Influence of nutrition on the production and physiology of sectors produced by the insect pathogenic fungusMetarhizium anisopliae

Abstract

Metarhizium anisopliae strains V245 and V275 differed in their stability when grown on different nutrient media. V275 produced fewer sectors than V245 irrespective of the cultural conditions. Both strains produced more sectors on nutrient rich media. At least four distinct types of sectors were produced in vitro. Most sectors were sterile or sporulated poorly and produced significantly lower quantities of virulence determining enzymes like Pr1. Real-time PCR confirmed differential expression of the pathogenicity-related genes pr1 A, ste 1, try 1, and chy 1 encoding for the subtilisin Pr1A, esterase, trypsin and chymotrypsin, respectively. API-ZYM revealed that the enzyme profiles of sectors differed from those of the parent cultures and also from other sectors. Sectors of M. anisopliae also produced less destruxins than the parent cultures independent of the strain.

Associated links among mtDNA glycation, oxidative stress and colony sectorization in Metarhizium anisopliae

“…NADPH oxidase, producing ROS that trigger apoptosis in mammalian cell-lines (Li and Renier, 2006). Caloric restriction (CR) increases the life span of many organisms including yeasts, at least in part by reducing oxidative stress (Castello et al, 2005), and low sugar levels significantly reduce sectorization in Metarhizium (Shah and Butt, 2005). This is consistent with AGEs exerting their effect upstream of ROS accretion.…”

“…This is true of the insect pathogenic fungus Metarhizium anisopliae (Ryan et al, 2002;Shah and Butt, 2005;. M. anisopliae is a well studied model for understanding fungal-insect interactions St.…”

Virulence of Hypocreales fungi to pecan aphids (Hemiptera: Aphididae) in the laboratory

“…Blastospores were produced in a liquid medium whereas conidia were produced on solid medium; both media were comprised of a different nutritional background. Nutritional content is known to affect the production quality of various Hypocreales species (Shah and Butt, 2005;Vidal et al 1998). We justified the choice of spore types tested based on what is currently available or being pursued for commercial application.…”

Literature search and data collection on RA for human health for microorganisms used as plant protection products

“…The production of toxic metabolites by fungi was shown to depend on growth conditions, such as temperature (Apetroaie-Constantin et al, 2009;From et al, 2007), atmospheric composition (Apetroaie-Constantin et al, 2009), and the pH and composition of the growth medium (Shah and Butt, 2005;Wang et al, 2004).…”