Influence of In Vitro Human Digestion Simulation on the Phenolics Contents and Biological Activities of the Aqueous Extracts from Turkish Cistus Species

İnan, Yiğit; Akyüz, Selin; Kurt-Celep, İnci; Celep, Engin; Yeşilada, Erdem

doi:10.3390/molecules26175322

Cited by 8 publications

(6 citation statements)

References 53 publications

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“…The α-glucosidase inhibitory effect of C. parviflorus and other species of Cistus growing in Turkey has been reported and revealed the potential antidiabetic effect of the Cistus species through their ability to inhibit the diabetic-related enzymes, e.g., α-glucosidase, α-amylase, and advanced-glycation end products . The current finding of the α-glucosidase activity of the plant can be attributed to the presence of phenolic acids and flavonoids in the plant extract, as have been reported for such classes of natural products. , …”

Section: Resultsmentioning

confidence: 73%

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Polyphenol Fingerprint, Biological Activities, and In Silico Studies of the Medicinal Plant Cistus parviflorus L. Extract

El-Shibani,

Sulaiman,

Abouzied

et al. 2023

ACS Omega

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Cistus parviflorus L. (Cistaceae) is a medicinal plant with several folkloric applications, including being used for urinary tract infections and as a food additive. In this study, the polyphenolic diversity and the antioxidant, antidiabetic, and antimicrobial activities of the C. parviflorus methanolic extract were evaluated. Spectrophotometric and HPLC-based analyses using standard polyphenolic compounds were conducted to measure the phenolics and flavonoids in the plant extract. The in vitro DPPH, ORAC, FRAP, and α-glucosidase assays were used to evaluate the plant’s antioxidant and antidiabetic activities. Furthermore, disc diffusion and MIC-based microdilution tests were applied to evaluate the antimicrobial activity of the plant against broad-spectrum microorganisms. The analysis revealed the existence of high phenolic and flavonoid quantities that were measured at 302.59 ± 0.6 μg GAE and 134.3 ± 0.5 μg RE, respectively. The HPLC-based analysis revealed the existence of 18 phenolic acids and 8 flavonoids. The major phenolic acid was ellagic acid (169.03 ppm), while catechin was the major flavonoid (91.80 ppm). Remarkable antioxidant activity was measured using three different assays: DPPH, ORAC, and FRAP. Furthermore, strong inhibition of α-glucosidase compared to acarbose was recorded for the plant extract (IC 50 0.924 ± 0.6). The results showed that C. parviflorus ’s extract had a strong anti- Escherichia coli effect with MIC value of 0.98 μg\mL and IZD value of 32.2 ± 0.58 mm compared to 25.3 ± 0.18 mm for gentamycin, the positive control. Moreover, Aspergillus niger , Aspergillus fumigatus , Staphylococcus aureus , Streptococcus pyogenes , and Salmonella typhimurium all showed significant growth inhibition in response to the extract, a result that may be related to the use of the plant in traditional medicine to treat urinary tract infections. The docking study indicated the higher binding affinity of the major identified compounds, i.e., ellagic acid, rutin, naringin, catechin, and punicalagin, to the S. aureus gyrase-DNA complex, which might suggest the possible mechanisms of the plant as antimicrobial agents.

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Section: Resultsmentioning

confidence: 73%

“… 40 The current results are also comparatively similar to the reported results for C. parviflorus and other species of Cistus growing in Turkey. 51 …”

Section: Resultsmentioning

confidence: 99%

Polyphenol Fingerprint, Biological Activities, and In Silico Studies of the Medicinal Plant Cistus parviflorus L. Extract

El-Shibani,

Sulaiman,

Abouzied

et al. 2023

ACS Omega

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“…Distilled water was used to prepare the working concentration of P. auriculata . The experimental procedure was reported in detail in our previous studies, and different concentrations of quercetin were used as a reference material [ 33 , 36 , 37 , 38 , 39 ].…”

Section: Methodsmentioning

confidence: 99%

“…Thus, 32 was related to 7,4 -dihydroxyflavone O-hexoside-O-ethylmaleate (Table 2). In addition, seven diglycosides (33)(34)(35)(36)(37)(38)(39) and four triglycosides (40)(41)(42)(43) were tentatively identified in the studied Primula extracts (Table 2). Compounds 6,9,11,12,13,16,17,22,24,26,30,31,33,35,37,and 38 were identified by comparison with authentic standards.…”

Section: Flavones and Flavonolsmentioning

confidence: 98%

“…The reference standards used for compound identification were obtained as follows: protocatechuic acid (1), 4 -hydroxyflavone (6), apigenin (9), luteolin (11), kaempferol (12), quercetin (13), rhamnetin (16), isorhamnetin (17), apigenin 7-O-glucoside (22), luteolin 7-O-glucoside (24), kaempferol 3-O-glucoside (26), myricitrin (30), isoquercitrin (31), acaciin (33), kaempferol 3-O-rutinoside (35), rutin (37), and pectolinarin (38) were obtained from Extrasynthese (Genay, France); caffeic acid (2) and neochlorogenic acid (3) were supplied by Phytolab (Vestenbergsgreuth, Germany); and chlorogenic acid (4) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile (hypergrade for LC-MS), formic acid (HPLC-grade), and methanol (analytical-grade) were purchased from Merck (Darmstadt, Germany).…”

Section: Chemicalsmentioning

confidence: 99%

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An In-Depth Study on the Metabolite Profile and Biological Properties of Primula auriculata Extracts: A Fascinating Sparkle on the Way from Nature to Functional Applications

et al. 2022

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The biological activity of the aerial part and rhizomes of Primula auriculata were assessed for the first time. The biological activities (antioxidant properties, enzyme inhibition, and AGE inhibition) as well as the phenolic and flavonoid contents of the ethyl acetate, ethanol, hydro-ethanol and water extracts of P. auriculata aerial parts and rhizomes were determined. Cell viability assays and gelatin zymography were also performed for MMP-2/-9 to determine the molecular mechanisms of action. The gene expression for MMPs was described with RT-PCR. The levels of various proteins, including phospho-Nf-κB, BCL-2, BAX, p-53, and cyclin D1 as well as RAGE were measured using Western blot analysis. The hydro-ethanol extract of the aerial part possessed the highest phenolic (56.81 mg GAE/g) and flavonoid (63.92 mg RE/g) contents. In-depth profiling of the specialized metabolites by ultra-high-performance liquid chromatography—high-resolution mass spectrometry (UHPLC-HRMS) allowed for the identification and annotation of 65 compounds, including phenolic acids and glycosides, flavones, flavonols, chalcones, dihydrochalcones, and saponins. The hydro-ethanol extract of the aerial parts (132.65, 180.87, 172.46, and 108.37 mg TE/g, for the DPPH, ABTS, CUPRAC, and FRAP assays, respectively) and the ethanol extract of the rhizomes (415.06, 638.30, 477.77, and 301.02 mg TE/g, for the DPPH, ABTS, CUPRAC, and FRAP assays, respectively) exhibited the highest free radical scavenging and reducing activities. The ethanol and hydro-ethanol extracts of both the P. auriculata aerial part and rhizomes exhibited higher inhibitory activity against acetylcholinesterase, while the hydro-ethanol extracts (1.16 mmol ACAE/g, for both the aerial part and rhizomes extracts) were more active in the inhibition of α-glucosidase. After the treatment of an HT-29 colorectal cancer cell line with the extracts, the apoptosis mechanism was initiated, the integrity of the ECM was remodeled, and cell proliferation was also taken under control. In this way, Primula extracts were shown to be potential drug sources in the treatment of colorectal cancer. They were also detected as natural MMP inhibitors. The findings presented in the present study appraise the bioactivity of P. auriculata, an understudied species. Additional assessment is required to evaluate the cytotoxicity of P. auriculata as well as its activity in ex vivo systems.

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Pharmacology and toxicology of tannins

et al. 2022

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Influence of In Vitro Human Digestion Simulation on the Phenolics Contents and Biological Activities of the Aqueous Extracts from Turkish Cistus Species

Cited by 8 publications

References 53 publications

Polyphenol Fingerprint, Biological Activities, and In Silico Studies of the Medicinal Plant Cistus parviflorus L. Extract

Polyphenol Fingerprint, Biological Activities, and In Silico Studies of the Medicinal Plant Cistus parviflorus L. Extract

An In-Depth Study on the Metabolite Profile and Biological Properties of Primula auriculata Extracts: A Fascinating Sparkle on the Way from Nature to Functional Applications

Pharmacology and toxicology of tannins

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