Influence of nanoparticles on morphological differentiation of mouse embryonic stem cells

Tran, Diana; Ota, Lin C.; Jacobson, John D.; Patton, William C.; Chan, Philip J.

doi:10.1016/j.fertnstert.2006.07.1520

Cited by 23 publications

(16 citation statements)

References 50 publications

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“…This could have a bearing on tissue repair and maturation at the whole organism level, but could also influence embryogenesis where cell migration and compartmentalisation processes could be hindered leading to teratogenic effects in the absence of any apparent genetic damage or abnormality. In this context, Tran et al (2007) found plastic ENPs to produce developmental effects from within the cytoplasm by causing the reorganisation of the cytoskeleton in embryonic cells, therefore affecting cellular differentiation and morphology. Adhesion of mammalian endothelial cells has recently been shown to be significantly increased following exposure to alumina ENPs (Oesterling et al 2008), which supports the findings of the present study.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and cytotoxic potential of titanium dioxide (TiO2) nanoparticles on fish cells in vitro

2008

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Intrinsic genotoxic and cytotoxic potential of titanium dioxide (TiO2) engineered nanoparticles (ENPs) were evaluated in a metabolically competent, established fish cell line derived from rainbow trout (Oncorhyncus mykiss) gonadal tissue (i.e. RTG-2 cells). Prior to evaluation of the toxic potential, mean size of the ENPs was determined using transmission electron microscopy (TEM). As a prerequisite, an extensive characterisation of the ENPs was carried out following sonication which enabled the synthesis of an efficient dosing strategy for the cells in which exposure in phosphate buffered saline (PBS) gave an optimal agglomeration effects compared to distilled water (H2O) and minimal essential media (MEM). Interaction of the ENPs with cells under scanning electron microscope (SEM) was also studied. The genotoxic and cytotoxic potential of the ENPs were determined either alone or in combination with ultraviolet radiation (i.e. UVA). Whilst genotoxic potential was determined by evaluating DNA strand breaks using single cell gel electrophoresis (SCGE) or the comet assay and induction of cytogenetic damage using cytokinesis-blocked micronucleus (MN) assay, cytotoxicity was determined by measuring the retention of supra vital stain, neutral red, by the lysosomes using the neutral red retention (NRR) assay. In addition, while performing the comet assay, lesion specific bacterial endonuclease, formamidopyrimidine DNA glycosylase (Fpg), which recognises oxidised purine bases, was used to determine oxidative DNA damage. The results suggested that the highest concentration of the ENPs (i.e. 50 microg ml(-1)) did not produce elevations in DNA damage over 4 h (comet assay), 24 h (modified comet assay) or 48 h (MN assay) exposures in the absence of UVA irradiation, although there was a significant reduction in lysosomal integrity over 24 h exposure (NRR assay). The induction of MN did not show any enhanced levels as a function of ENP concentration. A significantly increased level of strand breaks was observed in combination with UVA (3 kJ m(-2)). In general, the NRR assay suggested elevated levels of cytotoxicity when the UVA exposure was carried out with MEM compared to PBS, although both showed an increase when in combination with the highest concentration of ENPs (i.e. 50 microg ml(-1)). Overall, the study emphasises the need for adoption of an holistic approach while evaluating the potential toxic effects of ENPs in which appropriate measures should be taken to avoid agglomeration or aggregation to facilitate efficient cellular uptake to evaluate potential biological responses.

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Section: Discussionmentioning

confidence: 99%

Genotoxic and cytotoxic potential of titanium dioxide (TiO2) nanoparticles on fish cells in vitro

2008

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“…272 273 It was reported that silica nanoparticles can inhibit differentiation to contractile myocardial cells in mouse EBs at concentrations at which cytotoxic effects were not observed in a test typically used to assess the embryo toxic potential of chemicals. [274][275][276][277][278][279][280] The potential for nanoparticle accumulation to transfer cumulatively to offspring should also be clearly addressed. 281 function over extended period of time; hence, the evaluation of potentially toxic effects should be performed immediately and for a long term as well.…”

Section: Applications In Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

Chen¹,

Qiu²,

Li³

2014

j biomed nanotechnol

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This paper reviews the recent studies on development of nanotechnology in the field of induced pluripotent and embryonic stem cells. Stem cell therapy is a promising therapy that can improve the quality of life for patients with refractory diseases. However, this option is limited by the scarcity of tissues, ethical problem, and tumorigenicity. Nanotechnology is another promising therapy that can be used to mimic the extracellular matrix, label the implanted cells, and also can be applied in the tissue engineering. In this review, we briefly introduce implementation of nanotechnology in induced pluripotent and embryonic stem cells research. Finally, the potential application of nanotechnology in tissue engineering and regenerative medicine is also discussed.

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“…A chimeric protein, GFP-FRATtide, attached to a hydrophobically modified 15-nm silica nanoparticle is efficiently delivered to the cytosol of human embryonic kidney cells and rat neural stem cells (Shah et al, 2011). Tran and colleagues studied the effects of nanoparticles on morphological differentiation in mES cells (Tran et al, 2007). However, so far, the efficiency of this technique is not high in ES cell studies.…”

Section: Usage Of Nanobeads As Carriers Of Functional Proteinsmentioning

confidence: 99%

Embryonic Stem Cells: Introducing Exogenous Regulators into Embryonic Stem Cells

Cheon¹

2011

Embryonic Stem Cells - Basic Biology to Bioengineering

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Influence of nanoparticles on morphological differentiation of mouse embryonic stem cells

Cited by 23 publications

References 50 publications

Genotoxic and cytotoxic potential of titanium dioxide (TiO2) nanoparticles on fish cells in vitro

Genotoxic and cytotoxic potential of titanium dioxide (TiO2) nanoparticles on fish cells in vitro

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

Embryonic Stem Cells: Introducing Exogenous Regulators into Embryonic Stem Cells

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