Influence of lignin and its degradation products on enzymatic hydrolysis of xylan

Modifying solubility of polymeric xylan extracted from Eucalyptus grandis and sugarcane bagasse by suitable side chain removing enzymes

“…4). Lower lignin contents may benefit the enzymatic modification by reducing lignin linkages to arabinose and MeGlcA residues, and thereby reduce the associated steric hindrances to enzyme access (Kaya, Heitmann, & Joyce, 2000;Wallace, Chesson, Lomax, & Jarvis, 1991).…”

Bioethanol production from Lantana camara (red sage): Pretreatment, saccharification and fermentation

“…Since a significant negative correlation exists between the percentage of lignin in plant material and its enzymatic digestibility (Kaya et al, 2000), in order to improve the enzymatic hydrolysis of L. camara, the lignin removal is essential. The chemical delignification of acid treated L. camara displayed a continuous increase in phenolics with the increase in sodium sulphite from 5.0% to 20.0% (w/v) ( Table 2).…”

“…The cellulase components such as b-glucosidase and endoglucanase showed higher binding affinity towards lignin compared to carbohydrates, which in turn lowered the saccharification efficiency (Kaya et al, 2000). Various delignification approaches have been exploited in the past such as alkali pretreatment (Carillo et al, 2005), hydrogen peroxide pretreatment (Saha and Cotta, 2007), sulphite pretreatment (Kuhad et al, 1999), ammonia fiber expansion pretreatment (Teymouri et al, 2005) and sodium chlorite pretreatment (Gupta et al, 2009).…”

The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain

“…Furthermore, the efficient use of enzymes depends on process variables such as temperature, pH, reaction time, enzyme concentration, pulp consistency (substrate concentration), intensity of agitation, and presence of other chemical species that may inhibit or accelerate their rates of reaction (Kaya et al 2000). Consequently, for the enzymatic process be performed with high efficiency, an optimization of the hydrolysis conditions is required.…”