Every month, about 40 tons of waste prunings of trees and grasses are wasted in Bauru, São Paulo, Brazil, occupying unimaginable volumes in the municipal landfill. This rich material has been tested for cultivation of medicinal mushroom Ganoderma lucidum, rather unknown in Brazil, compared to commonly use eucalyptus sawdust. Thus, the objective of this study was to evaluate the use of wasted urban vegetable pruning for the production of the G. lucidum mushroom. For so, these wastes were collected and tested with five different substrates with mixtures of tree pruning and pruning of grasses, changing the percentage content, plus a control of eucalyptus sawdust base (T) in two strains of G. lucidum, a total of 12 experimental treatments (substrates × strains) with 7 replicates each totaling 84 packages with 600 g the unit. The experimental cultivation was from August to December with 21 days of incubation over 67 days of production. Through the statistics of the Carbon/Nitrogen relation, biological efficiency, loss of organic matter and mass of fresh and dried mushroom, only the control treatment maintained a good performance. Pruning substrates trees and grasses have varied low to medium fungal biomass conversion potential.