Influence of Metalation on the Morphologies of Poly(ethylene oxide)-<i>b</i><i>lock</i>-poly(4-vinylpyridine) Block Copolymer Micelles

Sidorov, Stanislav N.; Bronstein, Lyudmila M.; Kabachii, Yuri A.; Valetsky, Pyotr M.; Soo, Patrick Lim; Maysinger, Dušica; Eisenberg, Adi

doi:10.1021/la0360658

Cited by 143 publications

(128 citation statements)

References 33 publications

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“…Upon micellization, the hydrophobic blocks interact with each other and form the core of the micelle while the hydrophilic blocks stay in contact with water to form the corona [3]. The shape and the size of the polymeric micelles are influenced by: 1) the copolymer chains size and chemical nature 2) The type of the solvent used to dissolve the copolymer and 3) the critical micelle concentrations of the copolymer [4,5,6]. Since polymeric micelles can solubilize poorly watersoluble drugs and have a long circulation time in blood as compared to other types of delivery systems, these vesicles are promising candidates for the next generation of drug carriers with targeted and controllable drug release [7].…”

Section: Introductionmentioning

confidence: 99%

Polymeric micelle-based bioassay with femtomolar sensitivity

Mouffouk

Chishti

Jin

et al. 2008

Analytical Biochemistry

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Target specific polymeric micelles loaded with fluorescence dye molecules in their hydrophobic cores were made from block co-polymer of poly(carprolactones) 23 -b-poly(ethylene oxide) 45 (PCL23-b-PEO45). It was found that the micelles are stable against pH changes from 2 to 12 and temperature variation up to 65 °C. The dye molecules can be released to the solution upon exposing the micelles to organic solvents or ultrasound. A rapid and highly sensitive immunoassay based on the above micelles was developed, and the assay can detect specific target proteins in femtomolar range from complex biological samples such as serum mimics and cell lysate. For example, less than 0.15 unit/ml of ovarian cancer specific antigen 125 (CA125), equivalent to 7.5 × 10 -15 M, can be reliably detected in solution. We also demonstrated the assay can detect a cell surface biomarker (SAAE-4) from a single human embryonic stem (hES) cell.

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Section: Introductionmentioning

confidence: 99%

Polymeric micelle-based bioassay with femtomolar sensitivity

Mouffouk

Chishti

Jin

et al. 2008

Analytical Biochemistry

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“…Pioneering research on pH and salt responsiveness involved synthetic polyelectrolytes such as poly(acrylic acid) and poly(vinyl pyridine) coupled to neutral, hydrophobic blocks (i.e., poly(butadiene)), where changes in pH or salt concentration impacted the ionic nature of the hydrophilic block. [36][37][38][39][40] Common examples of temperature responsiveness are a result of the incorporation of blocks that contain a lower critical solution temperature (LCST) such as poly(N-isopropyl acrylamide) 41 or poly(propylene oxide) 11 or the incorporation of polypeptides where the secondary structure transitions with temperature (i.e., a-helix to b-sheet). 42 Polypeptide-based block copolymers offer many advantages in the realm of responsive block copolymer self-assembly such as biocompatibility and enhanced biofunctionality.…”

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confidence: 99%

Self‐assembly and responsiveness of polypeptide‐based block copolymers: How “Smart” behavior and topological complexity yield unique assembly in aqueous media

Ray¹,

Johnson²,

Savin³

2013

J Polym Sci B Polym Phys

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Polypeptide-based amphiphilic block copolymers are an attractive class of materials given their ability to form welldefined aqueous nanoassemblies that respond to external stimulus through secondary structure transitions. This report will highlight recent literature in the area of polypeptide-based block copolymer self-assembly, with the major focus being on how the responsive nature and structural complexity of the polypeptide blocks can be incorporated into systems with complex topologies such as ABA/BAB/ABC triblock copolymers, AB 2 and A 2 B star copolymers, and miktoarm l-ABC star terpolymers. In particular, the role of interfacial curvature changes and how they result in morphology transitions will be discussed. The 'smart' assembly properties of peptides in complex block copolymer topologies can lead to enhanced responsiveness, morphological complexity, and unique morphological transitions with varying solution conditions. V C 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2013, 51, 508-523 KEYWORDS: amphiphiles; biomimetic; block copolymers; selfassembly INTRODUCTION Amphiphilic block copolymers are able to self-assemble into well-defined nanostructures in aqueous solution. [1][2][3][4][5] The equilibrium morphology and aggregation number of diblock copolymer assemblies is primarily determined by the balance of three energetic factors: (1) interfacial tension, (2) corona chain crowding, and (3) core chain stretching. The balance of these three factors dictates an equilibrium curvature for the aggregate. 6-8 Typically, solution morphologies formed by amphiphilic block copolymers follow a trend of increasing interfacial curvature. As the hydrophilic fraction is increased in the copolymer, vesicles, cylindrical micelles, and spherical micelles (in the order of increasing interfacial curvature,) are the most common morphologies (Figure 1). 5,9,10 Physically, this is explained as a balance between entropic freedom of the hydrophilic coronal chains and shielding of the hydrophobic blocks from the aqueous solution; as the hydrophilic fraction increases, the chains are more able to effectively stabilize these assemblies without close-packing, and the free energy of the system is lowered when the coronal chains are provided more entropic freedom/mobility through increased curvature.

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“…Gold nanoparticle containing micelles were prepared as described in detail (Sidorov et al 2004;Soo et al 2007). CdSe/ZnS, CdSe/ZnS/PEG and uncapped CdTe NPs were synthesized and characterized as per the method described previously (Gaponik et al 2002), and characterization was performed as described in our previous studies (Lovric et al 2005a ;Choi et al 2007).…”

Section: Nanoparticle Preparation and Characterizationmentioning

confidence: 99%

Mechanisms of cellular adaptation to quantum dots – the role of glutathione and transcription factor EB

Neibert

Maysinger

2011

Nanotoxicology

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Cellular adaptation is the dynamic response of a cell to adverse changes in its intra/extra cellular environment. The aims of this study were to investigate the role of: (i) the glutathione antioxidant system, and (ii) the transcription factor EB (TFEB), a newly revealed master regulator of lysosome biogenesis, in cellular adaptation to nanoparticle-induced oxidative stress. Intracellular concentrations of glutathione species and activation of TFEB were assessed in rat pheochromocytoma (PC12) cells following treatment with uncapped CdTe quantum dots (QDs), using biochemical, live cell fluorescence and immunocytochemical techniques. Exposure to toxic concentrations of QDs resulted in a significant enhancement of intracellular glutathione concentrations, redistribution of glutathione species and a progressive translocation and activation of TFEB. These changes were associated with an enlargement of the cellular lysosomal compartment. Together, these processes appear to have an adaptive character, and thereby participate in the adaptive cellular response to toxic nanoparticles.

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Influence of Metalation on the Morphologies of Poly(ethylene oxide)-block-poly(4-vinylpyridine) Block Copolymer Micelles

Cited by 143 publications

References 33 publications

Polymeric micelle-based bioassay with femtomolar sensitivity

Polymeric micelle-based bioassay with femtomolar sensitivity

Self‐assembly and responsiveness of polypeptide‐based block copolymers: How “Smart” behavior and topological complexity yield unique assembly in aqueous media

Mechanisms of cellular adaptation to quantum dots – the role of glutathione and transcription factor EB

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