Influence of host genotypes on growth, symbiotic performance and nitrogen assimilation in faba bean (Vicia faba L.) under salt stress

Abstract

A b s t r a c tFifteen genotypes of faba bean (Viciafaba L.) were inoculated with salt-tolerant Rhizobium leguminosarum biovar. viciae strain GRA19 in solution culture with 0 (control) and 75 mM NaC1 added immediately after transplanting. Genotypes varied in their tolerance of high levels of NaCI. Physiological parameters (dry weight of shoot and root, number and dry weight of nodules) were not affected by salinity in lines VF46, VF64 and VF112. Faba bean line VF60 was sensitive to salt stress. Host tolerance appeared to be a major requisite for nodulation and N2 fixation under salt stress. Tolerant line VF112 sustained nitrogen fixation under saline conditions. Activity of the ammonium assimilation enzymes glutamine synthetase and glutamate synthase, and soluble protein content, were reduced by salinity in all genotypes tested. Evidence presented here suggests a need to select faba bean genotypes that are tolerant to salt stress.Abbreviations: ARA -acetylene reduction activity, NADH-GOGAT -NADH-dependent glutamate synthase, GS -glutamine synthetase.

Beneficial Effects of Biochar-Based Organic Fertilizer on Nitrogen Assimilation, Antioxidant Capacities, and Photosynthesis of Sugar Beet (Beta vulgaris L.) under Saline-Alkaline Stress

“…These enzymes are inducible enzymes whose activity in roots can be increased by adequate inorganic nitrogen [46]. In this study, the activities of these enzymes in sugar beet roots were significantly reduced by saline-alkaline stress throughout the growth period (Figure 1), indicating that nitrogen assimilation of sugar beet roots was inhibited by saline-alkaline stress, as previously reported by Cordovilla et al [47]. Under saline-alkaline conditions, the application of biochar-based organic fertilizer improved the activities of NR and NiR (Figure 1A,B), especially during the late growth stages (110-130 DAS).…”

Faba Bean Cultivation – Revealing Novel Managing Practices for More Sustainable and Competitive European Cropping Systems

“…Measures undertaken to ameliorate the negative effects of salinity on faba bean plants include foliar application of silicon or inoculation with Pseudomonas fluorescens ( Hellal et al, 2012 ; Metwali et al, 2015 ). Salinity-tolerant faba bean genotypes are also available; one example is the line “VF112,” which has been reported as salt-tolerant because salt stress had no effect on its growth or nitrogen fixation ( del Pilar Cordovilla et al, 1995 ). Examples of genotypes with enhanced tolerance to abiotic stresses are presented in Table 2 .…”

SNP marker discovery, linkage map construction and identification of QTLs for enhanced salinity tolerance in field pea (Pisum sativumL.)

“…In terms of salinity tolerance, preliminary studies based on biomass reduction indicated that field pea is significantly more sensitive than other commonly cultivated Australian broad-acre crops such as barley [33,34], wheat [35] and canola [36], due to a low salinity threshold level [37] in pea. In comparison to other legumes, in contrast, pea [38-41], as well as faba bean [42], appear more tolerant than chickpea [43] and lentil [44]. …”