Influence of hydrodynamics on the growth kinetics of glass-adhering <i>Pseudomonas putida</i> cells through a parallel plate flow chamber

Mbaye, S.; Séchet, Philippe; Pignon, Frédéric; Martins, Jean M.F.

doi:10.1063/1.4821244

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…vasculature of living hosts, quiet river flows, medical devices[ 17 – 19 ]—the spectrum of flow effects on biofilm development is also broad. It includes initial attachment control[ 20 – 23 ], quorum sensing regulation[ 24 , 25 ], morphological changes[ 20 ], growth rate modification[ 26 – 29 ], metabolic switching[ 28 ], alteration of visco-elasticity[ 30 , 31 ], variation of extracellular matrix production, and possibly gene expression modification[ 11 , 32 ]. Some of these effects have clearly been shown to proceed by advection-diffusion mechanisms, e.g.…”

Section: Introductionmentioning

confidence: 99%

Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing

et al. 2017

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Bacterial communities attached to surfaces under fluid flow represent a widespread lifestyle of the microbial world. Through shear stress generation and molecular transport regulation, hydrodynamics conveys effects that are very different by nature but strongly coupled. To decipher the influence of these levers on bacterial biofilms immersed in moving fluids, we quantitatively and simultaneously investigated physicochemical and biological properties of the biofilm. We designed a millifluidic setup allowing to control hydrodynamic conditions and to monitor biofilm development in real time using microscope imaging. We also conducted a transcriptomic analysis to detect a potential physiological response to hydrodynamics. We discovered that a threshold value of shear stress determined biofilm settlement, with sub-piconewton forces sufficient to prevent biofilm initiation. As a consequence, distinct hydrodynamic conditions, which set spatial distribution of shear stress, promoted distinct colonization patterns with consequences on the growth mode. However, no direct impact of mechanical forces on biofilm growth rate was observed. Consistently, no mechanosensing gene emerged from our differential transcriptomic analysis comparing distinct hydrodynamic conditions. Instead, we found that hydrodynamic molecular transport crucially impacts biofilm growth by controlling oxygen availability. Our results shed light on biofilm response to hydrodynamics and open new avenues to achieve informed design of fluidic setups for investigating, engineering or fighting adherent communities.

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Section: Introductionmentioning

confidence: 99%

Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing

et al. 2017

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“…Tangential (shear) liquid flow can aid the bacteria by enhancing mass transfer of essential molecular species into the biofilm and preventing buildup of molecular byproducts. This has a pronounced impact on biofilm mechanical properties 1 and physical properties, [2][3][4][5] but also on the metabolism, 6 growth kinetics, 7,8 and biochemistry 9,10 of the resident microorganisms. The impact of time-changing flow on biofilm properties is less understood.…”

Section: Introductionmentioning

confidence: 99%

“…Tangential (shearing) liquid flow can aid the bacteria by enhancing mass transfer of essential molecular species into the biofilm and preventing buildup of molecular byproducts. This has a pronounced impact on biofilm mechanical properties and physical properties, − metabolism, growth kinetics, , and biochemistry. , The impact of time-changing flow on biofilm properties is less understood. , The effect of fluidic conditions on the biofilm is complicated by the fact that flow against it is expected to affect outer layers disproportionately, whereas portions at the attachment surface (the biointerface) remain relatively unperturbed. , For example, much of the biofilm resistance to elimination is linked to the surface attached portions, where highly attenuated molecular mass transfer of nutrients and oxygen may give rise to so-called “persister” cells. , Many biofilm species are also known to have localized anchoring points, resulting in mushroom-like structures . These are critical features that allow limited convection through the interior of the biofilm. , Biofilm structure and the influence of shear stress on it can be studied by optical microscopy, but the details at the attachment surface are hard to resolve due to interference from other strata.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic Effects on Biofilms at the Biointerface Using a Microfluidic Electrochemical Cell: Case Study of Pseudomonas sp.

Zarabadi¹,

Paquet-Mercier²,

Charette

et al. 2017

Langmuir

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The anchoring biofilm layer is expected to exhibit a different response to environmental stresses than for portions in the bulk, due to the protection from other strata and the proximity to the attachment surface. The effect of hydrodynamic stress on surface-adhered biofilm layers was tested using a specially designed microfluidic bio flow cell with an embedded three-electrode detection system. In situ electrochemical impedance spectroscopy (EIS) measurements of biocapacitance and bioresistance of Pseudomonas sp. biofilms were conducted during the growth phase and under different shear flow conditions with verification by other surface sensitive techniques. Distinct, but reversible changes to the amount of biofilm and its structure at the attachment surface were observed during the application of elevated shear stress. In contrast, regular microscopy revealed permanent distortion to the biofilm bulk, in the form of streamers and ripples. Following the application of extreme shear stresses, complete removal of significant portions of biofilm outer layers occurred, but this did not change the measured quantity of biofilm at the electrode attachment surface. The structure of the remaining biofilm, however, appeared to be modified and susceptible to further changes following application of shear stress directly to the unprotected biofilm layers at the attachment surface.

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“…ERDC/CERL TR-17-42 12 3 Results/Discussion 3.1 Selection of model microorganisms, P. putida F1 and S. oneidensis MR-1 P. putida F1 and S. oneidensis MR-1 were chosen as model organisms for the described methods development due to their ability to rapidly colonize and produce robust biofilms. Each genera consist of species that are known to form biofilms on smooth surfaces such as glass, which make them excellent candidates for attachment and growth on ITO (Waters et al 2008;Wang et al 2011;Mbaye et al 2013). Both organisms are commercially available, lack pathogenicity, and molecular probes that target unique regions of the 16S rRNA are well established (Greuter et al 2016).…”

Section: Epifluorescence Microscopy Of Hybridized Biofilms On Ito-coamentioning

confidence: 99%

Method for localizing and differentiating bacteria within biofilms grown on indium tin oxide : spatial distribution of exoelectrogenic bacteria within intact ITO biofilms via FISH

Arnett¹,

Lange²

2017

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With a limited supply of fossil fuel, there has been great interest in the development of new technologies that can take advantage of renewable fuel sources or convert energy stored in waste to usable energy. One such class of technologies are microbial fuel cells (MFCs), which can convert various carbohydrate rich sources as well as wastewater into electricity via biological catalysts. However, electrical current generation in these microbial driven systems is typically low making these technologies unsuitable for widespread use. In order for MFCs to become a viable alternative energy source, methods are needed to better understand the relationship between microbes and electron transfer. This work outlines a method for spatially differentiating exoelectrogenic bacteria within intact biofilms grown on a conductive surface. The technique involves the rapid generation of biofilms by using a drip flow bioreactor (DFR) on indium tin oxide (ITO)coated slides, in situ fixation of bacteria within the biofilms on the ITO surface, and determining species differentiation and location by probing with fluorescence in situ hybridization (FISH). This method was shown to effectively distinguish two exoelectrogens within biofilms on a conductive surface, which could serve as a novel means to study MFCs in greater detail. DISCLAIMER: The contents of this report are not to be used for advertising, publication, or promotional purposes. Citation of trade names does not constitute an official endorsement or approval of the use of such commercial products. All product names and trademarks cited are the property of their respective owners. The findings of this report are not to be construed as an official Department of the Army position unless so designated by other authorized documents.

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Influence of hydrodynamics on the growth kinetics of glass-adhering Pseudomonas putida cells through a parallel plate flow chamber

Cited by 8 publications

References 29 publications

Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing

Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing

Hydrodynamic Effects on Biofilms at the Biointerface Using a Microfluidic Electrochemical Cell: Case Study of Pseudomonas sp.

Method for localizing and differentiating bacteria within biofilms grown on indium tin oxide : spatial distribution of exoelectrogenic bacteria within intact ITO biofilms via FISH

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