Influence of Monovalent Cations on Synthesis and Biological Activity of Nocardia vaccinii IMV B-7405 Surfactants

Пирог, Т. П.; Kliuchka, L.V.; Shevchuk, T.A.; Iutynska, G.O.

doi:10.15407/microbiolj81.04.015

Cited by 2 publications

(4 citation statements)

References 27 publications

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“…The results presented in this article are a continuation of our previous studies [5,6,11] on the possibility of regulating the antimicrobial and anti-adhesive activity of biosurfactants during the cultivation of a producer in a modified medium containing activators/inhibitors of enzymes responsible for the synthesis of components of the surfactants complex, which have certain biological properties. However, in our previous papers (unlike this article), the point at issue was increasing the antimicrobial and anti-adhesive activity of surfactants A. calcoaceticus ІМV B-7241, Rhodococcus erythropolis ІМV Ac-5017 and Nocardia vaccinii ІМV B-7405.…”

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesissupporting

confidence: 67%

“…Our studies [11] showed that potassium and sodium cations are activators of NADP +glutamate dehydrogenase in the Nocardia vaccinii ІМV B-7405 surfactant producer. Additional introduction of 0.5-1.0 g/l of sodium chloride, potassium chloride or a mixture of them in the cultivation medium of the strain ІМV B-7405 was accompanied by the synthesis of surfactants, the MIC of which relative to bacterial and yeast testing cultures were 2-125 and 2-30 fold lower, respectively, the adhesion of bacteria on abiotic materials treated with such surfactants is 1.1-1.6 fold lower, and the degree of destruction of yeast biofilms is 25-48 % higher compared to the indicators to established for surfactants obtained in the basal medium.…”

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 54%

“…The amount of extracellular surfactants was determined using our modified method of Bligh and Dyer after extraction with a mixture of chloroform and methanol (2:1) from the supernatant of the culture liquid as described in the study [11]. To obtain the supernatant, the culture liquid was centrifuged at 5000 g for 20 minutes.…”

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 99%

“…In paper [11], we summarized both our own experimental data on the regulation of the biological activity of biosurfactants, and some literature data on the effect of certain cations in nutrient media on the synthesis of both surfactants and other products of microbial synthesis.…”

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 99%

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Synthesis and Biological Activity of Acinetobacter calcoaceticus IMV B-7241 Surfactants Depending on Monovalent Cations Content in Cultivation Medium

Пирог¹,

Lutsai²,

Shevchuk³

et al. 2021

Mikrobiol. Z.

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Microbial surfactants (biosurfactants) are multifunctional preparations due to a combination of physicochemical (reduction of surface and interfacial tension, emulsifying activity) and biological (antimicrobial and antiadhesive activity, the ability to destroy biofilms) properties. However, the disadvantage of biosurfactants synthesized as a complex of compounds is the possibility of changing the biological activity depending on the conditions of producer cultivation. Aim. To study the effect of potassium and sodium cations on the NADP+-dependent glutamate dehydrogenase activity of cell-free extract of Acinetobacter calcoaceticus ІМV B-7241 with subsequent appropriate modification of the nutrient medium composition and determination of antimicrobial and anti-adhesive activity of surfactant synthesized. Methods. A. calcoaceticus ІМV B-7241 strain was grown in media containing 2% of sunflower oil waste as a carbon source, as well as various concentrations of potassium and sodium chloride (basal – 1.0 g/l NaCl, medium #1 that did not contains NaCl, medium #2 in which the concentration of NaCl was 2.0 g/l, medium #3 in which the concentration of NaCl and KCl was 1.0 g/l each). The surfactants were extracted from the supernatant liquid culture with a modified Folch mixture. Antiadhesive activity and the degree of biofilms degradation were determined by spectrophotometric method, antimicrobial activity − by the indicator of the minimum inhibitory concentration (MIC). Activity of enzymes of surface-active aminolipids biosynthesis (NADP+-dependent glutamate dehydrogenase) and glycolipids (phosphoenolpyruvate (PEP) carboxylase, PEP-synthetase, PEP-carboxykinase, trehalose phosphate synthase) were analyzed in cell-free extracts obtained after the destruction of cells by ultrasound. Results. It was found that potassium and sodium cations in concentrations of 50 and 100 mM are inhibitors of NADP+-dependent glutamate dehydrogenase, and in lower concentrations (5–20 mM) – activators of this enzyme, as well as PEP-carboxykinase and PEP-synthetase. The increase in the biosurfactant concentration to 6.1−7.7 g/l during cultivation of A. calcoaceticus ІМV B-7241 in medium #1 and #3 was due to the predominant synthesis of glycolipids under such conditions, which was evidenced by the increase in 1.8−6.5 times in the activity of PEP-carboxylase, PEP-carboxykinase, PEP-synthetase and trehalose phosphate synthetase compared to the indicators on the basal medium. The concentration of surfactants synthesized in the basal medium was 3.6 g/l, but such surfactants were characterized by the highest antimicrobial and anti-adhesive activity. Their MIC against the test-cultures of studied bacteria (Pseudomonas sp. MI-2, Bacillus subtilis BT-2, Escherichia coli IEM-1, Staphylococcus aureus BMS-1, Enterobacter cloaceae C-8) and fungi (Candida albicans D-6, Rhizopus nigricans P1, Aspergillus niger P-3, Fusarium culmorum T-7) were 0.88−56 μg/ml and were by 2−3 orders of magnitude lower compared to established for surfactants synthesized in modified media #1–3. In the case of treatment of abiotic materials with surfactant solutions obtained on the basal medium, the adhesion of bacteria and fungi was on average 10–20% lower than after surface treatment by the surfactant synthesized in modified media. In the presence of 148−296 μg/ml of surfactants obtained in the basal medium, destruction of S. aureus BMS-1 and B. subtilis BT-2 biofilms was 45−66%, and C. albicans D-6 yeast – 39−44%. Under the action of similar concentrations of surfactants synthesized in modified media, the destruction of bacterial and yeast biofilms was lower: 6-52 and 20–46%, respectively. Conclusions. The obtained results are consistent with the data of our previous studies on the possibility of regulating the antimicrobial and antiadhesive activity of surfactants in the process of producer cultivation by changing the content of cations in the medium, which are inhibitors/activators of enzymes responsible for the synthesis of components of the surfactants complex, which have certain biological properties.

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Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesissupporting

confidence: 67%

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 54%

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 99%

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 99%

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Synthesis and Biological Activity of Acinetobacter calcoaceticus IMV B-7241 Surfactants Depending on Monovalent Cations Content in Cultivation Medium

Пирог¹,

Lutsai²,

Shevchuk³

et al. 2021

Mikrobiol. Z.

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The Ways of Increasing Bioconversion of Crude Glycerol in Biosurfactants of Rhodococcus erythropolis ІМВ Аc-5017, Acinetobacter calcoaceticus ІМВ В-7241 and Nocardia vaccinii IМВ В-7405

Пирог¹,

Shevchuk²,

Mashchenko³

2015

Mikrobiol. Z.

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ПУТИ ПОВЫШЕНИЯ БИОКОНВЕРСИИ ТЕХНИЧЕСКОГО ГЛИЦЕРИНА В ПОВЕРХНОСТНО-АКТИВНЫЕ ВЕЩЕСТВА RHODOCOCCUS ERYTHROPOLIS IMВ Aс-5017, AСINETOBACTER CALCOACETICUS IMВ B-7241 И NOCARDIA VACCINII IMВ B-7405В предыдущих исследованиях [2, 3] нами была показана возможность синтеза поверхнос-тно-активных веществ при культивировании штаммов Rhodococcus erythropolis IMВ Ac-5017, Aсinetobacter calcoaceticus IMВ B-7241 и Nocardia vaccinii K-8 (IMВ B-7405) в среде с очи-щенным глицерином. Позже [4] при исследовании влияния компонентов технического глице-рина (соли калия и натрия, этанол, метанол) -побочного продукта производства биодизеля на образование ПАВ N. vacсinii K-8 было установлено, что внесение в среду с очищенным глицерином (1 %) хлорида калия (натрия) в концентрации 2,5 % и этанола (метанола) в кон-центрации 0,3 % сопровождалось повышением условной концентрации ПАВ в 1,4-1,7 раза по сравнению с показателями на среде без добавления солей и спиртов. Максимальная кон-центрация внеклеточных ПАВ (4,78 г/л) была достигнута при 4 % технического глицерина в среде культивирования N. vacсinii K-8, а дальнейшее увеличение содержания субстрата сопровождалось снижением показателей синтеза ПАВ. В работе [6] мы установили возмож-ность использования технического глицерина и для синтеза ПАВ штаммами IMВ Ac-5017 и IMВ B-7241, причем на отходах производства биодизеля концентрация ПАВ была в два раза выше, чем на эквимолярной по углероду концентрации очищенного субстрата. В этих иссле-дованиях содержание технического глицерина в среде не превышало 2 % (по объему) [6].В то же время, учитывая объемы производства биодизеля в мире -более 11 млн т в 2008 году с ежегодным последующим увеличением на 8−10 % [15], а также количество обра-зующегося в качестве отходов технического глицерина -10 % от получаемого биодизеля [13], становится понятно, что для эффективного использования такого отхода в качестве субстрата в биотехнологических процессах его содержание в среде культивирования продуцентов прак-тически ценных микробных метаболитов должно быть как можно выше.В святи с изложенным цель работы -установление условий культивирования штаммов R. erythropolis IMВ Ac-5017, A. calcoaceticus IMВ B-7241 и N. vaccinii IMВ B-7405 на среде с

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Influence of Monovalent Cations on Synthesis and Biological Activity of Nocardia vaccinii IMV B-7405 Surfactants

Cited by 2 publications

References 27 publications

Synthesis and Biological Activity of Acinetobacter calcoaceticus IMV B-7241 Surfactants Depending on Monovalent Cations Content in Cultivation Medium

Synthesis and Biological Activity of Acinetobacter calcoaceticus IMV B-7241 Surfactants Depending on Monovalent Cations Content in Cultivation Medium

The Ways of Increasing Bioconversion of Crude Glycerol in Biosurfactants of Rhodococcus erythropolis ІМВ Аc-5017, Acinetobacter calcoaceticus ІМВ В-7241 and Nocardia vaccinii IМВ В-7405

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