Influence of major histocompatibility complex haplotype on the mitogenic response of T cells to staphylococcal enterotoxin B

Abstract

The abilities of antigen-presenting cells (APC) from nine independent major histocompatibility complex haplotypes and a number of intra-H-2 recombinant congenic strains of mice to present staphylococcal enterotoxin B (SEB) and induce proliferation in murine T-cell receptor Vi8' T-cell clones were compared. SEB presented by APC of all haplotypes tested induced significant responses in each of the T-cell clones. The magnitude of response was similar for most haplotypes, but there were limited quantitative differences between certain haplotypes. SEB presented by APC from H-2b mice as well as the intra-H-2 recombinant strains B1O.GD and B1O.A(4R), which do not express cell surface I-E (designated I-E-), induced the poorest T-cell responses. However, APC from AE-, A5E-, and AqE-mice were as potent in SEB presentation as APC expressing both I-A and I-E. Antibodies against I-E were more effective than anti-I-A antibodies at inhibiting responses to SEB presented by APC expressing both I-A and I-E, whereas responses induced by APC expressing I-A but not I-E were blocked by antibodies against I-A. Thus, our results show that I-A can present SEB efficiently but that expression of both I-A and I-E on the same APC results in presentation of SEB predominantly by I-E. In addition, experiments using four distinct I-E-strains of mice indicate that I-A alleles differ in their ability to present SEB.

Hock immunization: A humane alternative to mouse footpad injections

“…C57Bl/6 mice lack a functional MHC class II I-E (Jones et al, 1981;Mathis et al, 1983) which has been previously correlated with reduced immune responsiveness to certain proteins such as pigeon cytochrome c (PCC) (Schwartz et al, 1976;Solinger et al, 1979), Hen Egg Lysozyme (HEL) (Hill and Sercarz, 1975) and Hepatitis B antigen (HBsAg) (Neurath et al, 1983). Further, C57Bl/6 antigen presenting cells (APCs) were shown to be poor in inducing anti-staphylococcal enterotoxin B (SEB) responses from SEB-specific T cell clones (Robinson et al, 1991) suggesting that it is possible that fewer peptides from OVA and hALAC are presented in C57Bl/ 6 than in other strains thereby generating a lower magnitude of response. One reason for the recent increase in popularity of C57Bl/6 mice in immunological models in spite of its relatively poor CD4 response is the explosion in gene targeting studies employing the "knock out" technology.…”

Induction of tumor necrosis factor and interleukin-1 by purified staphylococcal toxic shock syndrome toxin 1 requires the presence of both monocytes and T lymphocytes

“…on July 31, 2020 by guest http://iai.asm.org/ Downloaded from T-cell clone is dominated by I-E (26). However, in the absence of I-E, some antigen-presenting cells bearing I-A alleles can present SEB as effectively as those expressing both I-A and I-E.…”

Hierarchy of Spea Presentation to T Cells by Mouse MHC Haplotypes

“…A previous study of the presentation of rSEB by mouse APC suggested that alleles of MHC class II molecules differ in their ability to present SEB to mouse T-cell clones (Robinson et al, 1991) and that rSEB is presented predominantly by E molecules, although A molecules are able to present in the absence of E. The results obtained with SPEA show significant differences when compared to those obtained with SEB, suggesting that the differences in sequence at the putative MHC binding site cause different binding affinities for MHC class II. The presentation of SEB by E molecules, overall, was relatively uniform, while SPEA binding was more variable.…”

“…The points on each line correspond to rSPEA concentrations 1 × 10 -7 , 5 × 10 -8 , 1 × 10 -8 , 5 × 10 -9 , 1 × 10 -9 and 0 M from left to right. MHC class II molecules in normal mice (Sant & Germain, 1989) and T-cell responses have been reported which are restricted by Aα d Eβ d molecules (Ruberti et al, 1992). Thus, it is possible that SPEA is presented by Aα s Eβ s but not by Aα b Eβ b dimers.…”