Influence of Interferons on the Repair of UV-Damaged DNA

Tsoncheva, Vania L.; Todorovа, Katerina; Ivanov, Iván; Maximova, Vera

doi:10.1515/znc-2008-3-423

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…It was shown, that both types of interferon (IFN-α and IFN-γ) have an inhibiting effect on DNA repair in both cell lines tested. DNA repair suppression by IFN-α was much more pronounced than that caused by IFN-γ (Tsoncheva et al, 2008). In summary, using two different test systems, we have now established that human interferons possess a strong inhibitory effect on repair of UVinduced damage of DNA.…”

Section: Resultsmentioning

confidence: 77%

Decreased DNA Repair Capacity of UV-Irradiated Cells Following Interferon Treatment

Tsoncheva

Todorovа²,

Maximova

2008

Zeitschrift Für Naturforschung C

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The aim of this study was to examine the effect of interferons (IFNs) on the recovery of UV-damaged cells by means of measuring cell viability rates. The influence of the recombinant human interferons IFN-α, IFN-and IFN-γ on the repair capacity of the UV-irradiated human cell lines WISH and HeLa was studied. The ability of cells to repair UV-induced damage was determined by the comet assay and both short-and long-term survival assays in proliferating cell cultures. We found that INFs negatively regulated DNA repair in cells damaged by UV light. One day after treatment, in both cell lines tested, IFN-α had a stronger inhibitory effect than IFN-γ. Combined treatment with different IFNs exhibited a stronger inhibitory effect on cell recovery than treatment with each of them. The protein kinase inhibitor wortmanin further aggravated the effect of IFNs on cell survival.

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Section: Resultsmentioning

confidence: 77%

Decreased DNA Repair Capacity of UV-Irradiated Cells Following Interferon Treatment

Tsoncheva

Todorovа²,

Maximova

2008

Zeitschrift Für Naturforschung C

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“…The nonsignificant change compared to the positive control in the level of micronuclei in bone marrow cells when using both gentabiferon-B and biferon-B where cytogenetic instability was induced by MMC indicates that there are no antimutagenic properties of gentabiferon-B. The presented data may be explained by the ability of interferons to exhibit antimutagenic activity, such as DNA repair, depending on the type of interferon and the genotype of the cell (13,25).…”

Section: Discussionmentioning

confidence: 89%

Study of Mutagenic and Antitoxic Properties of Gentabiferon-B

Shabunin

Gritsyuk

Востроилова

et al. 2022

Macedonian Veterinary Review

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The combination of immunomodulators and antibiotics in the treatment of animals with diseases of bacterial etiology is one of the effective strategies for animal therapy. The drug gentabiferon-B combines antibiotic gentamicin and species-specific (bovine) recombinant interferons -α and -γ. The study aimed to evaluate the effect of course application of gentabiferon-B on the cytogenetic stability of bone marrow cells of outbred mice after administering mitomycin C (MMC). The proportion of polychromatophilic erythrocytes in the bone marrow was assessed. There was no effect of gentabiferon-B on the frequency of polychromatophilic erythrocytes with micronuclei in both healthy animals and mice with MMC-induced cytogenetic instability. The course application of gentabiferon-B before the administration of MMC led to an increase in the proportion of polychromatophilic erythrocytes (46.03±2.61%) which was non-significantly different than the negative control group. The administration of MMC alone caused a decrease in the proportion of polychromatophilic erythrocytes to 33.33±1.83%. The antitoxic effect of gentabiferon-B led to an increase in the level of polychromatophilic erythrocytes by 38.1% compared to the group that received only MMC. Studies have shown that gentabiferon-B does not have mutagenic activity and anticlastogenic properties, however, it reduces the toxic effect of MMC. In conclusion, it is indicative that gentabiferon-B has antitoxic properties and can be safely used in animal therapy.

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“…Overall, this indicates several abnormalities in the DRC of pre-aHCT patient samples, which raises the question of the ability of such samples to reliably represent an individual’s repair capacity. It has been suggested that DRC in lymphocytes of cancer patients might be greatly affected by the general inflammation associated with the disease [ 57 – 59 ] or possibly by the cancer treatment itself. Consistent with that hypothesis, we had observed that DSB repair measurements by host-cell reactivation assays, even in lymphocytes from healthy individuals, could be influenced by factors in the environment of the cells, such as the presence of reactive oxygen species [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Autologous hematopoietic stem cell transplantation in lymphoma patients is associated with a decrease in the double strand break repair capacity of peripheral blood lymphocytes

Lacoste¹,

Bhatia

Chen

et al. 2017

PLoS ONE

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Patients who undergo autologous hematopoietic stem cell transplantation (aHCT) for treatment of a relapsed or refractory lymphoma are at risk of developing therapy related- myelodysplasia/acute myeloid leukemia (t-MDS/AML). Part of the risk likely resides in inherent interindividual differences in their DNA repair capacity (DRC), which is thought to influence the effect chemotherapeutic treatments have on the patient’s stem cells prior to aHCT. Measuring DRC involves identifying small differences in repair proficiency among individuals. Initially, we investigated the cell model in healthy individuals (primary lymphocytes and/or lymphoblastoid cell lines) that would be appropriate to measure genetically determined DRC using host-cell reactivation assays. We present evidence that interindividual differences in DRC double-strand break repair (by non-homologous end-joining [NHEJ] or single-strand annealing [SSA]) are better preserved in non-induced primary lymphocytes. In contrast, lymphocytes induced to proliferate are required to assay base excision (BER) or nucleotide excision repair (NER). We established that both NHEJ and SSA DRCs in lymphocytes of healthy individuals were inversely correlated with the age of the donor, indicating that DSB repair in lymphocytes is likely not a constant feature but rather something that decreases with age (~0.37% NHEJ DRC/year). To investigate the predictive value of pre-aHCT DRC on outcome in patients, we then applied the optimized assays to the analysis of primary lymphocytes from lymphoma patients and found that individuals who later developed t-MDS/AML (cases) were indistinguishable in their DRC from controls who never developed t-MDS/AML. However, when DRC was investigated shortly after aHCT in the same individuals (21.6 months later on average), aHCT patients (both cases and controls) showed a significant decrease in DSB repair measurements. The average decrease of 6.9% in NHEJ DRC observed among aHCT patients was much higher than the 0.65% predicted for such a short time frame, based on ageing results for healthy individuals.

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Influence of Interferons on the Repair of UV-Damaged DNA

Cited by 4 publications

References 23 publications

Decreased DNA Repair Capacity of UV-Irradiated Cells Following Interferon Treatment

Decreased DNA Repair Capacity of UV-Irradiated Cells Following Interferon Treatment

Study of Mutagenic and Antitoxic Properties of Gentabiferon-B

Autologous hematopoietic stem cell transplantation in lymphoma patients is associated with a decrease in the double strand break repair capacity of peripheral blood lymphocytes

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