The present work investigates pollutant removal and the transformation of nitrogen from sewage wastewater using a pilot-scale multi-stage bio-vermifilter system. Over a study period of 48 weeks, the pollutant removal performance of the system was measured and the effects of hydraulic loading rate (HLR) and dry-wet ratio (D/W) were estimated. The relationship between oxygen transfer rate and load of oxygen necessity was calculated and analysed for system optimisation. The method for diluting the isotope d 15 N-NO 3 -was applied to study nitrogen transfer. Moreover, statistical correlations were analysed to determine the crucial factors which influence nitrogen transfer efficiency. The system removes pollutants efficiently; specifically, the average removal efficiencies are 94.2 % for chemical oxygen demand (COD), 93.3 % for NH 4 ? -N, and 58.2 % for total nitrogen (T-N). Lowering HLR and D/W can enhance nitrogen removal. Nitrogen speciation and transformation were examined under an optimised condition with an HLR of 0.36 m day -1 and a D/W of 3. The results of isotope d 15 N-NO 3 -dilution showed that NO 3 --N was mainly produced in trickling bio-filter and vermibio-filter (VBF)