Influence of Hydroxyapatite Nanoparticles and Surface Plasma Treatment on Bioactivity of Polycaprolactone Nanofibers

Šťastná, Eva; Částková, Klára; Ráheľ, Jozef

doi:10.3390/polym12091877

Cited by 21 publications

(14 citation statements)

References 41 publications

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“…Aragon et al used CH 2 Cl 2 /DMF as a solvent; HAp dispersion was stabilized by TWEEN ® 180, ES fibers contained 14% of HAp [ 31 ]. Stastna et al used a 3:1 mixture of acetic and formic acids as a solvent without addition of a surfactant [ 34 ]; the mechanical characteristics of ES mats were not studied.…”

Section: Resultsmentioning

confidence: 99%

“…A number of scientific articles reported notable results regrding the use of dense HAp/polyester composites impregnated with antibiotics [25][26][27][28][29], despite the fact that dense composites can hinder regeneration of the bone extracellular matrix. With that in mind, fibrous composite materials appear promising, however, the low compatibility of calcium phosphates with polyesters is especially visible in the case of attempts to make electrospun (ES) mats from the corresponding composite suspensions [30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Poly(ε-CL)/Hydroxyapatite Electrospun Fibers Reinforced by Poly(ε-CL)-b-poly(ethylene phosphoric acid)

Nifant’ev

Gavrilov

Tavtorkin

et al. 2021

IJMS

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In bone surgery and orthopedics, bioresorbable materials can be helpful in bone repair and countering post-op infections. Explicit antibacterial activity, osteoinductive and osteoconductive effects are essential to achieving this objective. Nonwoven electrospun (ES) fibers are receiving the close attention of physicians as promising materials for wound dressing and tissue engineering; potentially, in high contrast with dense materials, ES mats hamper regeneration of the bone extracellular matrix to a lesser extent. The use of the compositions of inherently biodegradable polyesters (poly(ε-caprolactone) PCL, poly(lactoglycolide), etc.), calcium phosphates and antibiotics is highly prospective, but the task of forming ES fibers from such compositions is complicated by the incompatibility of the main organic and inorganic ingredients, polyesters and calcium phosphates. In the present research we report the synthesis of hydroxyapatite (HAp) nanoparticles with uniform morphology, and demonstrate high efficiency of the block copolymer of PCL and poly(ethylene phosphoric acid) (PEPA) as an efficient compatibilizer for PCL/HAp mixtures that are able to form ES fibers with improved mechanical characteristics. The materials obtained in the presence of vancomycin exhibited incremental drug release against Staphylococcus aureus (St. aureus).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial Poly(ε-CL)/Hydroxyapatite Electrospun Fibers Reinforced by Poly(ε-CL)-b-poly(ethylene phosphoric acid)

Nifant’ev

Gavrilov

Tavtorkin

et al. 2021

IJMS

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“…According to the literature, PCL/elasin nanofibers (produced by the similar electrospinning parameters and electrospinning solution) possess smaller diameter compared with PCL/nHA fibers [ 30 , 31 ]. Therefor, the majority of the nanofibers with average diameter about 100 nm presented in the SEM images would have PCL/elastin composition.…”

Section: Discussionmentioning

confidence: 99%

Improved healing of critical-size femoral defect in osteoporosis rat models using 3D elastin/polycaprolactone/nHA scaffold in combination with mesenchymal stem cells

Hejazi

Ebrahimi

Asgary

et al. 2021

J Mater Sci: Mater Med

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Osteoporosis is a common bone disease that results in elevated risk of fracture, and delayed bone healing and impaired bone regeneration are implicated by this disease. In this study, Elastin/Polycaprolactone/nHA nanofibrous scaffold in combination with mesenchymal stem cells were used to regenerate bone defects. Cytotoxicity, cytocompatibility and cellular morphology were evaluated in vitro and observations revealed that an appropriate environment for cellular attachment, growth, migration, and proliferation is provided by this scaffold. At 3 months following ovariectomy (OVX), the rats were used as animal models with an induced critical size defect in the femur to evaluate the therapeutic potential of osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) seeded on 3 dimension (3D) scaffolds. In this experimental study, 24 female Wistar rats were equally divided into three groups: Control, scaffold (non-seeded BM-MSC), and scaffold + cell (seeded BM-MSC) groups. 30 days after surgery, the right femur was removed, and underwent a stereological analysis and RNA extraction in order to examine the expression of Bmp-2 and Vegf genes. The results showed a significant increase in stereological parameters and expression of Bmp-2 and Vegf in scaffold and scaffold + cell groups compared to the control rats. The present study suggests that the use of the 3D Elastin/Polycaprolactone (PCL)/Nano hydroxyapatite (nHA) scaffold in combination with MSCs may improve the fracture regeneration and accelerates bone healing at the osteotomy site in rats.

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“…On the contrary, a high load of HA could produce an aggregation of this component in the polymeric matrix, acting as weak points [43,44]. Both PEG and HA are well-known compounds that are frequently added to polymeric material formulations to produce hydrophilic and wettable materials [24,45]. In Figure 5 is shown the contact angle of a water drop on the surface of the PLA electrospun mats.…”

Section: Electrospinning Of Recovered Plamentioning

confidence: 99%

Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites

et al. 2021

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The design of scaffolds to reach similar three-dimensional structures mimicking the natural and fibrous environment of some cells is a challenge for tissue engineering, and 3D-printing and electrospinning highlights from other techniques in the production of scaffolds. The former is a well-known additive manufacturing technique devoted to the production of custom-made structures with mechanical properties similar to tissues and bones found in the human body, but lacks the resolution to produce small and interconnected structures. The latter is a well-studied technique to produce materials possessing a fibrillar structure, having the advantage of producing materials with tuned composition compared with a 3D-print. Taking the advantage that commercial 3D-printers work with polylactide (PLA) based filaments, a biocompatible and biodegradable polymer, in this work we produce PLA-based composites by blending materials obtained by 3D-printing and electrospinning. Porous PLA fibers have been obtained by the electrospinning of recovered PLA from 3D-printer filaments, tuning the mechanical properties by blending PLA with small amounts of polyethylene glycol and hydroxyapatite. A composite has been obtained by blending two layers of 3D-printed pieces with a central mat of PLA fibers. The composite presented a reduced storage modulus as compared with a single 3D-print piece and possessing similar mechanical properties to bone tissues. Furthermore, the biocompatibility of the composites is assessed by a simulated body fluid assay and by culturing composites with 3T3 fibroblasts. We observed that all these composites induce the growing and attaching of fibroblast over the surface of a 3D-printed layer and in the fibrous layer, showing the potential of commercial 3D-printers and filaments to produce scaffolds to be used in bone tissue engineering.

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Influence of Hydroxyapatite Nanoparticles and Surface Plasma Treatment on Bioactivity of Polycaprolactone Nanofibers

Cited by 21 publications

References 41 publications

Antibacterial Poly(ε-CL)/Hydroxyapatite Electrospun Fibers Reinforced by Poly(ε-CL)-b-poly(ethylene phosphoric acid)

Antibacterial Poly(ε-CL)/Hydroxyapatite Electrospun Fibers Reinforced by Poly(ε-CL)-b-poly(ethylene phosphoric acid)

Improved healing of critical-size femoral defect in osteoporosis rat models using 3D elastin/polycaprolactone/nHA scaffold in combination with mesenchymal stem cells

Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites

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