Influence of modified Tyrode's media on motility of cold-stored stallion spermatozoa

Webb, Gary W.; Arns, M.J.

doi:10.1016/s0737-0806(06)81836-8

Cited by 12 publications

(8 citation statements)

References 4 publications

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“…Sperm plasma membranes, however, suffered no obvious damage as the highest membrane integrity values were obtained in skim milk glucose extender. High sperm motility after storage in skim milk extender despite the removal of SP has also been reported (Webb and Arns, 1995). Our results suggest that sperm from different stallions differ in their sensitivity to the absence of SP in skim milk extender.…”

Section: Discussionsupporting

confidence: 80%

“…Before cooled semen storage, SP is either removed by centrifugation or its influence is reduced by dilution with an extender. Webb and Arns (1995) reported that the removal of SP and the dilution of sperm with skim milk extender containing modified Tyrode's medium (KMT) improved motion characteristics during cooled storage of spermatozoa. KMT, however, was detrimental to sperm motility in the presence of SP (Rigby et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Seminal Plasma Fractions on Stallion Sperm Survival after Cooled Storage

Akçay

Reilas

Andersson

et al. 2006

Journal of Veterinary Medicine Series A

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This study aimed to evaluate stallion sperm survival after 24 h of cooled storage in the presence of seminal plasma (SP) derived from the sperm-rich fractions (SRF) or sperm-poor fractions(SPF) of the ejaculate, without SP, or in the presence of SP from other stallions. Ejaculates were collected from four stallions using an automated phantom, which separated the semen into five cups. Centrifuged and washed spermatozoa from cup 2 (SRF) were mixed with skim milk extender to a concentration of 100 x 10(6) sperm/ml and then 1:1 (v/v) with SP from the stallion's own or another stallions' second (SP-SRF) or last cup (SP-SPF). Skim milk extender (K) and skim milk extender supplemented with modified Tyrode's medium (KMT) were used as control treatments. After a 24-h storage period in a transport container, spermatozoa were evaluated for motion characteristics and plasma membrane integrity by calcein acetoxymethyl (AM)/propidium iodide staining. The percentage of spermatozoa with intact plasma membranes after storage was lower in SP-SRF than in SP-SPF, and the highest in K (P < 0.05). Progressive motility (PMOT) was lower for sperm stored in SP-SRF than for sperm stored in SP-SPF (P < 0.05), but there was no significant difference in total motility (TMOT). Sperm stored in KMT (P < 0.05) registered the highest TMOT and PMOT percentages. Osmolarity was significantly higher and pH lower in K than in KMT or SP. Treatment with SP-SPF from three stallions benefited the PMOT of sperm from one stallion. These preliminary findings suggest that SP from SRFs may be more harmful during storage than SP from SPFs. Removal of SP improves sperm survival in KMT extender, and exchanging SP between stallions seems to influence sperm survival.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Effect of Seminal Plasma Fractions on Stallion Sperm Survival after Cooled Storage

Akçay

Reilas

Andersson

et al. 2006

Journal of Veterinary Medicine Series A

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“…Skim milk extenders may not be ideal if all seminal plasma has been removed (Jasko et al 1992). The negative effect of centrifugation in Expt 1 could perhaps have been altered by supplementing skim milk extender with a modified Tyrode's media (Padilla and Foote 1991;Webb and Arns 1995). Plasma membrane integrity was not significantly affected by the presence or absence of seminal plasma in our experiments, and there were no significant differences between cups.…”

Section: Discussionmentioning

confidence: 63%

“…1992). The negative effect of centrifugation in Expt 1 could perhaps have been altered by supplementing skim milk extender with a modified Tyrode's media (Padilla and Foote 1991; Webb and Arns 1995).…”

Section: Discussionmentioning

confidence: 99%

Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage

Kareskoski

Reilas

Andersson

et al. 2006

Reprod Domestic Animals

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With the aim of investigating properties of stallion seminal plasma to eventually improve semen-handling techniques, sperm motility and plasma membrane integrity were analysed in different fractions of the ejaculates after storage. Semen was collected using a computer-controlled automated phantom that separates the ejaculates into five successive cups. Samples containing seminal plasma and skim milk extender were compared with samples stored in skim milk extender after the removal of seminal plasma by centrifugation. Fractionated ejaculates were stored cooled for 24 h after dilution with extender (Expt 1) or frozen in liquid nitrogen (Expt 2). In Expt 1, cup 1 was pre-sperm fluid, cups 2 and 3 sperm-rich fractions, and cup 4 sperm-poor fractions. In Expt 2, cups 1 and 2 were sperm-rich fractions, and cups 3 and 4 sperm-poor fractions. One sample (WE) represented the whole ejaculate in both experiments. Motility parameters were determined with a Hamilton-Thorn Motility Analyzer, and plasma membrane integrity was assessed using carboxyfluorescein diacetate and propidium iodide staining and fluorescence microscopy. The removal of seminal plasma lowered motility values, but not plasma membrane integrity, in both experiments. No significant differences between cups were observed after cooled storage. The cups differed significantly in most post-thaw motility parameters, and the sperm-rich fraction showed higher post-thaw motility than the whole ejaculate.

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“…If all seminal plasma is removed, skim milk is not a suitable extender. Addition of a modified high-potassium Tyrode's medium to skim milk extender during cooled storage of ≥24 h in the total absence of seminal plasma has yielded good sperm motility; but if any seminal plasma is left, skim milk alone is better than addition of Tyrode's medium (Padilla and Foote 1991, Webb and Arns 1995, Rigby et al 2001. The effect of seminal plasma during storage may also depend on the temperature; 5°C, room temperature and 40°C have given different results (Wöckener et al 1990, Sieme et al 2001.…”

Section: Role Of Seminal Plasma In Semen Storagementioning

confidence: 99%

Components of stallion seminal plasma and their influence on spermatozoa

Katila¹,

Kareskoski²

2006

PHK

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SummaryThis review focuses on the composition of stallion seminal plasma and on its role as a storage medium of spermatozoa, with special reference to artificial insemination (AI). The stallion ejaculates in 6-9 jets resulting from urethral contractions, and these jets have characteristic volumes, sperm concentrations and composition. Different types of open-ended or closed artificial vaginas (AVs) are available for semen collection, and ejaculatory jets can be collected separately as a fractionated ejaculate, using an open-ended AV or an automated phantom (Equidame ® ). Seminal plasma influences spermatozoa during storage in different ways depending on dilution ratios, duration of storage and other factors. Beneficial effects have been reported after seminal plasma addition before freezing, but contradictory results show that short-term exposure (15 min) of sperm to seminal plasma has no effect on sperm motility or viability, and prolonged exposure (2, 4 or 6 h) to 5 or 20% seminal plasma in the freezing diluent is deleterious. The optimal proportion of seminal plasma for cooled storage seems to be fairly low. Figures from 0 to 20% have been reported in different studies. There is some evidence that the sperm-rich fractions of the ejaculate would be more suitable for freezing than the whole ejaculate or the sperm-low fractions. Seasonal and individual variation is seen in the composition and quality of stallion seminal plasma. Research on the biochemical composition of whole and fractionated stallion ejaculates, mainly concerning the levels of enzymes, carbohydrates, lipids, electrolytes and minerals, and compounds involved in the protection of sperm from oxidative damage is reviewed.Keywords: horse, reproduction, seminal plasma, spermatozoa, cryopreservation, cooled semen Zusammensetzung des Seminalplasmas beim Hengst und dessen Einfluss auf die SpermienDieser Übersichtsartikel befasst sich mit der Zusammensetzung des Seminalplasmas beim Hengst und dessen Bedeutung als Komponente in konserviertem Sperma unter besonderer Berücksichtigung der praktischen Anforderungen in der equinen Samenübertragung. Mithilfe urethraler Kontraktionen erfolgt die Ejakulation beim Hengst in 6-9 Fraktionen; diese Fraktionen unterscheiden sich hinsichtlich des Volumens, der Spermienkonzentration und der Zusammensetzung. Verschiedene Typen (geschlossene und offene Modelle) künstlicher Vaginen werden zur Samengewinnung eingesetzt. Die verschiedenen Ejakulatfraktionen können manuell -mit offenem Scheidenmodell -oder mithilfe eines automatisierten Phantoms (Equidame ® ) separat gewonnen werden. Seminalplasma beeinflusst die Spermien während der Lagerung in Abhängigkeit vom Verdünnungsgrad, der Lagerungsdauer und weiteren Faktoren. Positive Effekte auf die Spermaqualität wurden durch Seminalplasmazugabe vor der Tiefgefrierung des Samens erzielt; im Gegensatz dazu wird jedoch beschrieben, dass eine kurzzeitige Exposition (15 Min.) von Spermien mit Seminalplasma keine Effekte auf Spermienmotilität und -viabilität bedingt, eine prolongierte...

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Influence of modified Tyrode's media on motility of cold-stored stallion spermatozoa

Cited by 12 publications

References 4 publications

Effect of Seminal Plasma Fractions on Stallion Sperm Survival after Cooled Storage

Effect of Seminal Plasma Fractions on Stallion Sperm Survival after Cooled Storage

Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage

Components of stallion seminal plasma and their influence on spermatozoa

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