Influence of methyl derivatives of coumarin on mitotic activity and ultrastructure of meristematic cells of Allium cepa root tips

Kupidłowska, Ewa; Bieniak, B.; Ruchirawat, A.; Zobel, Alicja M.

doi:10.1016/s0944-7113(96)80055-9

Cited by 5 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monosubstituted simple coumarins, such as 6-methylcoumarin, 7-methylcoumarin, 7-methoxycoumarin as well as 5,7-dimethoxycoumarin, also induce mitotic aberrations analogous to those which were induced by scoparone (Nuti Ronchi & Arcara, 1967;Buiatti & Nuti Ronchi, 1969;Abel & Schimmer, 1981, Kupidĺowska et al, 1995. Moreover, the sister chromatid exchanges were also induced by Nmethylated oxypurines (Kihlman et al, 1971).…”

Section: Discussionmentioning

confidence: 99%

“…In our laboratory, the reaction of meristematic cells with several monosubstituted derivatives of coumarin was investigated independently of UV-A. The studies revealed that the intensity of mitodepression, its reversibility during postincubation, and disturbances of mitosis depend on the type and position of the substituent (Kupidĺowska et al, 1995;Podbielkowska et al, 1995). However, little is known about the effects exerted by disubstituted coumarin derivatives.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Effects of Two Disubstituted Coumarins on Mitosis and Respiration in Meristematic Cells

Kupidłowska

2001

Pharmaceutical Biology

View full text Add to dashboard Cite

Two disubstituted derivatives of coumarin (6,7-dihydroxycoumarin and 6,7-dimethoxycoumarin) were investigated for their effect on mitotic activity and energy metabolism of meristematic cells. Both coumarins lowered the rate of oxygen uptake by meristematic cells. It appeared that both compounds significantly reduced the number of cell divisions, although the methoxyl derivative caused a stronger mitostatic effect than the hydroxyl derivative. The mitostatic effect of the hydroxyl derivative was reduced whereas that of methoxyl derivative was enhanced by addition of exogenous ATP to the incubation medium. Both coumarins induced nonspecific disturbances of mitosis as well as specific chromosome aberrations. The number of the latter increased due to simultaneous treatment of cells with the tested compounds and ATP. The results obtained indicate that both compounds are antimitotic agents and that mitotic abnormalities are produced by different mechanisms, related or not related to the energy metabolism of the cell.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effects of Two Disubstituted Coumarins on Mitosis and Respiration in Meristematic Cells

Kupidłowska

2001

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…The absence of chromosomal aberrations has been observed when the mitodepressive activity of coumarin 200 ppm was investigated with Levan's test on meristematic cells from Allium cepa root tips (Podbielkowska et al, 1994). The probability that the energy metabolism of cells affected by coumarin is altered has been supported by observation of morphological changes in mitochondria, including dense matrices and large dilated cristae (Kupidlowska et al, 1996). Morphological alterations such as these lead to a loss of oxidative phosphorylation and the generation of ATP which is required for DNA synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…Coumarin (1,2-benzopyrone) is a higher plantderived secondary metabolite capable of altering the energy metabolism of cells by inhibiting glycolysis and oxidative phosphorylation (Kupidlowska et al, 1994(Kupidlowska et al, , 1996. Coumarin has been found to be a promising anticancer drug as it has exhibited antimitotic action on promeristematic cells of Allium cepa L. in Levan's test (Podbielkowska et al, 1994;Keightley et al, 1996) and lowered the vitality of melanoma cells without causing chromosomal aberrations (Wierzchowska-Renke et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Paclitaxel in Combination with Coumarin as a Potentially Effective Anticancer Agent

Zobel¹,

Schellenberger²

2000

Pharmaceutical Biology (Formerly International Journal of Pharm

Self Cite

View full text Add to dashboard Cite

show abstract

Aquatic Toxicity Assessment of the Additive 6-Methylcoumarine Using Four Experimental Systems

Jos

Repetto

Ríos

et al. 2008

Arch Environ Contam Toxicol

View full text Add to dashboard Cite

The toxicity assessment of chemicals is one of the main issues in the current policies in order to protect the health of the environment and human beings. Food and cosmetic additives have been extensively studied in relation to their toxicity to humans, but data about their ecotoxicological effects are scarce. The aim of this study was to evaluate the toxic effects of the additive 6-methylcoumarine in the aquatic milieu using a test battery comprising experimental model systems from different trophic levels. The inhibition of bioluminiscence was studied in the bacteria Vibrio fischeri (decomposer), the inhibition of growth was evaluated in the alga Chlorella vulgaris (producer) and immobilization was studied in the cladoceran Daphnia magna (first consumer). Finally, several end points were evaluated in the RTG-2 salmonid fish cell line, including neutral red uptake, protein content, methylthiazol tetrazolium salt metabolization, glucose-6-phosphate dehydrogenase activity, lactate dehydrogenase activity and leakage, and morphology. The sensitivity of the test systems employed was as follows: V. fischeri > D. magna > C. vulgaris > RTG-2 cell line. The results show that 6-methylcoumarine is not expected to produce acute toxic effects on the aquatic biota. However, chronic and synergistic effects with other chemicals cannot be excluded and should be further investigated.

show abstract

Influence of methyl derivatives of coumarin on mitotic activity and ultrastructure of meristematic cells of Allium cepa root tips

Cited by 5 publications

References 21 publications

The Effects of Two Disubstituted Coumarins on Mitosis and Respiration in Meristematic Cells

The Effects of Two Disubstituted Coumarins on Mitosis and Respiration in Meristematic Cells

Paclitaxel in Combination with Coumarin as a Potentially Effective Anticancer Agent

Aquatic Toxicity Assessment of the Additive 6-Methylcoumarine Using Four Experimental Systems

Contact Info

Product

Resources

About