the most essential medium aspect to stimulate tissue growth, production and facilitate the regeneration of shoot. The banana homogenate (BH) had the highest rate of regeneration and root developments. The use of organic growth supplements resulted in increased regeneration, the creation of more shoots and the development of fresh plantlets. Amino acids, proteins, vitamins, carbohydrates and various types of organic compounds are present in these growth supplements. These components have the potential to play a significant role in the development and creation of culture. Now more research is needed to figure out which factors are responsible for the organic additives’ promoter effect.

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Orchid Seed Germination and Micropropagation II: Media Information and Composition

Park

Yeung

2018

Springer Protocols Handbooks

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Influence of Organic Supplements on Growth and Development of <i>In</i> <i>Vitro</i> Shoots of <i>Bulbophyllum dhaninivatii</i> Seidenf

Cited by 7 publications

References 20 publications

Effect of Culture Medium, Growth Regulators and Organic Additives on Shoot Regeneration from Hybrid-Seeds of Phalaenopsis

Effect of Culture Medium, Growth Regulators and Organic Additives on Shoot Regeneration from Hybrid-Seeds of Phalaenopsis

Role of Organic Growth Supplement In vitro Multiplication of Orchid Species- A Review

Orchid Seed Germination and Micropropagation II: Media Information and Composition

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