Influence of ionic parameters on the growth and distribution of Boekelovia hooglandii (Chromophyta)

Barclay, William R.; Johansen, Jeffrey R.; Terry, Kenneth L.; Toon, S.

doi:10.2216/i0031-8884-30-4-355.1

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…More recently, Toon (15) suggested an alternative taxonomic placement in the Prymnesiophyceae. Further support for this taxonomic revision has been obtained from a comparison of fatty acid analysis of B. hooglandii with those of known prymnesiophytes (2,16,17). since the initial observation by Nicolai and Baas Becking (13), there have been no further studies of B. hooglandii until recent reports by Toon (15) and by Barclay et al (2) on a North American strain of this alga, which was found to grow well in media with high monovalent to divalent cation ratios.…”

Section: Introductionmentioning

confidence: 90%

Growth and osmoregulation of Boekelovia hooglandii in relation to salinity

Fujii¹,

Hellebust²

1994

Can. J. Bot.

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FuJn, S., and HELLEBUST, J. A. 1994. Growth and osmoregulation of Boekelovia hooglandii in relation to salinity. Can. J. Bot. 72: 823-828. t The growth and osmoregulation of Boekelovia hooglandii Nicolai et Baas Becking (Chrysophyceae) was investigated as a function of salinity. This chromophyte microflagellate was found to be euryhaline and also to require sodium for growth. The optimum sodium chloride concentration for growth was 0.2-0.4 M, and growth was severely inhibited in media with sodium chloride concentratioris above 1 M. Using gas chromatography -mass spectroscopy analysis of cell extracts, it was established that the alga contains D-mannitol, myo-inositol, as well as the inositol derivative, cyclohexanetetrol. The content of cyclohexanetetrol and mannitol increased with increased salinity, while the content of inositol remained almost constant. Potassium and magnesium were the major intracellular cations. However, the content of cations and amino acids showed only minor increases with salinity over a wide salinity range. The contents of cyclohexanetetrol and mannitol increased rapidly when cells were subjected to hypertonic shocks. It was concluded that B. hooglandii utilizes cyclohexanetetrol and mannitol as osmoregulatory substances. FUJH, S., et HELLEBUST, J.A. 1994. Growth and osmoregulation of Boekelovia hooglandii in relation to salinity. Can. J. Bot.72 : 823-828. Les auteurs ont CtudiC la croissance et 1'osmorCgulation chez le Boekelovia hooglandii Nicolai et Baas Becking (Chrysophyceae) en relation avec la salinitC. Cette microflagellte chromophyte s'est avCrCe euryhaline et a besoin de sodium pour sa croissance. La teneur optimum en chlorure de sodium pour la croissance est de 0,2-0,4 M, et la croissance est fortement inhibCe dans les milieux oh la teneur en chlorure de sodium dkpasse 1 M. Des analyses par chromatographie gazeuse couplCes avec la spectroscopie de masse conduites sur des extraits cellulaires montrent que l'algue contient du D-mannitol, du myoinositol ainsi qu'un dCrivC de l'inositol, le cyclohexanetCtro1. Les teneurs en cyclohexanetCtrol et en mannitol augmentent avec une augmentation de la salinitk, alors que la teneur en inositol demeure i peu p r b constante. Cependant, la teneur en cations et en acides aminCs ne montrent que de faibles augmentations avec la salinitC i 1'intCrieur d'un large Cventail de salinite. Les teneurs en cyclohexanetCtro1 et-en mannitol augmentent rapidement lorsque les cellules sont soumises 2 des chocs hypertoniques. Les auteurs concluent que le B. hooglandii utilise le cyclohexanetCtro1 et le mannitol comme substances osmorkgulatrices.

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Section: Introductionmentioning

confidence: 90%

Growth and osmoregulation of Boekelovia hooglandii in relation to salinity

Fujii¹,

Hellebust²

1994

Can. J. Bot.

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“…Boekelovia hooglandii Nicolai et Baas Becking is a unicellular microalga belonging to Chromophyta (Toon 1987;Volkman et al 1989) and occurs most abundantly in saline environments characterized by high ratios of monovalent to divalent cations (Barklay et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Effects of sodium chloride on the fatty acids composition in Boekelovia hooglandii (Ochromonadales, Chrysophyceae)

et al. 2001

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SUMMARY Composition of fatty acids in Boekelovia hooglandii Nicolai et Baas Becking (Chrysophyceae) was investigated as a function of salinity. It was confirmed by gas chromatography that the composition of fatty acids in cells cultured in a 50 mmol L−1 NaCl medium consisted of C14:0, C15:0, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C18:4, C20:0, C20:4, C20:5, C22:5 and C22:6, in which C14:0, C16:0, C16:1, C18:4, C20:0, C20:5, C22:5 and C22:6 were main constituents. When the cells were cultured in a medium with different concentrations of NaCl ranging from 50 to 800 mmol L−1, the mole percentage of fatty acids such as C14:0, C16:0 and C16:1 decreased with increases in the salinity, while the mole percentage of highly polyunsaturated fatty acids such as C18:4, C20:5, C22:5 and C22:6 increased. When the cells were transferred from a 200 mmol L−1 NaCl medium to a 600 mmol L−1 NaCl medium, a decrease in mole percentage of C14:0, C16:0 and C16:1, and an increase in C18:4, C20:5, C22:5 and C22:6 were observed within 4 h. However, no change in the compositions of fatty acids was observed within 4 h when the cells were transferred from a 600 mmol L−1 NaCl medium to a 200 mmol L−1 NaCl one. The increase in the content of highly polyunsaturated fatty acids was considered to reflect the rapid response to upshock and to be the characteristic of salt tolerance in B. hooglandii.

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