Bioethanol production from Lantana camara (red sage): Pretreatment, saccharification and fermentation

Kuhad, Ramesh Chander; Gupta, Rishi; Khasa, Yogender Pal; Singh, Ajay

doi:10.1016/j.biortech.2010.06.043

Cited by 167 publications

(83 citation statements)

References 35 publications

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“…The decrease of ethanol concentration might be related to the conversion of ethanol to acetic acid. It has been reported that the microbial population was adapted to consume sugar and ethanol when the ethanol was accumulated in the medium thus resulted in reduction of ethanol concentration [13]. Similar result of decline in ethanol production after maximum ethanol production was reported in other study [14].…”

Section: Ssf and Shfsupporting

confidence: 82%

Ethanol Production from Seaweed, Enteromorpha intestinalis, by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) with Saccharomyces cerevisiae

Cho¹,

Kim²,

Kim³

2013

KSBB Journal

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Ethanol productions were performed by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes using seaweed, Enteromorpha intestinalis (sea lettuce). Pretreatment conditions were optimized by the performing thermal acid hydrolysis and enzymatic hydrolysis for the increase of ethanol yield. The pretreatment by thermal acid hydrolysis was carried out with different sulfuric acid concentrations in the range of 25 mM to 75 mM H 2 SO 4 , pretreatment time from 30 to 90 minutes and solid contents of seaweed powder in the range of 101 6% (w/v). Optimal pretreatment conditions were determined as 75 mM H 2 SO 4 and 13% (w/v) slurry at 121 o C for 60 min. For the further saccharification, enzymatic hydrolysis was performed by the addition of commercial enzymes, Celluclast 1.5 L and Viscozyme L, after the neutralization. A maximum reducing sugar concentration of 40.4 g/L was obtained with 73% of theoretical yield from total carbohydrate. The ethanol concentration of 8.6 g/L of SHF process and 7.6 g/L of SSF process were obtained by the yeast, Saccharomyces cerevisiae KCTC 1126, with the inoculation cell density of 0.2 g dcw/L.

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Section: Ssf and Shfsupporting

confidence: 82%

Ethanol Production from Seaweed, Enteromorpha intestinalis, by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) with Saccharomyces cerevisiae

Cho¹,

Kim²,

Kim³

2013

KSBB Journal

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“…The results (Table 2) indicate that an increase in the concentration of NaOH causes an increase in the biomass loss of 37%, 49%, and 52%, respectively, for catalyst concentrations of 1%, 6%, and 11%. A similar dependence was obtained in [12,37,38]. The research results indicate that the use of 1% NaOH already significantly lowers the lignin content.…”

Section: Influence Of Alkaline Pre-treatment Conditions Of the Chemicsupporting

confidence: 81%

Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation

et al. 2018

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This paper concerns the comparison of the efficiency of two-stage hydrolysis processes, i.e., alkaline pre-treatment and acid hydrolysis, as well as alkaline pre-treatment followed by enzymatic hydrolysis, carried out in order to obtain reducing sugars from triticale straw. For each of the analyzed systems, the optimization of the processing conditions was carried out with respect to the glucose yield. For the alkaline pre-treatment, an optimal catalyst concentration was selected for constant values of temperature and pre-treatment time. For enzymatic hydrolysis, optimal process time and concentration of the enzyme preparation were determined. For the acidic hydrolysis, performed with 85% phosphoric acid, the optimum temperature and hydrolysis time were determined. In the hydrolysates obtained after the two-stage treatment, the concentration of reducing sugars was determined using HPLC. The obtained hydrolysates were subjected to ethanol fermentation. The concentrations of fermentation inhibitors are given and their effects on the alcoholic fermentation efficiency are discussed.

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“…The primary sludge was freeze dried with a Vis Tri Freezemobile 35 Freeze Dryer (SP Industries Inc., Warminster, PA) at -80 °C. Since the fresh materials had a high moisture content (above 65%), the WS and freeze-dried PS were mechanically cut into small pieces by a chopper, as described by other investigators (Kuhad et al 2010;Gupta et al 2011), and stored in sealed plastic bags at room temperature. The moisture content for each substrate was adjusted to 62 ± 2% (v/w) prior to use.…”

Section: Raw Materials and Biomass Preparationmentioning

confidence: 99%

“…For the last two decades, among various industrial enzymes, cellulases, xylanases, and laccases have gained an enormous amount of attention for their potential applications in the bioconversion of biomass and other biotechnological applications. These enzymes have been used in such applications as bio-stoning and bio-polishing of jeans, improving efficacy of detergents, retting of flax, bio-pulping, treatment of wastewater polluted by dyes and other organic pollutants, development of biosensors, improving nutritional properties of animal feed, maceration and color extraction from juices, and the production of oligosaccharides (Xu 2005;Kuhad and Singh 2007;Kuhad et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Potential Application of Ganoderma lucidum in Solid State Fermentation of Primary Sludge and Wheat Straw

Jesus¹,

Sain²,

Jeng³

et al. 2015

BioResources

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aThis study was conducted to investigate the production of lignocellulolytic enzymes and sugars by the fungus Ganoderma lucidum during solid state fermentation (SSF) using primary sludge (PS) and wheat straw (WS) as substrates at different concentration ratios. For fungal growth on SSF, 20 g of each blended substrate was added to Erlenmeyer flasks, which were autoclaved and maintained at room temperature prior to inoculation, whereas for submerged fermentation (SF), flasks containing 25 mL of potato dextrose broth (PDB) were used as standard to check the differences between both methods of growth, and then all flasks were incubated at 25 °C in the dark, during 8 and 16 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis from the protein extract obtained from solid state fermentation strongly suggested that G. lucidum could produce lignocellulolytic enzymes to degrade primary sludge and wheat straw. Among the sugars, the production of xylose and mannose was disturbed by adding primary sludge. With the addition of primary sludge, high glucuronic acid content was observed. The results suggest that the combination of primary sludge and wheat straw, at concentration ratios of 1:1 to 1:3, respectively, can be used as a raw material in the production of lignocellulolytic enzymes and the bioconversion of other types of biomass by G. lucidum.

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Bioethanol production from Lantana camara (red sage): Pretreatment, saccharification and fermentation

Cited by 167 publications

References 35 publications

Ethanol Production from Seaweed, Enteromorpha intestinalis, by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) with Saccharomyces cerevisiae

Ethanol Production from Seaweed, Enteromorpha intestinalis, by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) with Saccharomyces cerevisiae

Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation

Potential Application of Ganoderma lucidum in Solid State Fermentation of Primary Sludge and Wheat Straw

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