Influence of nitrilotriacetic acid on Cd2+ uptake by yeast

Ahlers, J.; Rösick, Erika

doi:10.1007/bf01607735

Cited by 6 publications

(1 citation statement)

References 14 publications

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“…by the presence of NTA. 12 Moreover, it is evident that the cadmium concentrations permitted in waste water by environmental acts would have a drastic effect on the growth of yeast and possibly also of other microorganisms. Since the main aim of our project was to discover, whether the toxic effect of environmental chemicals may at least in part be due to an interaction with the membrane, we looked for a correlation between effects on cell growth and on integral membrane proteins.…”

Section: Discussionmentioning

confidence: 99%

Cadmium‐uptake and influence on plasma membrane functions of yeast†

Ahlers¹,

Rösick²

1986

Toxicological & Environmental Chemistry

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115m Cd 2+ -uptake and accumulation by the yeast Saccharomyces cerevisiae was rapid and largely dependent on the presence of glucose. From the time response after addition of glucose and from studies with energy poisons or with cycloheximide, it could be deduced that the observed effects were neither due to a cotransport system of glucose and Cd 2+ nor due to the biosynthesis of binding or transport proteins, but to an energy-dependent transport system (K m :0.3 mM, V max :5n mol/min x mg dry wt), being driven by the electrochemical potential across the plasma membrane.There was a good correlation between the influence of cadmium on the kinetics of cell growth and toxic effects of Cd 2+ on the plasma membrane H + -ATPase. This inhibition was strongly dependent on the experimental conditions. Cadmium ions were most effective at high pH and high magnesium concentrations. They enhanced K m , whereas V max was not affected significantly. Under favourable conditions we found a lowest effective concentration of Cd 2+ at <1μM and 50% inhibition at 3.5 ΜM. In contrast, CdCl 2 had no effect on glucose and purine transport systems.

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Section: Discussionmentioning

confidence: 99%

Cadmium‐uptake and influence on plasma membrane functions of yeast†

Ahlers¹,

Rösick²

1986

Toxicological & Environmental Chemistry

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Effect of cadmium on early developmental stages of the sheepshead minnow (Cyprinodon variegatus)

Meteyer

Wright

Martin

1988

Enviro Toxic and Chemistry

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Cadmium uptake by the early developmental stages of the sheepshead minnow, Cyprinodon variegatus, was studied over a 7.5‐d post‐hatch period. After chorionic hardening (about 4 h after fertilization), eggs were placed in filtered water from the Patuxent River, Maryland (diluted to 5%90 salinity), and dosed with cadmium concentrations of between 0.39 and 1,020 μg L−1. At the highest cadmium concentrations studied, hatching was delayed up to 3 d and larvae had spinal deformities. Growth inhibition was not clearly dose‐related, although larvae from all cadmium treatments were significantly shorter than controls. The lowest dry weights were recorded from the 5.8 and 1,020 μg Cd L−1 treatment groups. Cadmium uptake peaked at 12 h after fertilization. Calcium levels in eggs and yolk‐sac larvae reared in 446 μg Cd L−1 were greater than in controls after the first 2 d of development but fell behind thereafter, reaching only 50% of the control calcium level after 7.5 d. Inhibition of calcium uptake persisted even after cadmium levels had peaked and fallen to the control level. Phosphorus and sodium concentrations in eggs and larvae were not affected by cadmium exposure. Ninety‐three percent of the cadmium accumulated by the egg was associated with the chorion, and 68% of this could be removed by a glycine buffer.

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The comparative influence of substituted phenols (especially chlorophenols) on yast cells assayed by elecro-rotation and other methods

Zimmermann

Pauli

et al. 1988

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Influence of nitrilotriacetic acid on Cd2+ uptake by yeast

Cited by 6 publications

References 14 publications

Cadmium‐uptake and influence on plasma membrane functions of yeast†

Cadmium‐uptake and influence on plasma membrane functions of yeast†

Effect of cadmium on early developmental stages of the sheepshead minnow (Cyprinodon variegatus)

The comparative influence of substituted phenols (especially chlorophenols) on yast cells assayed by elecro-rotation and other methods

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